Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MFPB19_RS06905 Genome accession   NZ_LT960784
Coordinates   1364913..1365803 (-) Length   296 a.a.
NCBI ID   WP_099713231.1    Uniprot ID   -
Organism   Latilactobacillus sakei isolate MFPB19     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1359913..1370803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFPB19_RS06870 (MFPB19_1434) - 1360979..1361989 (-) 1011 WP_025016388.1 class I SAM-dependent methyltransferase -
  MFPB19_RS06875 (MFPB19_1435) - 1362113..1362451 (-) 339 WP_056947123.1 hypothetical protein -
  MFPB19_RS06880 (MFPB19_1436) comGF 1362414..1362926 (-) 513 WP_025016386.1 competence type IV pilus minor pilin ComGF Machinery gene
  MFPB19_RS06885 (MFPB19_1437) comGE 1362901..1363206 (-) 306 WP_025016385.1 hypothetical protein Machinery gene
  MFPB19_RS06890 (MFPB19_1438) comGD 1363193..1363645 (-) 453 WP_056947126.1 competence type IV pilus minor pilin ComGD Machinery gene
  MFPB19_RS06895 (MFPB19_1439) comGC 1363617..1363916 (-) 300 WP_056947129.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MFPB19_RS06900 (MFPB19_1440) comGB 1363913..1364920 (-) 1008 WP_099713232.1 type II secretion system F family protein Machinery gene
  MFPB19_RS06905 (MFPB19_1441) comGA 1364913..1365803 (-) 891 WP_099713231.1 competence type IV pilus ATPase ComGA Machinery gene
  MFPB19_RS06910 (MFPB19_1442) - 1365918..1366649 (-) 732 WP_076632059.1 YebC/PmpR family DNA-binding transcriptional regulator -
  MFPB19_RS06915 (MFPB19_1443) - 1366740..1367240 (-) 501 WP_076632058.1 VanZ family protein -
  MFPB19_RS06920 (MFPB19_1444) - 1367337..1367711 (+) 375 WP_011375004.1 hypothetical protein -
  MFPB19_RS06930 (MFPB19_1445) - 1367898..1369298 (-) 1401 WP_076632055.1 hypothetical protein -
  MFPB19_RS06935 (MFPB19_1446) - 1369298..1370368 (-) 1071 WP_142501005.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32921.78 Da        Isoelectric Point: 6.7477

>NTDB_id=1148442 MFPB19_RS06905 WP_099713231.1 1364913..1365803(-) (comGA) [Latilactobacillus sakei isolate MFPB19]
MSTEEKLTTLLKHCAKQRVQDLYFTPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRQAGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPNTLIIGEIRDLETAQYAVSAALSGHLVLSTIHARSTQG
VVARLLDLGISELQLRACLTGIAYQSLVTKDDVVQAHYELLTGPDYFSKKEGGCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=1148442 MFPB19_RS06905 WP_099713231.1 1364913..1365803(-) (comGA) [Latilactobacillus sakei isolate MFPB19]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAACAACGTGTTCAAGATCTTTATTTTACACCGAC
AATAGGTGGCTGGCGCTTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCGCAAACCGGCCGCTCGGAATTA
CAGCTAGCCGATCAAACAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAACCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATCTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGACAGGCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGTAAAACGACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTTAATGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGAATACGCTGATTATCGGTGAAATTCGCGATTTAG
AGACAGCTCAATATGCAGTTTCTGCGGCCTTAAGTGGCCACCTGGTATTGAGTACTATTCACGCTCGGAGTACACAGGGC
GTGGTCGCGCGCTTATTAGATCTTGGTATTTCAGAGCTGCAATTGCGGGCCTGTCTAACGGGAATTGCCTACCAATCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTCCAGATTATTTCTCAAAAAAAGAAGGTGGTT
GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

98.649

100

0.986
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.887

89.527

0.375
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.887

89.527

0.375
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.887

89.527

0.375
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.887

89.527

0.375
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

40.511

92.568

0.375
  comGA Lactococcus lactis subsp. cremoris KW2

37.248

100

0.375
  comYA Streptococcus gordonii str. Challis substr. CH1

40.221

91.554

0.368

Multiple sequence alignment