Detailed information NTG1143642

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PCPL58_RS06095
• Genome accession: NZ_LT222319
• Coordinates: 795713..796585 (-)
• Length: 290 a.a.
• NCBI ID: WP_065349036.1
• Uniprot ID: A0A193SJE1
• Organism: Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 790713..801585

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCPL58_RS06065 (PCPL58_0722)	-	791500..792798 (-)	1299	WP_003430739.1	NAD(P)/FAD-dependent oxidoreductase	-
PCPL58_RS32020	-	792894..793130 (-)	237	WP_331716566.1	DUF3094 family protein	-
PCPL58_RS06070 (PCPL58_0723)	-	793015..793548 (-)	534	WP_065349034.1	MOSC domain-containing protein	-
PCPL58_RS06075 (PCPL58_0724)	-	793545..794174 (-)	630	WP_003345130.1	DUF1780 domain-containing protein	-
PCPL58_RS06080 (PCPL58_0725)	-	794300..794761 (+)	462	WP_065349035.1	hypothetical protein	-
PCPL58_RS06085 (PCPL58_0726)	yacG	794887..795096 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
PCPL58_RS06090 (PCPL58_0727)	coaE	795093..795716 (-)	624	WP_058397527.1	dephospho-CoA kinase	-
PCPL58_RS06095 (PCPL58_0728)	pilD	795713..796585 (-)	873	WP_065349036.1	prepilin peptidase	Machinery gene
PCPL58_RS06100 (PCPL58_0729)	pilC	796587..797804 (-)	1218	WP_065349037.1	type II secretion system F family protein	Machinery gene
PCPL58_RS06105 (PCPL58_0730)	pilB	797807..799501 (-)	1695	WP_046719961.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PCPL58_RS29170	-	799727..800122 (+)	396	WP_083188329.1	pilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32001.20 Da
• Isoelectric Point: 8.1507

>NTDB_id=1143642 PCPL58_RS06095 WP_065349036.1 795713..796585(-) (pilD) [Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPIMMERDWKAQSREMLGLPAEPDQPIFNLNRPHSSCPQCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLGWGLLAMSLIDADHQLLPDSMV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=1143642 PCPL58_RS06095 WP_065349036.1 795713..796585(-) (pilD) [Pseudomonas cerasi isolate Sour cherry (Prunus cerasus) symptomatic leaf]
ATGCCCCTCCTCGACCTCCTGGCCAGCTCCCCTCTGGCCTTCGTCACCACCTGCTGCATTCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTATCGCCTGCCAATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAAATGCTCGGCC
TGCCCGCCGAACCCGATCAACCCATCTTCAATCTGAACCGCCCCCATTCCAGCTGCCCGCAATGCGCACACAAAATCCGC
CCGTGGGAGAACCTGCCGGTGATCAGCTATCTGCTGCTGCGTGGTAAATGCTCGCAGTGCAAAGCCCCCATCAGCAAACG
CTACCCATTGGTAGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTGGCCTGGCATTTCGGCTTTGGTTGGCAGGCGGCGG
CGATGCTGGTATTGGGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGCTGTTACCTGATTCTATGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCTTTCGGGCTGTTTACCTCGCTTAACGATGCGCTGTGGGGAGCGGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACTGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGATTTTGCCGTTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTCGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A193SJE1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Vibrio cholerae strain A1552	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	51.986	95.517	0.497
pilD	Acinetobacter nosocomialis M2	51.264	95.517	0.49
pilD	Neisseria gonorrhoeae MS11	51.866	92.414	0.479