Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   DQN52_RS01250 Genome accession   NZ_LS483426
Coordinates   233088..233549 (-) Length   153 a.a.
NCBI ID   WP_003788330.1    Uniprot ID   -
Organism   Kingella kingae strain NCTC10529     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 228088..238549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN52_RS01215 (NCTC10529_00236) - 228644..229489 (+) 846 WP_032827827.1 mechanosensitive ion channel family protein -
  DQN52_RS10875 (NCTC10529_00237) - 229750..229908 (+) 159 WP_019390092.1 hypothetical protein -
  DQN52_RS01225 (NCTC10529_00238) - 229911..230636 (+) 726 WP_003791722.1 metal ABC transporter ATP-binding protein -
  DQN52_RS01230 (NCTC10529_00239) - 230685..231077 (+) 393 WP_003788325.1 pyrimidine dimer DNA glycosylase/endonuclease V -
  DQN52_RS01235 (NCTC10529_00240) - 231114..232004 (+) 891 WP_003788327.1 metal ABC transporter permease -
  DQN52_RS01245 (NCTC10529_00242) tfpZ 232335..233066 (-) 732 WP_032827860.1 TfpX/TfpZ family type IV pilin accessory protein -
  DQN52_RS01250 (NCTC10529_00243) pilA/pilA1 233088..233549 (-) 462 WP_003788330.1 pilin Machinery gene
  DQN52_RS01255 (NCTC10529_00244) pilA/pilA1 233725..234222 (-) 498 WP_003788333.1 pilin Machinery gene
  DQN52_RS01260 (NCTC10529_00245) recJ 234583..236295 (+) 1713 WP_003788336.1 single-stranded-DNA-specific exonuclease RecJ -
  DQN52_RS01265 (NCTC10529_00246) - 236512..237246 (+) 735 WP_003788337.1 histidine phosphatase family protein -
  DQN52_RS01270 (NCTC10529_00247) - 237286..237831 (+) 546 WP_003788339.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 16241.56 Da        Isoelectric Point: 5.0771

>NTDB_id=1141174 DQN52_RS01250 WP_003788330.1 233088..233549(-) (pilA/pilA1) [Kingella kingae strain NCTC10529]
MKQNGTQTGFTLIELMIVIAIIGILAAIALPAYSNYVARAQLAEGFKLTEGLRNDIAVWAAEYKAFPDADAVSSTGYLGS
QANSLNGKYVAKHGVSIAPTTGVITVQFDAGGNAGKTLTLTPTLNKDLDEQIIYWVCSGTVEKDRLPLSCQTN

Nucleotide


Download         Length: 462 bp        

>NTDB_id=1141174 DQN52_RS01250 WP_003788330.1 233088..233549(-) (pilA/pilA1) [Kingella kingae strain NCTC10529]
ATGAAACAAAATGGTACACAAACAGGCTTCACACTCATAGAATTGATGATTGTGATTGCCATTATTGGGATTTTGGCAGC
CATTGCTTTGCCTGCGTATTCCAATTATGTGGCACGCGCTCAATTGGCAGAGGGCTTTAAGCTAACTGAAGGTTTGCGTA
ATGACATTGCGGTGTGGGCAGCAGAATATAAAGCCTTTCCCGATGCCGATGCAGTGAGCAGCACGGGGTATTTGGGCTCG
CAAGCCAATAGTTTAAACGGAAAATATGTTGCTAAACACGGGGTGAGTATTGCTCCTACCACAGGTGTGATTACAGTTCA
ATTTGATGCAGGTGGCAATGCAGGTAAAACCTTAACTTTAACGCCAACACTCAACAAAGACCTTGATGAACAAATTATTT
ATTGGGTGTGCAGTGGCACGGTGGAAAAAGATCGCTTGCCTTTGTCGTGTCAAACAAATTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

55.034

97.386

0.536
  pilA2 Legionella pneumophila strain ERS1305867

41.096

95.425

0.392
  pilA2 Legionella pneumophila str. Paris

41.096

95.425

0.392
  pilA Pseudomonas aeruginosa PAK

38.065

100

0.386
  comP Acinetobacter baylyi ADP1

38

98.039

0.373
  pilA Vibrio cholerae C6706

36.774

100

0.373
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.774

100

0.373
  pilA Vibrio cholerae strain A1552

36.774

100

0.373

Multiple sequence alignment