Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PCPL58_RS06105 Genome accession   NZ_LT222319
Coordinates   797807..799501 (-) Length   564 a.a.
NCBI ID   WP_046719961.1    Uniprot ID   -
Organism   Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 792807..804501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCPL58_RS32020 - 792894..793130 (-) 237 WP_331716566.1 DUF3094 family protein -
  PCPL58_RS06070 (PCPL58_0723) - 793015..793548 (-) 534 WP_065349034.1 MOSC domain-containing protein -
  PCPL58_RS06075 (PCPL58_0724) - 793545..794174 (-) 630 WP_003345130.1 DUF1780 domain-containing protein -
  PCPL58_RS06080 (PCPL58_0725) - 794300..794761 (+) 462 WP_065349035.1 hypothetical protein -
  PCPL58_RS06085 (PCPL58_0726) yacG 794887..795096 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PCPL58_RS06090 (PCPL58_0727) coaE 795093..795716 (-) 624 WP_058397527.1 dephospho-CoA kinase -
  PCPL58_RS06095 (PCPL58_0728) pilD 795713..796585 (-) 873 WP_065349036.1 prepilin peptidase Machinery gene
  PCPL58_RS06100 (PCPL58_0729) pilC 796587..797804 (-) 1218 WP_065349037.1 type II secretion system F family protein Machinery gene
  PCPL58_RS06105 (PCPL58_0730) pilB 797807..799501 (-) 1695 WP_046719961.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCPL58_RS29170 - 799727..800122 (+) 396 WP_083188329.1 pilin -
  PCPL58_RS06110 (PCPL58_0731) - 800285..801589 (+) 1305 WP_065349038.1 O-antigen ligase family protein -
  PCPL58_RS06115 (PCPL58_0732) - 801701..802987 (+) 1287 WP_065349039.1 lipopolysaccharide biosynthesis protein -
  PCPL58_RS06120 (PCPL58_0733) - 803037..803582 (-) 546 WP_003371273.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62477.75 Da        Isoelectric Point: 6.0143

>NTDB_id=1143644 PCPL58_RS06105 WP_046719961.1 797807..799501(-) (pilB) [Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf]
MSDAVLTGLAKQLVLAELLTETAAQQAYQQARRDKVSLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLIRQHHALPLWRRGNKLFVGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDTESGLGELADIDLGLD
IEPASDKETSLATQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVAKPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKNKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSQCKKELDIPRETLIQEGFPEEK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1143644 PCPL58_RS06105 WP_046719961.1 797807..799501(-) (pilB) [Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf]
ATGTCTGATGCTGTCCTCACCGGTTTGGCCAAACAATTGGTCTTGGCCGAGCTACTCACCGAAACTGCTGCGCAGCAAGC
TTATCAGCAGGCGCGCCGCGACAAGGTTTCACTGGTGAGCTATCTGGTCCAGAACAAACTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCAGACCAGTTTGGCGTACCTTTCATGGACCTTGCCAGTCTGGACAAGGAAAGCCAACCCAAAGGGTTG
GTCAGTGAAAAGCTGATTCGTCAGCATCATGCACTTCCGTTGTGGCGGCGTGGTAACAAGCTGTTTGTAGGCATCTCGGA
CCCTACCAACCATCAGGCGGTCACAGACATACAGTTCAGTACCGGGCTAAACACTGAAGCGATTCTGGTTGAAGATGACA
AGCTGACCATTGCCATAGACCGTTTTTTTGACACTGAAAGTGGCCTTGGGGAACTGGCGGATATCGACCTTGGCCTGGAC
ATTGAGCCTGCAAGTGACAAGGAAACCTCCCTGGCCACTCAGAGTGATGCCGATGATGCTCCAGTGGTGCGCTTCGTCAA
CAAAATGCTGATGGACGCAATACGCCTAGGGTCATCCGACCTGCACTTTGAACCTTACGAAAAGATCTTTCGCGTGCGCC
TGCGCACCGACGGTATTTTGCATGAAGTGGCCAAACCACCGATTCATCTGGCCAACCGAATTGCTGCGCGACTGAAAGTT
ATGGCCAGCCTCGATATCTCGGAACGACGAAAACCGCAGGACGGCAGGGTCAAGCTGCGAGTGTCAAAAAACAAAGCGAT
CGACTTCCGCATGAACACACTGCCAACACTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGATCCGACCAGCGCACAGA
TGGGCATTGACGCATTGGGCTATGAACCGGACCAAAAAGCTTTGTATATGGAAGCCTTGAAACAACCCCAAGGCATGATT
CTTGTGACCGGCCCCACCGGCTCGGGTAAAACCGTTTCGCTATACACCGGCCTGAACATTCTCAACACCGTGGACATCAA
CATCTCCACTGCTGAAGACCCAGTAGAAATCAATCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGTCAGGGACTGG
ACTTTTCTCAAGCCTTGCGCGCATTTCTTCGTCAAGATCCCGACGTGATCATGGTCGGCGAGATACGAGACTTGGAAACC
GCCGAAATTGCCATCAAAGCCTCACAAACCGGTCACATGGTGTTATCTACCCTGCACACTAACAGTGCCGCAGAAACTCT
GACGCGCTTGCATCATATGGGTGTTGCAGCGTTCAACATCGCTACAGCAATCAATCTCATCATTGCCCAGCGGCTGGCCC
GCAAGCTCTGTAGCCAATGCAAGAAAGAACTCGATATACCTCGTGAAACGCTCATCCAGGAGGGGTTTCCAGAGGAGAAG
ATCGGTACGTTTAAGATCTATGGCCCAGTAGGGTGTGAGCATTGCAACGGCGGCTACAGGGGCAGGGTCGGCATTTACGA
AGTAGTCAAGAAAACGCCAGAACTGGAACGCATCATCATGGAAGAAGGCAATTCGCTGGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAATGATTTGAGGACCTCCGGGCTGTCGAAGGCCATGCAGGGCATTACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.22

100

0.592
  pilB Acinetobacter baylyi ADP1

57.651

99.645

0.574
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.56
  pilB Vibrio cholerae strain A1552

50.802

99.468

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.542

98.227

0.496
  pilB Vibrio campbellii strain DS40M4

49.291

100

0.493
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

39.046

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.469

93.44

0.369

Multiple sequence alignment