Detailed information NTG1148534

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PL963_RS04225
• Genome accession: NZ_LT962480
• Coordinates: 905297..906169 (-)
• Length: 290 a.a.
• NCBI ID: WP_103714216.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. syringae isolate CFBP4215
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 900297..911169

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PL963_RS04190 (CFBP4215_00792)	-	901082..902380 (-)	1299	WP_003430739.1	NAD(P)/FAD-dependent oxidoreductase	-
PL963_RS04200 (CFBP4215_00793)	-	902597..903130 (-)	534	WP_003317783.1	MOSC domain-containing protein	-
PL963_RS04205 (CFBP4215_00794)	-	903127..903756 (-)	630	WP_060400845.1	DUF1780 domain-containing protein	-
PL963_RS04210 (CFBP4215_00795)	-	903883..904344 (+)	462	WP_024643094.1	hypothetical protein	-
PL963_RS04215 (CFBP4215_00796)	yacG	904471..904680 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
PL963_RS04220 (CFBP4215_00797)	coaE	904677..905300 (-)	624	WP_004415092.1	dephospho-CoA kinase	-
PL963_RS04225 (CFBP4215_00798)	pilD	905297..906169 (-)	873	WP_103714216.1	prepilin peptidase	Machinery gene
PL963_RS04230 (CFBP4215_00799)	pilC	906171..907388 (-)	1218	WP_096104049.1	type II secretion system F family protein	Machinery gene
PL963_RS04235 (CFBP4215_00800)	pilB	907391..909085 (-)	1695	WP_104683674.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PL963_RS04240	-	909313..909747 (+)	435	WP_096104047.1	pilin	-
PL963_RS04245 (CFBP4215_00801)	-	909971..910516 (-)	546	WP_010415546.1	DUF1523 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31946.09 Da
• Isoelectric Point: 8.1558

>NTDB_id=1148534 PL963_RS04225 WP_103714216.1 905297..906169(-) (pilD) [Pseudomonas syringae pv. syringae isolate CFBP4215]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPIMMEHDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLGWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=1148534 PL963_RS04225 WP_103714216.1 905297..906169(-) (pilD) [Pseudomonas syringae pv. syringae isolate CFBP4215]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCCCCCCTGGCCTTCGTCACCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTACCGCCTGCCAATAATGATGGAGCACGACTGGAAAGCCCAATCCCGTGAACTGCTCGGCC
TGCCCGCCGAACCCGATCAGCCCGTCTTCAACCTGAACCGCCCCCGCTCCAGCTGCCCGCATTGCGCACACAAAATCCGC
CCTTGGGAGAACCTGCCGGTGATCAGCTATCTGCTGCTGCGTGGTAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCAGTGTTGTCGGCCTATGTGGCTTGGCATTTCGGTTTTGGCTGGCAGGCGGCGG
CGATGCTGGTATTGGGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGCTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCCTTCGGGCTGTTTACCTCGCTGAACGATGCGCTGTGGGGAGCGGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGGTTTTGCCGTTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTCGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.65	97.586	0.514
pilD	Vibrio campbellii strain DS40M4	52.297	97.586	0.51
pilD	Acinetobacter baumannii D1279779	52.555	94.483	0.497
pilD	Acinetobacter nosocomialis M2	51.825	94.483	0.49
pilD	Neisseria gonorrhoeae MS11	51.119	92.414	0.472