Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DQL19_RS03805 Genome accession   NZ_LS483480
Coordinates   733040..733489 (+) Length   149 a.a.
NCBI ID   WP_077643715.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC13377     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 728040..738489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL19_RS03785 (NCTC13377_00745) rsmE 728503..729240 (-) 738 WP_015701564.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DQL19_RS03790 (NCTC13377_00746) lnt 729290..730819 (-) 1530 WP_015701563.1 apolipoprotein N-acyltransferase -
  DQL19_RS03795 (NCTC13377_00747) corC 730842..731741 (-) 900 WP_005648977.1 CNNM family magnesium/cobalt transport protein CorC -
  DQL19_RS03800 (NCTC13377_00748) ampD 732365..732925 (-) 561 WP_014550480.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DQL19_RS03805 (NCTC13377_00749) pilA 733040..733489 (+) 450 WP_077643715.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DQL19_RS03810 (NCTC13377_00750) pilB 733486..734880 (+) 1395 WP_077643716.1 GspE/PulE family protein Machinery gene
  DQL19_RS03815 (NCTC13377_00751) pilC 734877..736094 (+) 1218 WP_077643717.1 type II secretion system F family protein Machinery gene
  DQL19_RS03820 (NCTC13377_00752) pilD 736091..736783 (+) 693 WP_041175037.1 A24 family peptidase Machinery gene
  DQL19_RS03825 (NCTC13377_00753) rho 736839..738101 (-) 1263 WP_015701557.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15467.92 Da        Isoelectric Point: 9.1085

>NTDB_id=1142491 DQL19_RS03805 WP_077643715.1 733040..733489(+) (pilA) [Haemophilus influenzae strain NCTC13377]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTGKPSSCSGGSNGIA
ADITTAKGYVKSVTTSNGAITVKGDGTLANMEYILQATGNAVAGVTWTTTCKGTDVSLFPANFCGSVKK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1142491 DQL19_RS03805 WP_077643715.1 733040..733489(+) (pilA) [Haemophilus influenzae strain NCTC13377]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCTTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCAC
CTTATAAGGCTGATGTGGAATTATGCGTTTATAGCACAGGTAAACCTTCCAGTTGCTCGGGAGGAAGCAATGGAATTGCA
GCAGATATAACGACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGTAGCAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGTCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCAAAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

91.946

100

0.919
  pilA Haemophilus influenzae Rd KW20

87.919

100

0.879
  pilA Glaesserella parasuis strain SC1401

56.376

100

0.564
  pilA Vibrio campbellii strain DS40M4

39.007

94.631

0.369
  pilA2 Legionella pneumophila str. Paris

40.441

91.275

0.369
  pilA2 Legionella pneumophila strain ERS1305867

40.441

91.275

0.369
  comP Acinetobacter baylyi ADP1

42.52

85.235

0.362

Multiple sequence alignment