Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   D5R51_RS00990 Genome accession   NZ_LS997867
Coordinates   208796..210517 (+) Length   573 a.a.
NCBI ID   WP_162891872.1    Uniprot ID   -
Organism   Vibrio paracholerae strain NCTC 30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 203796..215517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5R51_RS00970 (SAMEA104470976_00196) pilM 206089..207105 (+) 1017 WP_119090747.1 type IV pilus assembly protein PilM Machinery gene
  D5R51_RS00975 (SAMEA104470976_00197) pilN 207095..207679 (+) 585 WP_162891871.1 PilN domain-containing protein Machinery gene
  D5R51_RS00980 (SAMEA104470976_00198) pilO 207672..208262 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  D5R51_RS00985 (SAMEA104470976_00199) pilP 208252..208770 (+) 519 WP_119090749.1 pilus assembly protein PilP Machinery gene
  D5R51_RS00990 (SAMEA104470976_00200) pilQ 208796..210517 (+) 1722 WP_162891872.1 type IV pilus secretin PilQ Machinery gene
  D5R51_RS00995 (SAMEA104470976_00201) aroK 210704..211228 (+) 525 WP_000818615.1 shikimate kinase AroK -
  D5R51_RS01000 (SAMEA104470976_00202) aroB 211256..212341 (+) 1086 WP_162891873.1 3-dehydroquinate synthase -
  D5R51_RS01005 (SAMEA104470976_00203) - 212393..213850 (+) 1458 WP_057642144.1 AAA family ATPase -
  D5R51_RS01010 (SAMEA104470976_00204) - 213920..214741 (+) 822 WP_000744682.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62389.53 Da        Isoelectric Point: 4.6519

>NTDB_id=1143331 D5R51_RS00990 WP_162891872.1 208796..210517(+) (pilQ) [Vibrio paracholerae strain NCTC 30]
MRNGLKTYVAQTWLTLWVGLALCASSTVFSAEFATTNQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKAPSTQLVVTVDGEFQHDYILKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGAISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLDELGVRWGFSSSNGSFGAGGSIENNLLQTVPTTLPAADFNVQDFLNVNLAATSSNASSIAFQVAK
LGSGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVL
DLSVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKS
ELLIFVTPKVVIQ

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=1143331 D5R51_RS00990 WP_162891872.1 208796..210517(+) (pilQ) [Vibrio paracholerae strain NCTC 30]
ATGAGAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTATGGGTCGGTTTGGCACTTTGTGCTTCTTCCAC
GGTGTTTAGTGCTGAATTCGCTACTACCAACCAGTTGGAAAATATTGACTTTCGAGTCAACAAAGAGAAAGCGGCGGTCT
TAATTGTCGAATTGGCCTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAGCTGCTTAAA
ACCGATGTGGCGGATGACAAGCTGTATCTGCTCGATGTGAAGGATTTTTCAACCCCTGTTGAGAGTGTGGAAGTATTCCG
TAAAGCGCCGAGCACACAACTTGTTGTGACTGTCGATGGTGAGTTTCAGCACGATTACATCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTTAAAGCTGATGAAAAGCCTAAGCCAAAGAGTGTCTTGGAAAAAGAAGGCAAGCTTATCTCG
ATTAACTTCCAAGATATTCCTGTACGTAACGTATTGCAGTTGATTGCCGATTATAACGGTTTCAACTTAGTTGTTTCTGA
CTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTTGATAAGCGCGTCGATGGCAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGATTTACGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCGGAAGAACTCGGCGATTTAAAATCAGAAATTATCAAGATTAACTTTGCCAAAGCATCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTCAACATGTTGTCTGAGCGAGGGGCGATCAGTATTGACGAACGGACTAACT
CACTGTTGATTCGAGAGCTACCCGATAACATCGCAGTGATCCGCGAGATCATCGAATCACTCGATATTCCAGTCAAACAA
GTGCAAATTGAGGCACGAATCGTCACAGTGAAAGAAGGTAACTTGGACGAACTAGGTGTGCGCTGGGGCTTCAGCTCGAG
CAATGGTTCATTTGGTGCTGGTGGCAGTATTGAAAACAACTTATTACAGACAGTTCCTACCACATTACCTGCTGCCGATT
TCAACGTTCAAGACTTCTTGAATGTCAACTTAGCGGCCACATCAAGCAATGCCTCTTCGATTGCATTCCAAGTGGCCAAA
TTAGGTTCTGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCAAAGGCTGAAATCATCTCTAGCCCTCG
TTTGATCACTACGAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTACCTAGAATCGTCTTCCAGTGGTG
CCTCTACGGTCGCGTTTAAAAAAGCGGTATTGAGCTTGAAGGTTACGCCACAAATCACCCCAGATAACCGCTTGGTGCTG
GATTTAAGCGTGACCCAAGACCGACGGGGTGAAACGGTGAAAACGGGTACGGGTGAAGCGGTATCGATCGATACTCAGCG
CATTGGTACTCAAGTACTAGTGAATAATGGAGAAACCGTGGTGCTGGGTGGCATTTTCCAACACAGCATCAATAATTCGG
TAGATAAGGTACCACTGTTGGGGGACTTGCCTGTTTTAGGTGCGTTATTCAGACGAACCTATGAACAAATGGGCAAAAGT
GAACTGCTAATTTTTGTCACGCCGAAAGTCGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(413-571)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

94.764

100

0.948
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

94.764

100

0.948
  pilQ Vibrio campbellii strain DS40M4

75.475

100

0.763
  pilQ Legionella pneumophila strain Lp02

42.729

97.208

0.415
  pilQ Legionella pneumophila strain ERS1305867

42.729

97.208

0.415
  pilQ Acinetobacter baumannii D1279779

37.801

100

0.384
  pilQ Acinetobacter baumannii strain A118

37.801

100

0.384
  comQ Acinetobacter baylyi ADP1

39.286

97.731

0.384
  pilQ Pseudomonas aeruginosa PAK

38.768

96.335

0.373

Multiple sequence alignment