Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VTAP4600_RS01270 Genome accession   NZ_LT960611
Coordinates   266783..267253 (-) Length   156 a.a.
NCBI ID   WP_102521140.1    Uniprot ID   -
Organism   Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 261783..272253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VTAP4600_RS01250 (VTAP4600_A0249) coaE 262288..262887 (-) 600 WP_102521136.1 dephospho-CoA kinase -
  VTAP4600_RS01255 (VTAP4600_A0250) pilD 262899..263774 (-) 876 WP_102521137.1 prepilin peptidase Machinery gene
  VTAP4600_RS01260 (VTAP4600_A0251) pilC 263856..265088 (-) 1233 WP_102521138.1 type II secretion system F family protein Machinery gene
  VTAP4600_RS01265 (VTAP4600_A0252) pilB 265098..266783 (-) 1686 WP_102521139.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VTAP4600_RS01270 (VTAP4600_A0253) pilA 266783..267253 (-) 471 WP_102521140.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VTAP4600_RS01275 (VTAP4600_A0254) nadC 267487..268374 (-) 888 WP_102521141.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VTAP4600_RS01280 (VTAP4600_A0255) ampD 268543..269088 (+) 546 WP_102521142.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VTAP4600_RS01285 (VTAP4600_A0256) pdhR 269381..270148 (+) 768 WP_102521143.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16693.09 Da        Isoelectric Point: 8.0993

>NTDB_id=1148302 VTAP4600_RS01270 WP_102521140.1 266783..267253(-) (pilA) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
MEKKMKNKQQGFTLIELMIVVAIVAILSSFAVPAYQNYTKKATLAEFPKAASAIKLAVEVCAHESSADSDSFKTNCISNN
NGVPAQFTLNKMQIVAAAGSSSGAVDIRVKATSDKGPIKTGETYVMTASYQAEGITWDSKCYKDAGMSQANTEYCP

Nucleotide


Download         Length: 471 bp        

>NTDB_id=1148302 VTAP4600_RS01270 WP_102521140.1 266783..267253(-) (pilA) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
ATGGAGAAGAAAATGAAAAATAAACAACAAGGCTTTACGCTCATTGAGTTGATGATTGTGGTGGCGATCGTAGCTATTCT
TTCGAGTTTTGCCGTTCCCGCTTACCAAAACTACACAAAAAAGGCGACACTTGCCGAATTCCCTAAAGCTGCTTCAGCGA
TAAAATTAGCGGTAGAAGTTTGTGCTCATGAGAGCTCCGCTGATAGCGATAGCTTCAAAACCAACTGTATTTCAAATAAT
AATGGGGTACCAGCTCAATTCACTCTAAATAAAATGCAGATTGTAGCTGCTGCAGGAAGTAGTTCAGGTGCTGTCGATAT
TCGTGTAAAAGCAACTTCAGATAAAGGACCAATAAAAACAGGCGAAACCTATGTTATGACAGCTAGTTACCAAGCCGAAG
GGATAACTTGGGACTCCAAATGTTATAAAGACGCAGGTATGAGTCAAGCTAACACTGAATATTGCCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

58.974

100

0.59

Multiple sequence alignment