Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DQN24_RS03295 Genome accession   NZ_LS483411
Coordinates   653935..655329 (-) Length   464 a.a.
NCBI ID   WP_111695393.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC11426     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 648935..660329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN24_RS03260 (NCTC11426_00645) - 649185..649391 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  DQN24_RS03265 (NCTC11426_00646) - 649465..649671 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  DQN24_RS03270 (NCTC11426_00647) cueR 649748..650134 (+) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  DQN24_RS03275 (NCTC11426_00648) metJ 650148..650465 (-) 318 WP_021035707.1 met regulon transcriptional regulator MetJ -
  DQN24_RS03280 (NCTC11426_00649) rho 650713..651975 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  DQN24_RS03285 (NCTC11426_00650) pilD 652029..652721 (-) 693 WP_041175383.1 prepilin peptidase Machinery gene
  DQN24_RS03290 (NCTC11426_00651) pilC 652718..653938 (-) 1221 WP_021035705.1 type II secretion system F family protein Machinery gene
  DQN24_RS03295 (NCTC11426_00652) pilB 653935..655329 (-) 1395 WP_111695393.1 GspE/PulE family protein Machinery gene
  DQN24_RS03300 (NCTC11426_00653) pilA 655326..655775 (-) 450 WP_021035703.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DQN24_RS03305 (NCTC11426_00654) ampD 655890..656444 (+) 555 WP_021035702.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DQN24_RS03310 (NCTC11426_00655) corC 657076..657975 (+) 900 WP_021035700.1 CNNM family magnesium/cobalt transport protein CorC -
  DQN24_RS03315 (NCTC11426_00656) lnt 657998..659527 (+) 1530 WP_111695739.1 apolipoprotein N-acyltransferase -
  DQN24_RS03320 (NCTC11426_00657) rsmE 659577..660314 (+) 738 WP_111695394.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52911.37 Da        Isoelectric Point: 5.6520

>NTDB_id=1140664 DQN24_RS03295 WP_111695393.1 653935..655329(-) (pilB) [Haemophilus influenzae strain NCTC11426]
MTSYALLHTQRVIAQNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
TFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCNQGYQGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVNQGMTDNKELERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1140664 DQN24_RS03295 WP_111695393.1 653935..655329(-) (pilB) [Haemophilus influenzae strain NCTC11426]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAGGTATTTACGATCTCGCCAGATTTATG
GGAACGTAATCAGCAACAACAGTCTCTGCTCTTACGTTATTTTGCGTTACCACTCAAAGAAGAAAATAATCGTCTTTGGT
TAGGTGTCGATTCCCTTTCTAATCTTTCCGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGTCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTGGATGGCATTATTCAAAGCCAAATTAACCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
ACTTTCTTACGCCAAGATCCCGATATTATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACCGGGCATTTAGTACTCTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAACGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCAATCAAGGTTATCAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAATCAAGGAATGACGGATAATA
AAGAACTTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.336

100

0.963
  pilB Haemophilus influenzae Rd KW20

96.328

99.784

0.961
  pilB Glaesserella parasuis strain SC1401

57.018

98.276

0.56
  pilB Vibrio cholerae strain A1552

39.469

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Legionella pneumophila strain ERS1305867

39.148

100

0.416
  pilB Acinetobacter baylyi ADP1

37.903

100

0.405
  pilB Acinetobacter baumannii D1279779

38.669

100

0.401
  pilF Neisseria gonorrhoeae MS11

36.345

100

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.966

100

0.373
  pilF Thermus thermophilus HB27

35.611

100

0.371

Multiple sequence alignment