Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   DQN40_RS00775 Genome accession   NZ_LS483413
Coordinates   126879..127820 (+) Length   313 a.a.
NCBI ID   WP_014611930.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae subsp. equisimilis strain NCTC7136     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 121879..132820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN40_RS00765 (NCTC7136_00159) rpoC 122589..126230 (+) 3642 WP_111678923.1 DNA-directed RNA polymerase subunit beta' -
  DQN40_RS00770 (NCTC7136_00160) - 126433..126798 (+) 366 WP_003055257.1 DUF1033 family protein -
  DQN40_RS00775 (NCTC7136_00161) comGA/cglA 126879..127820 (+) 942 WP_014611930.1 competence type IV pilus ATPase ComGA Machinery gene
  DQN40_RS00780 (NCTC7136_00162) comYB 127756..128787 (+) 1032 WP_084916952.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQN40_RS00785 (NCTC7136_00163) comYC 128789..129115 (+) 327 WP_084916953.1 competence type IV pilus major pilin ComGC Machinery gene
  DQN40_RS00790 (NCTC7136_00164) comGD 129090..129518 (+) 429 WP_257638821.1 competence type IV pilus minor pilin ComGD -
  DQN40_RS00795 (NCTC7136_00165) comGE 129526..129771 (+) 246 WP_049519695.1 competence type IV pilus minor pilin ComGE -
  DQN40_RS00800 (NCTC7136_00166) comGF 129752..130192 (+) 441 WP_003061780.1 competence type IV pilus minor pilin ComGF -
  DQN40_RS00805 (NCTC7136_00167) comGG 130215..130544 (+) 330 WP_003058539.1 competence type IV pilus minor pilin ComGG -
  DQN40_RS00810 (NCTC7136_00168) comYH 130607..131560 (+) 954 WP_003060701.1 class I SAM-dependent methyltransferase Machinery gene
  DQN40_RS00815 (NCTC7136_00169) - 131619..132815 (+) 1197 WP_084916957.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35165.29 Da        Isoelectric Point: 7.5610

>NTDB_id=1140677 DQN40_RS00775 WP_014611930.1 126879..127820(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC7136]
MVQALARSILKEAEQIQAQDIYILPKENQYELLIRVGDERRLVDVYRGDRMAHLISHFKFVAGMTVGEKRRCQVGSCDYD
IGGDTLLSLRLSSVGNYRGQESLVIRLLHHQQRSLHYWFDGLKTVARQIGGRGLYLFAGPVGSGKTTLMHQLIADYYQGA
QVISIEDPVEIKNHQALQLQVNDSIGMTYDNLIKLSLRHRPDILIIGEIRDSQTARAVIRASLTGVLVFSTVHAKSISGV
YARLLELGVTRAELDNCLALVAYQRLLNGGALIDSTQTEFEHYSPNQWNQKIDDLLAAGHLSPEQARFEKIIQ

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1140677 DQN40_RS00775 WP_014611930.1 126879..127820(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC7136]
ATGGTACAAGCATTAGCAAGATCTATTTTGAAAGAAGCTGAGCAGATTCAAGCTCAAGATATCTATATTTTGCCAAAGGA
AAATCAGTATGAGCTATTGATAAGGGTAGGAGATGAAAGGAGATTGGTGGATGTTTATCGGGGCGATCGGATGGCTCATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGACCGTTGGTGAGAAACGACGCTGTCAAGTCGGTTCTTGTGATTATGAT
ATCGGTGGAGACACACTTTTGTCTCTACGCCTGTCTAGTGTTGGAAATTACCGTGGACAAGAGAGCCTAGTGATTCGGTT
ATTACATCATCAACAAAGAAGTTTACATTACTGGTTTGACGGACTGAAAACAGTTGCACGTCAGATTGGAGGCCGAGGAC
TCTATCTTTTTGCAGGTCCAGTCGGATCAGGCAAAACGACTTTGATGCACCAATTGATTGCTGATTATTATCAAGGAGCA
CAGGTCATTAGCATAGAAGATCCGGTGGAAATTAAAAATCATCAAGCGCTCCAATTACAAGTTAATGATAGTATTGGCAT
GACTTACGACAACTTGATCAAACTATCTTTACGGCATCGCCCTGATATTTTAATCATTGGTGAAATTCGAGACAGCCAGA
CAGCTAGAGCGGTTATTAGAGCAAGTTTAACAGGTGTCTTAGTATTTTCAACAGTTCATGCCAAAAGCATTTCTGGTGTT
TATGCCAGATTACTAGAACTAGGAGTGACAAGAGCAGAATTAGACAATTGTTTGGCTTTGGTTGCTTACCAACGATTGCT
TAATGGAGGAGCATTGATTGACTCAACTCAAACAGAATTTGAACACTACTCACCAAACCAATGGAATCAGAAAATTGATG
ACCTTCTTGCAGCGGGACATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.935

99.042

0.613
  comYA Streptococcus mutans UA140

61.415

99.361

0.61
  comYA Streptococcus mutans UA159

61.415

99.361

0.61
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.361

0.604
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.486

99.361

0.591
  comGA Lactococcus lactis subsp. cremoris KW2

52.412

99.361

0.521
  comGA Latilactobacillus sakei subsp. sakei 23K

38.908

93.61

0.364

Multiple sequence alignment