Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PL963_RS04235 Genome accession   NZ_LT962480
Coordinates   907391..909085 (-) Length   564 a.a.
NCBI ID   WP_104683674.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae isolate CFBP4215     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 902391..914085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL963_RS04200 (CFBP4215_00793) - 902597..903130 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  PL963_RS04205 (CFBP4215_00794) - 903127..903756 (-) 630 WP_060400845.1 DUF1780 domain-containing protein -
  PL963_RS04210 (CFBP4215_00795) - 903883..904344 (+) 462 WP_024643094.1 hypothetical protein -
  PL963_RS04215 (CFBP4215_00796) yacG 904471..904680 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PL963_RS04220 (CFBP4215_00797) coaE 904677..905300 (-) 624 WP_004415092.1 dephospho-CoA kinase -
  PL963_RS04225 (CFBP4215_00798) pilD 905297..906169 (-) 873 WP_103714216.1 prepilin peptidase Machinery gene
  PL963_RS04230 (CFBP4215_00799) pilC 906171..907388 (-) 1218 WP_096104049.1 type II secretion system F family protein Machinery gene
  PL963_RS04235 (CFBP4215_00800) pilB 907391..909085 (-) 1695 WP_104683674.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PL963_RS04240 - 909313..909747 (+) 435 WP_096104047.1 pilin -
  PL963_RS04245 (CFBP4215_00801) - 909971..910516 (-) 546 WP_010415546.1 DUF1523 family protein -
  PL963_RS04250 (CFBP4215_00802) - 910636..911634 (+) 999 WP_104683676.1 ATP-grasp domain-containing protein -
  PL963_RS04255 (CFBP4215_00803) - 911689..912828 (+) 1140 WP_104683677.1 trypsin-like peptidase domain-containing protein -
  PL963_RS04260 (CFBP4215_00804) - 912895..914028 (+) 1134 WP_024652521.1 phosphoribosyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62541.69 Da        Isoelectric Point: 5.9832

>NTDB_id=1148536 PL963_RS04235 WP_104683674.1 907391..909085(-) (pilB) [Pseudomonas syringae pv. syringae isolate CFBP4215]
MTDAVLSGLAKQLVLAELLTEPVAQQAYQQARREKISLVSYLVQNKLVKSLTLAEMASDQFGIPFMDLANLEKESQPKGL
VSEKLVRQHHALPLWRRGNRLFIGVSDPTNHQAVTDIQFSTGLNTEAILVEDDKLSNAIEKFFETGNGLGEMEDVDLGLE
VEQSDGKETSLADQNDADDAPVVRFVNKMLTDAIRLGSSDLHFEPYEKTFRVRLRTDGILHEVAKPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIKEGFPEAK
IDTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISSQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1148536 PL963_RS04235 WP_104683674.1 907391..909085(-) (pilB) [Pseudomonas syringae pv. syringae isolate CFBP4215]
ATGACTGACGCTGTGCTTTCTGGTTTGGCCAAACAACTGGTGCTCGCCGAGCTACTCACTGAACCTGTTGCTCAGCAAGC
GTACCAACAGGCGCGACGCGAAAAGATTTCGCTGGTCAGCTATCTGGTTCAGAACAAGTTAGTCAAAAGCCTGACGCTTG
CAGAGATGGCTTCTGACCAATTTGGCATTCCGTTCATGGACCTAGCGAACCTTGAGAAGGAAAGCCAGCCAAAAGGGCTG
GTAAGCGAAAAGCTGGTCCGACAACACCATGCACTCCCCCTGTGGAGACGCGGAAACAGGCTTTTTATAGGCGTATCCGA
CCCAACGAACCATCAGGCAGTGACTGATATCCAGTTCAGCACCGGTCTAAATACCGAGGCGATACTTGTAGAGGACGACA
AACTTAGCAATGCCATCGAAAAGTTCTTTGAGACAGGCAATGGCTTGGGAGAGATGGAAGACGTTGATCTCGGCCTGGAA
GTTGAACAATCCGATGGCAAAGAGACCTCGCTTGCTGATCAGAATGATGCTGACGACGCTCCTGTAGTGCGCTTCGTGAA
CAAAATGTTGACGGACGCTATCCGCCTCGGCTCTTCGGACCTGCATTTTGAACCTTACGAAAAGACCTTTCGTGTGCGCC
TGCGTACCGACGGCATCCTGCATGAAGTCGCCAAGCCGCCGATCCATTTGGCGAACCGTATTGCCGCGCGTTTAAAAGTT
ATGGCCAGCCTCGATATCTCGGAACGACGCAAACCGCAGGACGGCAGGGTAAAGCTGCGAGTGTCCAAAACCAAGGCTAT
CGACTTTCGTATGAACACCTTGCCTACGCTGTGGGGCGAGAAAATCGTGATGCGGATTCTCGATCCAACCAGCGCGCAGA
TGGGTATCGATGCACTCGGTTACGAGCCCGAGCAAAAGGCGCTATATCTCGAAGCGTTGAAACAACCGCAAGGCATGATT
CTGGTCACAGGACCTACAGGGTCGGGTAAAACTGTTTCGCTTTATACCGGCCTGAACATTCTCAACACAGTGGATATCAA
TATTTCCACCGCCGAGGATCCAGTAGAAATCAATCTGGAAGGCATCAATCAGGTCAACGTCAATCCGCGCCAAGGTCTGG
ATTTTTCACAAGCATTGCGGGCTTTTCTGCGTCAGGATCCTGACGTAATTATGGTCGGCGAGATTCGAGACCTGGAAACT
GCCGAGATCGCCATCAAGGCATCACAGACCGGCCATATGGTGTTATCGACCCTCCACACCAACAGCGCAGCAGAAACGCT
GACCCGCCTGCATCACATGGGCGTCGCAGCGTTCAACATTGCCACTGCGATCAATTTGATTATCGCGCAACGACTCGCTC
GCAAATTATGTAGCCATTGCAAGAAAGAACTCGATATTCCTCGCGAAACGCTGATTAAAGAAGGGTTTCCAGAAGCGAAA
ATTGACACTTTCAAAATCTACGGCCCGGTGGGCTGTGAGCATTGCAATGGAGGCTATAGAGGAAGGGTGGGCATTTACGA
AGTGGTCAAGAAGACGCCAGAGCTGGAACGGATCATCATGGAGGAAGGTAACTCGTTGGAGATATCCAGTCAGATGCGCA
AGGACGGCTTTAATGATCTGCGTACCTCAGGCCTTTCAAAGGCCATGCAGGGCATCACAAGCCTAGAAGAAGTCAACCGT
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.774

100

0.58
  pilB Acinetobacter baumannii D1279779

57.394

100

0.578
  pilB Legionella pneumophila strain ERS1305867

55.752

100

0.559
  pilB Vibrio cholerae strain A1552

50.534

99.645

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.727

97.518

0.495
  pilB Vibrio campbellii strain DS40M4

49.113

100

0.491
  pilF Neisseria gonorrhoeae MS11

48.485

99.468

0.482
  pilF Thermus thermophilus HB27

39.046

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.931

92.908

0.362

Multiple sequence alignment