Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VTAP4600_RS01265 Genome accession   NZ_LT960611
Coordinates   265098..266783 (-) Length   561 a.a.
NCBI ID   WP_102521139.1    Uniprot ID   A0A2N8Z8J5
Organism   Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 260098..271783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VTAP4600_RS01235 (VTAP4600_A0246) mutT 260676..261083 (+) 408 WP_102521133.1 8-oxo-dGTP diphosphatase MutT -
  VTAP4600_RS01240 (VTAP4600_A0247) yacG 261272..261481 (-) 210 WP_102521134.1 DNA gyrase inhibitor YacG -
  VTAP4600_RS01245 (VTAP4600_A0248) zapD 261506..262246 (-) 741 WP_102521135.1 cell division protein ZapD -
  VTAP4600_RS01250 (VTAP4600_A0249) coaE 262288..262887 (-) 600 WP_102521136.1 dephospho-CoA kinase -
  VTAP4600_RS01255 (VTAP4600_A0250) pilD 262899..263774 (-) 876 WP_102521137.1 prepilin peptidase Machinery gene
  VTAP4600_RS01260 (VTAP4600_A0251) pilC 263856..265088 (-) 1233 WP_102521138.1 type II secretion system F family protein Machinery gene
  VTAP4600_RS01265 (VTAP4600_A0252) pilB 265098..266783 (-) 1686 WP_102521139.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VTAP4600_RS01270 (VTAP4600_A0253) pilA 266783..267253 (-) 471 WP_102521140.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VTAP4600_RS01275 (VTAP4600_A0254) nadC 267487..268374 (-) 888 WP_102521141.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VTAP4600_RS01280 (VTAP4600_A0255) ampD 268543..269088 (+) 546 WP_102521142.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VTAP4600_RS01285 (VTAP4600_A0256) pdhR 269381..270148 (+) 768 WP_102521143.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62697.16 Da        Isoelectric Point: 6.1116

