Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D5R51_RS00970 Genome accession   NZ_LS997867
Coordinates   206089..207105 (+) Length   338 a.a.
NCBI ID   WP_119090747.1    Uniprot ID   -
Organism   Vibrio paracholerae strain NCTC 30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 201089..212105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5R51_RS00955 (SAMEA104470976_00193) - 201577..202308 (-) 732 WP_000495226.1 glutathione peroxidase -
  D5R51_RS00960 (SAMEA104470976_00194) oxyR 202448..203341 (+) 894 WP_119090746.1 DNA-binding transcriptional regulator OxyR -
  D5R51_RS00965 (SAMEA104470976_00195) - 203469..205946 (-) 2478 WP_029627686.1 penicillin-binding protein 1A -
  D5R51_RS00970 (SAMEA104470976_00196) pilM 206089..207105 (+) 1017 WP_119090747.1 type IV pilus assembly protein PilM Machinery gene
  D5R51_RS00975 (SAMEA104470976_00197) pilN 207095..207679 (+) 585 WP_162891871.1 PilN domain-containing protein Machinery gene
  D5R51_RS00980 (SAMEA104470976_00198) pilO 207672..208262 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  D5R51_RS00985 (SAMEA104470976_00199) pilP 208252..208770 (+) 519 WP_119090749.1 pilus assembly protein PilP Machinery gene
  D5R51_RS00990 (SAMEA104470976_00200) pilQ 208796..210517 (+) 1722 WP_162891872.1 type IV pilus secretin PilQ Machinery gene
  D5R51_RS00995 (SAMEA104470976_00201) aroK 210704..211228 (+) 525 WP_000818615.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37613.27 Da        Isoelectric Point: 7.0268

>NTDB_id=1143327 D5R51_RS00970 WP_119090747.1 206089..207105(+) (pilM) [Vibrio paracholerae strain NCTC 30]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNNTLDYQKIVKKLKELKKGLPLFSHQVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARGSTTTFQVYATKKDVVDSRLHAFKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVSSLSHPLLQDPTQR
FIQELVDKIARQIQLFTSVHGAQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFALFKNNVAKRKRSLVDGQRFST
ATGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1143327 D5R51_RS00970 WP_119090747.1 206089..207105(+) (pilM) [Vibrio paracholerae strain NCTC 30]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCGGCAGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATAACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGGTTGCCTCTATTTAGTCATCAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCACA
TCAATCTCCGTTTCCGGTGGAGGAACTGAGTCTCGATTTCGTCAAAGTGACGGAAAAGAGCCTCGCCCGCGGCAGCACCA
CGACTTTTCAAGTGTATGCCACCAAAAAAGACGTAGTCGACAGTCGTTTGCATGCCTTTAAAAAAGCGGGTTTTGAGCCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTTGCTAGCCGTGCATATCGACGTCCCGACTGGAT
GTTGGTGGATATTGGTTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGATGTGCCAT
TAGGAACTCGCCAATTGGAATCAGAACCTAGTGCTGTGAGCTCATTGAGTCATCCGCTGTTACAAGACCCCACACAGCGT
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCATGGTGCGCAAAGCCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCAGGTTTAGAGGACGCGCTTTATCAACGTTTGTCACTGCCTTGCGAAG
TGCTTAATCCTTTTGCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCTCACTGGTTGATGGCCAGCGTTTTAGTACT
GCAACCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

96.746

100

0.967
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment