Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   MFPB19_RS06880 Genome accession   NZ_LT960784
Coordinates   1362414..1362926 (-) Length   170 a.a.
NCBI ID   WP_025016386.1    Uniprot ID   -
Organism   Latilactobacillus sakei isolate MFPB19     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1357414..1367926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFPB19_RS06850 (MFPB19_1430) - 1358340..1358705 (+) 366 WP_011374991.1 DUF805 domain-containing protein -
  MFPB19_RS06860 (MFPB19_1432) - 1359248..1359706 (-) 459 WP_056947121.1 hypothetical protein -
  MFPB19_RS06865 (MFPB19_1433) - 1359761..1360957 (-) 1197 WP_011374993.1 acetate/propionate family kinase -
  MFPB19_RS06870 (MFPB19_1434) - 1360979..1361989 (-) 1011 WP_025016388.1 class I SAM-dependent methyltransferase -
  MFPB19_RS06875 (MFPB19_1435) - 1362113..1362451 (-) 339 WP_056947123.1 hypothetical protein -
  MFPB19_RS06880 (MFPB19_1436) comGF 1362414..1362926 (-) 513 WP_025016386.1 competence type IV pilus minor pilin ComGF Machinery gene
  MFPB19_RS06885 (MFPB19_1437) comGE 1362901..1363206 (-) 306 WP_025016385.1 hypothetical protein Machinery gene
  MFPB19_RS06890 (MFPB19_1438) comGD 1363193..1363645 (-) 453 WP_056947126.1 competence type IV pilus minor pilin ComGD Machinery gene
  MFPB19_RS06895 (MFPB19_1439) comGC 1363617..1363916 (-) 300 WP_056947129.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MFPB19_RS06900 (MFPB19_1440) comGB 1363913..1364920 (-) 1008 WP_099713232.1 type II secretion system F family protein Machinery gene
  MFPB19_RS06905 (MFPB19_1441) comGA 1364913..1365803 (-) 891 WP_099713231.1 competence type IV pilus ATPase ComGA Machinery gene
  MFPB19_RS06910 (MFPB19_1442) - 1365918..1366649 (-) 732 WP_076632059.1 YebC/PmpR family DNA-binding transcriptional regulator -
  MFPB19_RS06915 (MFPB19_1443) - 1366740..1367240 (-) 501 WP_076632058.1 VanZ family protein -
  MFPB19_RS06920 (MFPB19_1444) - 1367337..1367711 (+) 375 WP_011375004.1 hypothetical protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19690.57 Da        Isoelectric Point: 6.6472

>NTDB_id=1148437 MFPB19_RS06880 WP_025016386.1 1362414..1362926(-) (comGF) [Latilactobacillus sakei isolate MFPB19]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLDGYMPLLGNLKQLQLDYQRPFVQIDATFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=1148437 MFPB19_RS06880 WP_025016386.1 1362414..1362926(-) (comGF) [Latilactobacillus sakei isolate MFPB19]
TTGCGATTAATTGGACGACCGATTAAACACTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTGACACAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCAGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTCGTTGTCAGCCAATTAGATGGGTATATGCCATTATTAGGTAACCTCAAGCAACTTCAACTTGACTACCAGC
GACCGTTTGTTCAAATCGATGCGACTTTTACGGATCAGAGTCGCTACCAACACGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATTAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

98.824

100

0.988

Multiple sequence alignment