Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   B5474_RS17340 Genome accession   NZ_LT605059
Coordinates   3622226..3622663 (-) Length   145 a.a.
NCBI ID   WP_005109337.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain NCTC7364     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3617226..3627663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5474_RS17315 (NCTC7364_03470) - 3617586..3617969 (+) 384 WP_000090000.1 RidA family protein -
  B5474_RS17320 (NCTC7364_03471) - 3618179..3618373 (+) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  B5474_RS17325 (NCTC7364_03472) bfr 3618618..3619082 (+) 465 WP_000678123.1 bacterioferritin -
  B5474_RS17330 (NCTC7364_03473) - 3619123..3620751 (-) 1629 WP_038405373.1 PglL family O-oligosaccharyltransferase -
  B5474_RS17335 (NCTC7364_03474) - 3620865..3622174 (-) 1310 Protein_3389 O-antigen ligase family protein -
  B5474_RS17340 (NCTC7364_03475) pilA 3622226..3622663 (-) 438 WP_005109337.1 pilin Machinery gene
  B5474_RS17345 (NCTC7364_03476) - 3623008..3623568 (-) 561 WP_020753495.1 TPM domain-containing protein -
  B5474_RS17350 (NCTC7364_03477) - 3623562..3624644 (-) 1083 WP_001246384.1 TPM domain-containing protein -
  B5474_RS17355 (NCTC7364_03478) - 3624666..3625256 (-) 591 WP_000846931.1 LemA family protein -
  B5474_RS17360 (NCTC7364_03479) - 3625387..3626274 (-) 888 WP_001017320.1 metal-dependent hydrolase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15254.43 Da        Isoelectric Point: 7.7725

>NTDB_id=1145164 B5474_RS17340 WP_005109337.1 3622226..3622663(-) (pilA) [Acinetobacter baumannii strain NCTC7364]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQDYIVRSKVTEGLNLASSYKTTIAENAGNGSADLTVGVPNFSKTENVDSI
TADKSTGAIAIKYTDKAKSLTLTLTPQDDGKSLSAGKVPTNQITWVCTVDKPDNAKYVPANCRGS

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1145164 B5474_RS17340 WP_005109337.1 3622226..3622663(-) (pilA) [Acinetobacter baumannii strain NCTC7364]
ATGAATGCACAAAAAGGTTTTACGTTAATTGAACTCATGATCGTTGTAGCGATTATTGGTATTTTAGCAGCGATCGCGAT
TCCTGCGTATCAGGATTATATTGTTCGCTCAAAAGTAACAGAAGGTCTTAATTTGGCTTCATCATATAAAACGACTATTG
CTGAGAACGCAGGTAACGGTTCGGCGGATCTTACAGTAGGAGTTCCTAATTTTTCTAAGACAGAAAATGTTGATTCAATT
ACAGCGGATAAAAGTACTGGAGCTATTGCAATTAAATATACTGATAAGGCTAAAAGTTTAACTTTGACATTAACCCCTCA
AGATGACGGAAAATCTTTGTCAGCTGGTAAAGTGCCAACTAATCAGATTACTTGGGTGTGTACGGTAGATAAACCAGATA
ATGCTAAATATGTACCAGCTAATTGTCGTGGCTCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

49.045

100

0.531
  comP Acinetobacter baylyi ADP1

46.875

100

0.517
  pilA2 Legionella pneumophila strain ERS1305867

49.315

100

0.497
  pilA2 Legionella pneumophila str. Paris

49.315

100

0.497
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.428
  pilA Acinetobacter baumannii strain A118

43.662

97.931

0.428
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

100

0.428
  pilA/pilA1 Eikenella corrodens VA1

39.103

100

0.421
  pilE Neisseria gonorrhoeae strain FA1090

37.107

100

0.407
  pilA Haemophilus influenzae 86-028NP

36.184

100

0.379

Multiple sequence alignment