Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   CKV79_RS09630 Genome accession   NZ_LT906451
Coordinates   2118255..2118662 (-) Length   135 a.a.
NCBI ID   WP_028372720.1    Uniprot ID   A0A0W0VU54
Organism   Legionella lansingensis strain NCTC12830     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2113255..2123662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV79_RS09620 (SAMEA44548918_01943) letS 2114145..2116022 (+) 1878 WP_408606905.1 ATP-binding protein Regulator
  CKV79_RS14190 (SAMEA44548918_01944) letS 2115983..2116882 (+) 900 WP_408606906.1 response regulator Regulator
  CKV79_RS09625 (SAMEA44548918_01945) - 2116912..2118147 (+) 1236 WP_028372721.1 6-phosphofructokinase -
  CKV79_RS09630 (SAMEA44548918_01946) pilA2 2118255..2118662 (-) 408 WP_028372720.1 pilin Machinery gene
  CKV79_RS09635 (SAMEA44548918_01947) - 2118925..2119242 (+) 318 WP_028372719.1 BolA family transcriptional regulator -
  CKV79_RS09640 (SAMEA44548918_01948) - 2119235..2119666 (+) 432 WP_231950092.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  CKV79_RS09645 (SAMEA44548918_01949) - 2119669..2120073 (-) 405 WP_028372717.1 hypothetical protein -
  CKV79_RS09650 (SAMEA44548918_01950) - 2120246..2121664 (+) 1419 WP_028372716.1 APC family permease -
  CKV79_RS09655 (SAMEA44548918_01951) pheA 2121661..2122740 (+) 1080 WP_028372715.1 prephenate dehydratase -
  CKV79_RS09660 (SAMEA44548918_01952) kdsB 2122900..2123655 (-) 756 WP_028372714.1 3-deoxy-manno-octulosonate cytidylyltransferase -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14525.93 Da        Isoelectric Point: 9.0798

>NTDB_id=1147404 CKV79_RS09630 WP_028372720.1 2118255..2118662(-) (pilA2) [Legionella lansingensis strain NCTC12830]
MKQRGFTLIELMIVIAILGILISIAVPAYRQHIVRARVIEGLNLASQAKIAVTETAMTNRALPANQEAAGYTSPSPTVNV
KSITIGDKGVITITYTPEAGDGTILLVPSMRQNGELTWTCTEGTLPEKYRPSGCR

Nucleotide


Download         Length: 408 bp        

>NTDB_id=1147404 CKV79_RS09630 WP_028372720.1 2118255..2118662(-) (pilA2) [Legionella lansingensis strain NCTC12830]
ATGAAGCAGAGAGGGTTCACACTCATTGAACTGATGATTGTTATTGCTATTTTAGGGATTCTTATTTCTATTGCTGTGCC
TGCCTATAGACAGCATATTGTGCGCGCAAGGGTCATTGAAGGATTGAACTTGGCATCCCAGGCAAAAATTGCAGTGACAG
AAACAGCAATGACCAATCGTGCTTTACCTGCAAATCAGGAGGCTGCTGGTTATACCAGCCCTTCACCTACTGTCAACGTG
AAATCCATAACCATAGGAGATAAAGGGGTTATCACTATCACATACACACCTGAGGCAGGTGATGGCACCATCTTATTAGT
GCCATCGATGAGGCAGAATGGCGAATTAACTTGGACTTGCACTGAAGGAACTTTACCAGAAAAATATCGTCCGTCTGGTT
GTAGGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)

Pilin (bacterial filament) (Pilin)

(34-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0VU54

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

64.444

100

0.644
  pilA2 Legionella pneumophila strain ERS1305867

64.444

100

0.644
  pilA Ralstonia pseudosolanacearum GMI1000

44.099

100

0.526
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.854

100

0.481
  comP Acinetobacter baylyi ADP1

44.138

100

0.474
  pilE Neisseria gonorrhoeae MS11

36.538

100

0.422
  pilE Neisseria gonorrhoeae strain FA1090

34.839

100

0.4
  pilA Pseudomonas aeruginosa PAK

30.435

100

0.363

Multiple sequence alignment