>NTDB_id=1148301 VTAP4600_RS01265 WP_102521139.1 265098..266783(-) (pilB) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
MYSLIPILAHAGLITLEHEIELRDHLSTSETSVCEALNELGFLSPALLLQKLSQLFGLRIENPLDYPYSALCKKLGVREL
ISRQQALPLYQNGQLLCVAISDPSNTEIEEEFGFATNLSIELVLCEEKQLRACIKRLYGNLIDSDDGAGRDINEQDLAEL
VTVSDNEHQQNEDLSKDTSPVSRFIHQVLLEAIRKNASDIHFEPYEESYRIRMRCDGMLLQTNSPPSQLNRRLAARLKIL
AKLDIAERRLPQDGRIKLHLNANTSIDLRVSTLPTLWGEKVVLRLLDGDNAKLDINALGYSEQQKQIYLNALSKPQGMIL
MTGPTGSGKTISLYTGLSKLNKIERNISTAEDPVEINFPGINQVQIQPKVGFTFAKALRAFLRQDPDVVMLGEIRDLETA
EIAIKAAQTGHLVLSTLHTNSAAETIIRLQNMGIEAYNLASSLSLVVAQRLARRLCPQCKTEHRMPFAMRQALDIGQDCR
IYQANKEGCNDCNAGYSGRIGIYEMLQINDQLIEAIAKRLSMPELEAIAVQHGMITLRQAGIDKLKLGITSHQELQRVLF
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1148301 VTAP4600_RS01265 WP_102521139.1 265098..266783(-) (pilB) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
ATGTACAGCCTAATTCCTATTCTTGCTCATGCAGGCCTCATTACACTTGAGCATGAAATTGAATTGAGAGATCACCTTAG
CACCTCAGAAACATCCGTTTGTGAGGCGCTAAATGAGCTAGGATTTCTGTCACCTGCATTATTACTTCAAAAGCTAAGCC
AACTGTTTGGCCTACGCATTGAAAATCCATTGGACTACCCCTACTCCGCTTTATGTAAGAAGCTGGGCGTCAGAGAATTG
ATCTCTCGCCAGCAGGCGCTGCCGCTCTATCAAAACGGTCAACTGCTTTGTGTTGCTATTAGTGACCCTAGCAATACCGA
GATAGAAGAAGAGTTTGGCTTTGCAACCAACCTTTCCATTGAATTGGTCTTGTGCGAAGAAAAGCAATTACGAGCCTGTA
TTAAACGTCTGTATGGCAACTTAATTGATAGCGATGATGGTGCAGGTAGAGACATCAACGAACAAGACTTAGCAGAGCTT
GTCACGGTCTCCGACAACGAGCATCAGCAAAATGAAGATCTAAGCAAAGATACATCGCCAGTAAGCCGTTTCATTCATCA
AGTACTCTTAGAAGCGATACGGAAGAACGCGTCAGATATTCACTTTGAACCTTACGAAGAGAGTTATCGTATTCGCATGC
GGTGCGATGGCATGCTACTCCAAACCAATTCACCGCCTTCTCAACTCAATAGGCGCTTAGCCGCTCGCTTAAAAATTCTC
GCTAAGTTAGACATTGCAGAAAGACGATTACCCCAAGACGGTCGAATCAAACTTCATTTGAACGCCAATACGTCCATCGA
TCTTCGAGTCTCGACCTTACCAACACTTTGGGGCGAAAAGGTGGTGTTGCGTTTACTCGATGGCGACAATGCCAAATTGG
ACATCAACGCTCTGGGCTATAGCGAACAACAAAAACAGATCTATCTCAATGCATTGTCTAAGCCGCAAGGCATGATCTTG
ATGACCGGCCCGACAGGCAGTGGCAAAACCATTTCACTCTACACTGGGCTAAGTAAGCTCAATAAAATAGAACGTAATAT
TTCGACCGCTGAAGATCCTGTTGAGATCAATTTCCCCGGGATCAACCAAGTGCAAATTCAGCCTAAAGTGGGTTTTACTT
TTGCTAAAGCTTTACGTGCCTTTCTACGTCAAGATCCCGATGTGGTGATGTTAGGGGAAATTCGAGATCTCGAGACGGCA
GAAATTGCCATCAAAGCCGCACAAACTGGTCATTTAGTCCTCTCAACCTTGCACACCAACTCAGCAGCAGAAACCATCAT
TCGCTTACAAAATATGGGGATCGAAGCATACAACTTGGCATCATCATTAAGCTTAGTTGTCGCTCAGCGCCTTGCAAGAC
GCCTATGCCCTCAATGCAAAACCGAACATCGGATGCCCTTCGCAATGCGACAGGCGTTAGATATCGGCCAAGACTGTCGT
ATTTATCAAGCAAACAAAGAAGGCTGTAACGATTGCAACGCAGGCTATAGCGGCCGTATTGGTATCTACGAAATGCTGCA
AATTAACGATCAACTTATAGAAGCCATCGCCAAGCGATTGAGCATGCCTGAACTGGAAGCCATTGCCGTTCAGCATGGCA
TGATTACGCTAAGACAAGCAGGTATAGATAAACTCAAATTGGGAATCACCAGCCACCAAGAACTTCAACGCGTACTATTC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8Z8J5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.1

100

0.622
  pilB Vibrio parahaemolyticus RIMD 2210633

60.606

100

0.606
  pilB Vibrio campbellii strain DS40M4

59.715

100

0.597
  pilB Acinetobacter baylyi ADP1

46.293

98.574

0.456
  pilB Acinetobacter baumannii D1279779

48.669

93.761

0.456
  pilB Legionella pneumophila strain ERS1305867

49.287

87.522

0.431
  pilF Neisseria gonorrhoeae MS11

42.705

100

0.428
  pilB/pilB1 Synechocystis sp. PCC 6803

36.126

100

0.369

Multiple sequence alignment