Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   DQN52_RS01255 Genome accession   NZ_LS483426
Coordinates   233725..234222 (-) Length   165 a.a.
NCBI ID   WP_003788333.1    Uniprot ID   A0AAX2J184
Organism   Kingella kingae strain NCTC10529     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 228725..239222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN52_RS10875 (NCTC10529_00237) - 229750..229908 (+) 159 WP_019390092.1 hypothetical protein -
  DQN52_RS01225 (NCTC10529_00238) - 229911..230636 (+) 726 WP_003791722.1 metal ABC transporter ATP-binding protein -
  DQN52_RS01230 (NCTC10529_00239) - 230685..231077 (+) 393 WP_003788325.1 pyrimidine dimer DNA glycosylase/endonuclease V -
  DQN52_RS01235 (NCTC10529_00240) - 231114..232004 (+) 891 WP_003788327.1 metal ABC transporter permease -
  DQN52_RS01245 (NCTC10529_00242) tfpZ 232335..233066 (-) 732 WP_032827860.1 TfpX/TfpZ family type IV pilin accessory protein -
  DQN52_RS01250 (NCTC10529_00243) pilA/pilA1 233088..233549 (-) 462 WP_003788330.1 pilin Machinery gene
  DQN52_RS01255 (NCTC10529_00244) pilA/pilA1 233725..234222 (-) 498 WP_003788333.1 pilin Machinery gene
  DQN52_RS01260 (NCTC10529_00245) recJ 234583..236295 (+) 1713 WP_003788336.1 single-stranded-DNA-specific exonuclease RecJ -
  DQN52_RS01265 (NCTC10529_00246) - 236512..237246 (+) 735 WP_003788337.1 histidine phosphatase family protein -
  DQN52_RS01270 (NCTC10529_00247) - 237286..237831 (+) 546 WP_003788339.1 gamma carbonic anhydrase family protein -
  DQN52_RS01275 (NCTC10529_00248) - 237822..238871 (+) 1050 WP_003788341.1 metallophosphoesterase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 16901.35 Da        Isoelectric Point: 9.0465

>NTDB_id=1141175 DQN52_RS01255 WP_003788333.1 233725..234222(-) (pilA/pilA1) [Kingella kingae strain NCTC10529]
MKTMQKGFTLIELMIVIAIIGILAAIALPAYQDYTARAQATEGFKMTSGLQTDIGVFTADKGRLPTTSDLGSDSLAKPLA
DAAQALAGKYVTKGGVTVADKGVISITFNSNGANDGKTMKLTPSLNADNGQITKWVCAGGSGKALEAKRLPSSCQEGAAA
TNSGN

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1141175 DQN52_RS01255 WP_003788333.1 233725..234222(-) (pilA/pilA1) [Kingella kingae strain NCTC10529]
ATGAAAACTATGCAAAAAGGTTTCACATTAATCGAGTTGATGATTGTAATCGCTATTATCGGTATTTTGGCTGCGATTGC
ATTACCAGCTTACCAAGATTACACTGCACGTGCACAAGCAACTGAAGGTTTCAAAATGACTTCTGGTTTGCAAACTGACA
TCGGTGTGTTCACAGCAGACAAAGGTCGTTTGCCAACTACTTCCGACTTGGGTAGTGATAGTTTGGCTAAACCATTGGCA
GATGCAGCTCAAGCATTGGCTGGTAAATACGTGACCAAAGGTGGTGTTACAGTGGCAGACAAAGGTGTTATTAGCATTAC
CTTTAATAGCAACGGTGCTAACGATGGTAAAACTATGAAGTTGACGCCTAGCTTGAATGCAGACAATGGTCAAATCACTA
AATGGGTTTGTGCTGGCGGTTCAGGTAAAGCTTTGGAAGCTAAACGCTTGCCATCTAGCTGCCAAGAAGGCGCTGCTGCT
ACTAATTCAGGTAATTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

60.256

94.545

0.57
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.314

100

0.467
  pilE Neisseria gonorrhoeae strain FA1090

40

100

0.424
  pilE Neisseria gonorrhoeae MS11

40.462

100

0.424
  pilA2 Legionella pneumophila str. Paris

44.444

92.727

0.412
  pilA2 Legionella pneumophila strain ERS1305867

44.444

92.727

0.412
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.373

97.576

0.394
  comP Acinetobacter baylyi ADP1

40.26

93.333

0.376
  pilA Ralstonia pseudosolanacearum GMI1000

35.028

100

0.376

Multiple sequence alignment