Detailed information NTG1148299

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: VTAP4600_RS01255
• Genome accession: NZ_LT960611
• Coordinates: 262899..263774 (-)
• Length: 291 a.a.
• NCBI ID: WP_102521137.1
• Uniprot ID: A0A2N8Z8I3
• Organism: Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 257899..268774

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VTAP4600_RS01235 (VTAP4600_A0246)	mutT	260676..261083 (+)	408	WP_102521133.1	8-oxo-dGTP diphosphatase MutT	-
VTAP4600_RS01240 (VTAP4600_A0247)	yacG	261272..261481 (-)	210	WP_102521134.1	DNA gyrase inhibitor YacG	-
VTAP4600_RS01245 (VTAP4600_A0248)	zapD	261506..262246 (-)	741	WP_102521135.1	cell division protein ZapD	-
VTAP4600_RS01250 (VTAP4600_A0249)	coaE	262288..262887 (-)	600	WP_102521136.1	dephospho-CoA kinase	-
VTAP4600_RS01255 (VTAP4600_A0250)	pilD	262899..263774 (-)	876	WP_102521137.1	prepilin peptidase	Machinery gene
VTAP4600_RS01260 (VTAP4600_A0251)	pilC	263856..265088 (-)	1233	WP_102521138.1	type II secretion system F family protein	Machinery gene
VTAP4600_RS01265 (VTAP4600_A0252)	pilB	265098..266783 (-)	1686	WP_102521139.1	type IV-A pilus assembly ATPase PilB	Machinery gene
VTAP4600_RS01270 (VTAP4600_A0253)	pilA	266783..267253 (-)	471	WP_102521140.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
VTAP4600_RS01275 (VTAP4600_A0254)	nadC	267487..268374 (-)	888	WP_102521141.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32654.78 Da
• Isoelectric Point: 5.7785

>NTDB_id=1148299 VTAP4600_RS01255 WP_102521137.1 262899..263774(-) (pilD) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
MEAFYYYPWLYPLFATIFGLMIGSFLNVVIYRTPLMMEREWKQEFTESYPELCPDAKIDTTPLSLSTPRSTCPKCQHQLS
ISDNIPVISWLFLKGKCRYCETKISVRYPAVEILTATLSLVIALSFPLSLYSVALLFFTFTLIAATFIDLDTLLLPDQLT
LPLMWSGIALALVGESPLSLQDSVIGAMAGYLCLWSVYKVFKLLTGKDGMGYGDFKLLAALGAWLGWQSLPMIVLMSSLV
GLVFGLIQLRLQRKGIEHVFPFGPYLAIAGWLTLLFGSEIANWYFTNILGI

Nucleotide

• Length: 876 bp

>NTDB_id=1148299 VTAP4600_RS01255 WP_102521137.1 262899..263774(-) (pilD) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
ATGGAAGCGTTTTACTACTACCCTTGGCTATATCCTTTATTCGCCACTATATTTGGCCTAATGATAGGCAGTTTCCTTAA
TGTCGTGATCTATCGAACGCCTCTTATGATGGAGCGTGAATGGAAACAAGAGTTTACCGAATCCTATCCCGAACTTTGCC
CTGATGCAAAGATCGATACAACACCATTAAGCCTGAGCACTCCGAGATCGACCTGCCCTAAATGTCAGCATCAATTAAGT
ATCTCAGACAATATCCCGGTAATTAGCTGGCTATTTTTGAAAGGAAAATGCCGCTACTGCGAAACTAAAATCAGTGTTCG
CTACCCAGCGGTAGAAATACTGACAGCCACATTGTCGTTAGTTATCGCTCTGAGCTTCCCACTCAGCCTTTATAGTGTCG
CTCTATTATTTTTTACCTTTACGCTCATCGCTGCTACTTTCATCGATCTCGACACTCTACTGCTTCCAGACCAACTCACA
TTGCCATTAATGTGGTCAGGGATCGCACTGGCCCTGGTAGGCGAAAGCCCACTCAGCTTACAAGACTCGGTCATCGGTGC
CATGGCTGGGTATCTGTGTTTATGGTCAGTTTACAAAGTATTCAAGCTATTAACGGGCAAAGATGGCATGGGTTACGGGG
ACTTTAAACTGCTCGCCGCACTAGGAGCATGGCTTGGGTGGCAAAGTCTGCCAATGATTGTGCTTATGTCCTCTTTGGTC
GGATTAGTCTTTGGTCTCATTCAACTGCGCTTACAGCGTAAGGGCATTGAACACGTTTTTCCTTTTGGCCCATATCTGGC
CATCGCTGGCTGGTTAACATTGCTATTTGGTAGCGAAATCGCAAATTGGTACTTCACCAACATTCTAGGTATATAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-246)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-126)

3D structure

• AlphaFold DB: A0A2N8Z8I3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	69.759	100	0.698
pilD	Vibrio cholerae strain A1552	66.212	100	0.667
pilD	Acinetobacter baumannii D1279779	51.493	92.096	0.474
pilD	Acinetobacter nosocomialis M2	50.746	92.096	0.467
pilD	Neisseria gonorrhoeae MS11	45.23	97.251	0.44