Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DQL19_RS03810 Genome accession   NZ_LS483480
Coordinates   733486..734880 (+) Length   464 a.a.
NCBI ID   WP_077643716.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC13377     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 728486..739880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL19_RS03785 (NCTC13377_00745) rsmE 728503..729240 (-) 738 WP_015701564.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DQL19_RS03790 (NCTC13377_00746) lnt 729290..730819 (-) 1530 WP_015701563.1 apolipoprotein N-acyltransferase -
  DQL19_RS03795 (NCTC13377_00747) corC 730842..731741 (-) 900 WP_005648977.1 CNNM family magnesium/cobalt transport protein CorC -
  DQL19_RS03800 (NCTC13377_00748) ampD 732365..732925 (-) 561 WP_014550480.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DQL19_RS03805 (NCTC13377_00749) pilA 733040..733489 (+) 450 WP_077643715.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DQL19_RS03810 (NCTC13377_00750) pilB 733486..734880 (+) 1395 WP_077643716.1 GspE/PulE family protein Machinery gene
  DQL19_RS03815 (NCTC13377_00751) pilC 734877..736094 (+) 1218 WP_077643717.1 type II secretion system F family protein Machinery gene
  DQL19_RS03820 (NCTC13377_00752) pilD 736091..736783 (+) 693 WP_041175037.1 A24 family peptidase Machinery gene
  DQL19_RS03825 (NCTC13377_00753) rho 736839..738101 (-) 1263 WP_015701557.1 transcription termination factor Rho -
  DQL19_RS03830 (NCTC13377_00754) metJ 738349..738666 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  DQL19_RS03835 (NCTC13377_00755) cueR 738680..739066 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  DQL19_RS03840 (NCTC13377_00756) - 739143..739349 (+) 207 WP_005686717.1 heavy-metal-associated domain-containing protein -
  DQL19_RS03845 (NCTC13377_00757) - 739435..739641 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52938.37 Da        Isoelectric Point: 5.6199

>NTDB_id=1142492 DQL19_RS03810 WP_077643716.1 733486..734880(+) (pilB) [Haemophilus influenzae strain NCTC13377]
MTSYALLHTQRVTAQNGEIFTISPDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPHQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFTELG
MTENQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKTL

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1142492 DQL19_RS03810 WP_077643716.1 733486..734880(+) (pilB) [Haemophilus influenzae strain NCTC13377]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGCCTGATTTATG
GGCGCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTACTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACACGCTCTTAGCCAACCCCAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACTGCACTTCAATGGCTTAATACACCTGATAAACACATTATGACAGCGGAAGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGTCAAGATCCCGATATCATTATGCTAGGTGAAATTCGTGATGAAGAAAGTGCAAGGATTGCATTACGTGC
CGCCCAAACAGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAGTATGAAATTGAGAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAACTTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-98)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

98.704

99.784

0.985
  pilB Haemophilus influenzae Rd KW20

96.121

100

0.961
  pilB Glaesserella parasuis strain SC1401

57.895

98.276

0.569
  pilB Vibrio cholerae strain A1552

40.038

100

0.455
  pilB Vibrio campbellii strain DS40M4

40.286

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.418
  pilB Acinetobacter baylyi ADP1

38.306

100

0.409
  pilB Acinetobacter baumannii D1279779

38.877

100

0.403
  pilF Thermus thermophilus HB27

36.032

100

0.384
  pilF Neisseria gonorrhoeae MS11

36.765

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377

Multiple sequence alignment