Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PCPL58_RS06100 Genome accession   NZ_LT222319
Coordinates   796587..797804 (-) Length   405 a.a.
NCBI ID   WP_065349037.1    Uniprot ID   A0A193SJI0
Organism   Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 791587..802804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCPL58_RS32020 - 792894..793130 (-) 237 WP_331716566.1 DUF3094 family protein -
  PCPL58_RS06070 (PCPL58_0723) - 793015..793548 (-) 534 WP_065349034.1 MOSC domain-containing protein -
  PCPL58_RS06075 (PCPL58_0724) - 793545..794174 (-) 630 WP_003345130.1 DUF1780 domain-containing protein -
  PCPL58_RS06080 (PCPL58_0725) - 794300..794761 (+) 462 WP_065349035.1 hypothetical protein -
  PCPL58_RS06085 (PCPL58_0726) yacG 794887..795096 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PCPL58_RS06090 (PCPL58_0727) coaE 795093..795716 (-) 624 WP_058397527.1 dephospho-CoA kinase -
  PCPL58_RS06095 (PCPL58_0728) pilD 795713..796585 (-) 873 WP_065349036.1 prepilin peptidase Machinery gene
  PCPL58_RS06100 (PCPL58_0729) pilC 796587..797804 (-) 1218 WP_065349037.1 type II secretion system F family protein Machinery gene
  PCPL58_RS06105 (PCPL58_0730) pilB 797807..799501 (-) 1695 WP_046719961.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCPL58_RS29170 - 799727..800122 (+) 396 WP_083188329.1 pilin -
  PCPL58_RS06110 (PCPL58_0731) - 800285..801589 (+) 1305 WP_065349038.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44212.12 Da        Isoelectric Point: 10.1599

>NTDB_id=1143643 PCPL58_RS06100 WP_065349037.1 796587..797804(-) (pilC) [Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf]
MASKAVKVSVYKWEGVDKKGGKLSGEVSGHNLALVKAQLRKQGINFTKVRKKPVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQAFDIIAEGAENPNMRALVGSLKQEVSAGNSFATALRHKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPTAVLIVAFIVSGILLIKVVPQFQSVFAGFNAELPAFTLMVIALSEVVQQWWLAIVVLFFISFFMFK
RTYKRSQNFRDSVDRSLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNQVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDAMLDKVATYYEDEVDNMVDNMTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1143643 PCPL58_RS06100 WP_065349037.1 796587..797804(-) (pilC) [Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf]
ATGGCCAGCAAGGCAGTTAAAGTCAGTGTTTACAAGTGGGAAGGTGTGGACAAAAAAGGCGGCAAACTCAGTGGCGAAGT
GAGCGGGCACAATCTTGCGCTCGTAAAGGCTCAACTGCGCAAGCAAGGTATCAACTTCACCAAGGTACGTAAAAAGCCCG
TTTCGATCTTTGGCAAAGGCAAGAAAATCAAGCCGCTGGACATCGCATTCTTTTCTCGGCAGATGGCGACCATGATGAAG
GCAGGTGTGCCGCTTCTGCAGGCATTTGACATCATTGCCGAGGGTGCAGAAAACCCGAACATGCGTGCACTGGTTGGCTC
ACTGAAGCAGGAGGTGTCGGCAGGGAACAGCTTTGCGACGGCTCTGCGGCATAAACCAGAATACTTTGATGATTTGTTCT
GCAACCTGGTCGATGCCGGCGAACAAGCGGGAGCGTTAGAAAGCCTGCTGGATCGCGTGGCAAGCTACAAGGAAAAAACA
GAAAAGCTTAAGGCCAAAATCAAGAAGGCCATGACTTATCCTACCGCCGTATTAATTGTCGCGTTTATCGTGTCAGGTAT
TTTGCTGATCAAGGTGGTGCCGCAGTTCCAGTCTGTTTTTGCAGGGTTCAACGCGGAATTACCAGCGTTCACATTGATGG
TCATCGCGCTTTCGGAGGTCGTTCAGCAGTGGTGGCTGGCGATAGTCGTATTGTTTTTTATCAGCTTTTTCATGTTTAAG
AGGACATACAAGCGGTCTCAAAATTTTCGTGACAGCGTCGACCGCTCTCTTCTGAAAGTACCCATTATCGGACCGCTGAT
TTTCAAGTCATCCGTCGCACGTTATGCACGCACGCTTGCGACAACCTTTGCCGCAGGGGTGCCACTGGTTGAGGCACTGG
ATTCGGTTGCCGGAGCCACCGGTAACGTCGTATTCAGGAACGCGGTCAATCAAGTCAAACAGGATGTCTCTACTGGCATG
CAGCTGAATTTCTCGATGCGATCAACAGGTGTATTTCCAAGTCTGGCGATACAAATGACAGCAATCGGAGAAGAGTCAGG
GGCGCTTGACGCCATGCTCGATAAAGTCGCGACTTACTATGAAGACGAGGTCGATAATATGGTCGACAATATGACCAGTC
TCATGGAACCGATGATCATGGCGGTGCTGGGTGTTATCGTCGGCGGCTTAGTGATTGCCATGTATCTCCCCATCTTCAAA
CTTGGAAGCGTTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A193SJI0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

72.84

100

0.728
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

58.168

99.753

0.58
  pilC Legionella pneumophila strain ERS1305867

52.02

97.778

0.509
  pilG Neisseria gonorrhoeae MS11

42.68

99.506

0.425
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

42.26

100

0.425
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Thermus thermophilus HB27

38.462

99.506

0.383

Multiple sequence alignment