Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   VTAP4600_RS01260 Genome accession   NZ_LT960611
Coordinates   263856..265088 (-) Length   410 a.a.
NCBI ID   WP_102521138.1    Uniprot ID   A0A2N8Z8L8
Organism   Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 258856..270088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VTAP4600_RS01235 (VTAP4600_A0246) mutT 260676..261083 (+) 408 WP_102521133.1 8-oxo-dGTP diphosphatase MutT -
  VTAP4600_RS01240 (VTAP4600_A0247) yacG 261272..261481 (-) 210 WP_102521134.1 DNA gyrase inhibitor YacG -
  VTAP4600_RS01245 (VTAP4600_A0248) zapD 261506..262246 (-) 741 WP_102521135.1 cell division protein ZapD -
  VTAP4600_RS01250 (VTAP4600_A0249) coaE 262288..262887 (-) 600 WP_102521136.1 dephospho-CoA kinase -
  VTAP4600_RS01255 (VTAP4600_A0250) pilD 262899..263774 (-) 876 WP_102521137.1 prepilin peptidase Machinery gene
  VTAP4600_RS01260 (VTAP4600_A0251) pilC 263856..265088 (-) 1233 WP_102521138.1 type II secretion system F family protein Machinery gene
  VTAP4600_RS01265 (VTAP4600_A0252) pilB 265098..266783 (-) 1686 WP_102521139.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VTAP4600_RS01270 (VTAP4600_A0253) pilA 266783..267253 (-) 471 WP_102521140.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VTAP4600_RS01275 (VTAP4600_A0254) nadC 267487..268374 (-) 888 WP_102521141.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VTAP4600_RS01280 (VTAP4600_A0255) ampD 268543..269088 (+) 546 WP_102521142.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45698.98 Da        Isoelectric Point: 10.1427

>NTDB_id=1148300 VTAP4600_RS01260 WP_102521138.1 263856..265088(-) (pilC) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
MDSTSFYRLKLHNFRWRGHNSSGKKVSGQILAYTECEVRDRLSNQKIHIQKIKRRSVSFFERVSNKVSAKDILIFSRQMA
TMLDAGVPMIQALKMLMDNQNKAEMKSILRQLSSAVEAGTPLSRALATTSIHFDNFYVDLIATGEQTGHLSQIFDRLATY
QEKSALLKSKVKKAMIYPAMVSFTALLVSYLMLTFVIPEFETMFAGLGANLPWFTQQVLNLSHVVQAQGWKLFCLLIALT
LCLRAAKKRSYALRLIIARYVLVLPIIGAVLTKASLAKFSRTLAISFSSGIPILSGIETSSKTANNLYFQKVITKVLQDT
AAGMPIYLAMRNSNAFPEMMLQMVMIGEESGRLDNMLNKVADVYENEVDDTVDNLGKLLEPIIILVLGGLIGGLVIAMYL
PIFNLMSVIG

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=1148300 VTAP4600_RS01260 WP_102521138.1 263856..265088(-) (pilC) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
ATGGATTCCACTTCGTTTTATCGCTTAAAGCTGCATAACTTCCGATGGCGTGGGCACAATAGCTCAGGTAAAAAAGTCTC
AGGACAGATCCTGGCTTACACAGAGTGTGAAGTAAGGGATCGGCTTTCTAATCAAAAAATACACATACAAAAAATCAAAC
GCCGCAGTGTTTCTTTTTTTGAAAGAGTCTCCAACAAAGTCAGTGCGAAGGACATTCTCATTTTCAGTCGTCAGATGGCC
ACCATGCTTGATGCTGGTGTTCCCATGATACAAGCCCTAAAAATGCTGATGGATAACCAAAACAAAGCCGAGATGAAGTC
TATACTGCGCCAATTATCTAGCGCGGTTGAAGCGGGAACGCCACTTTCTCGAGCGCTTGCAACGACATCGATCCATTTTG
ACAACTTCTACGTCGATCTCATCGCAACGGGTGAACAAACCGGTCATCTTTCGCAGATATTCGACCGGCTGGCGACTTAT
CAAGAAAAAAGTGCATTGCTGAAATCAAAAGTAAAAAAGGCCATGATTTACCCCGCGATGGTGAGCTTTACGGCTCTACT
GGTATCCTACTTAATGCTGACGTTTGTGATCCCAGAATTTGAAACCATGTTCGCAGGATTAGGAGCCAATTTACCTTGGT
TTACTCAGCAAGTCTTAAATCTTTCTCATGTGGTTCAAGCGCAAGGCTGGAAGCTTTTTTGCCTATTGATTGCTCTCACC
CTTTGCTTACGCGCTGCAAAAAAGCGTTCCTATGCGCTTAGGCTAATCATCGCTCGCTACGTTTTGGTCTTGCCGATTAT
CGGAGCGGTACTCACTAAAGCTTCCCTTGCCAAGTTCAGTCGTACCTTAGCCATCAGTTTTAGCTCAGGAATTCCGATTC
TATCCGGCATAGAAACCAGTTCAAAAACCGCAAATAATCTCTATTTCCAAAAGGTCATCACCAAAGTTCTGCAAGATACT
GCCGCGGGAATGCCTATCTATTTGGCAATGAGAAACAGCAATGCCTTCCCTGAAATGATGCTGCAAATGGTCATGATCGG
AGAAGAGTCTGGCCGCTTAGACAACATGCTCAATAAAGTAGCAGACGTCTACGAAAATGAGGTCGACGATACTGTCGATA
ACTTAGGTAAACTGCTAGAACCCATCATAATTTTGGTTTTAGGAGGGCTAATTGGAGGATTGGTCATCGCAATGTACCTT
CCGATCTTTAACTTAATGAGCGTGATAGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8Z8L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

62.438

98.049

0.612
  pilC Vibrio campbellii strain DS40M4

61.097

97.805

0.598
  pilC Acinetobacter baylyi ADP1

41.457

97.073

0.402
  pilC Acinetobacter baumannii D1279779

40.75

97.561

0.398
  pilC Legionella pneumophila strain ERS1305867

38.847

97.317

0.378
  pilC Pseudomonas stutzeri DSM 10701

39.141

96.585

0.378
  pilG Neisseria meningitidis 44/76-A

37.408

99.756

0.373
  pilG Neisseria gonorrhoeae MS11

37.164

99.756

0.371

Multiple sequence alignment