Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LSAJ64_RS07450 Genome accession   NZ_LT960781
Coordinates   1443517..1444407 (-) Length   296 a.a.
NCBI ID   WP_016265378.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain J64     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1438517..1449407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSAJ64_RS07415 (LSAJ64_1498) - 1439583..1440593 (-) 1011 WP_099947695.1 class I SAM-dependent methyltransferase -
  LSAJ64_RS07420 (LSAJ64_1499) - 1440717..1441055 (-) 339 WP_035145011.1 hypothetical protein -
  LSAJ64_RS07425 (LSAJ64_1500) comGF 1441018..1441530 (-) 513 WP_101377989.1 competence type IV pilus minor pilin ComGF Machinery gene
  LSAJ64_RS07430 (LSAJ64_1501) comGE 1441505..1441810 (-) 306 WP_099960315.1 type II secretion system protein Machinery gene
  LSAJ64_RS07435 (LSAJ64_1502) comGD 1441797..1442249 (-) 453 WP_099960316.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LSAJ64_RS07440 (LSAJ64_1503) comGC 1442221..1442520 (-) 300 WP_099960317.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LSAJ64_RS07445 (LSAJ64_1504) comGB 1442517..1443524 (-) 1008 WP_101377990.1 type II secretion system F family protein Machinery gene
  LSAJ64_RS07450 (LSAJ64_1505) comGA 1443517..1444407 (-) 891 WP_016265378.1 competence type IV pilus ATPase ComGA Machinery gene
  LSAJ64_RS07455 (LSAJ64_1506) - 1444522..1445253 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  LSAJ64_RS07460 (LSAJ64_1507) - 1445344..1445844 (-) 501 WP_099947696.1 VanZ family protein -
  LSAJ64_RS07465 (LSAJ64_1508) - 1445941..1446315 (+) 375 WP_011375004.1 hypothetical protein -
  LSAJ64_RS07475 (LSAJ64_1509) - 1446502..1447902 (-) 1401 WP_099947697.1 hypothetical protein -
  LSAJ64_RS07480 (LSAJ64_1510) - 1447902..1448972 (-) 1071 WP_142501005.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32972.78 Da        Isoelectric Point: 6.3840

>NTDB_id=1148405 LSAJ64_RS07450 WP_016265378.1 1443517..1444407(-) (comGA) [Latilactobacillus sakei strain J64]
MSTEEKLTTLLKHCAKHYVQDLYFTPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLADQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRQSGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYVVSAALSGHLVLSTIHARSTQG
VVARLLDLGISELQLRACLTGIAYQSLVTKDDVVQAHYELLTGSDYFSKKEGGCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=1148405 LSAJ64_RS07450 WP_016265378.1 1443517..1444407(-) (comGA) [Latilactobacillus sakei strain J64]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAGCACTATGTGCAAGATCTTTATTTTACACCGAC
AATAGGTGGCTGGCGCTTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCGCAAACCGGCCGCTCGGAATTA
CAGCTAGCCGATCAAACAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAACCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATCTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGACAGTCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGTAAAACGACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTCAACGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGGATACGCTGATTATCGGTGAAATTCGCGATTTAG
AGACAGCTCAATACGTGGTTTCTGCGGCCTTAAGTGGCCACCTGGTATTGAGTACTATTCACGCTCGGAGTACACAGGGC
GTGGTCGCGCGCTTATTAGATCTTGGTATTTCTGAGCTGCAGTTGCGGGCCTGTCTAACGGGAATTGCCTACCAATCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTTCAGATTATTTCTCAAAAAAAGAAGGTGGTT
GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

99.324

100

0.993
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

42.279

91.892

0.389
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.912

91.892

0.385
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.912

91.892

0.385
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.912

91.892

0.385
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.912

91.892

0.385
  comGA Lactococcus lactis subsp. cremoris KW2

37.793

100

0.382
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

41.241

92.568

0.382
  comYA Streptococcus gordonii str. Challis substr. CH1

40.809

91.892

0.375
  comYA Streptococcus mutans UA140

38.909

92.905

0.361
  comYA Streptococcus mutans UA159

38.909

92.905

0.361

Multiple sequence alignment