Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   DQM53_RS00745 Genome accession   NZ_LS483361
Coordinates   124989..125930 (+) Length   313 a.a.
NCBI ID   WP_111714768.1    Uniprot ID   A0A9X8XER5
Organism   Streptococcus dysgalactiae subsp. equisimilis strain NCTC6179     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 119989..130930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM53_RS00735 (NCTC6179_00150) rpoC 120699..124340 (+) 3642 WP_111714766.1 DNA-directed RNA polymerase subunit beta' -
  DQM53_RS00740 (NCTC6179_00151) - 124543..124908 (+) 366 WP_003055257.1 DUF1033 family protein -
  DQM53_RS00745 (NCTC6179_00152) comGA/cglA 124989..125930 (+) 942 WP_111714768.1 competence type IV pilus ATPase ComGA Machinery gene
  DQM53_RS00750 (NCTC6179_00153) comYB 125866..126897 (+) 1032 WP_111717873.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQM53_RS00755 (NCTC6179_00154) comYC 126899..127225 (+) 327 WP_111714771.1 competence type IV pilus major pilin ComGC Machinery gene
  DQM53_RS00760 (NCTC6179_00155) comGD 127200..127628 (+) 429 WP_111714773.1 competence type IV pilus minor pilin ComGD -
  DQM53_RS00765 (NCTC6179_00156) comGE 127636..127881 (+) 246 WP_231871990.1 competence type IV pilus minor pilin ComGE -
  DQM53_RS00770 (NCTC6179_00157) comGF 127862..128302 (+) 441 WP_111714778.1 competence type IV pilus minor pilin ComGF -
  DQM53_RS00775 (NCTC6179_00158) comGG 128325..128654 (+) 330 WP_231872024.1 competence type IV pilus minor pilin ComGG -
  DQM53_RS00780 (NCTC6179_00159) comYH 128717..129670 (+) 954 WP_111714783.1 class I SAM-dependent methyltransferase Machinery gene
  DQM53_RS00785 (NCTC6179_00160) - 129729..130925 (+) 1197 WP_111714785.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35207.37 Da        Isoelectric Point: 7.3465

>NTDB_id=1138498 DQM53_RS00745 WP_111714768.1 124989..125930(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC6179]
MVQALAKSILKEAEQIQAQDIYILPKEDQYELLIRVGDERRLVDVYRGDRMAHLISHFKFVAGMTVGEKRRCQVGSCDYD
IGEDTLLSLRLSSVGNYRGQESLVIRLLHHQQRSLHYWFDGLKTVARQIGGRGLYLFAGPVGSGKTTLMHQLIADYYQGA
QVISIEDPVEIKNHQALQLQVNDSIGMTYDNLIKLSLRHRPDILIIGEIRDSHTARAVIRASLTGVLVFSTVHAKSISGV
YARLLELGVTRAELDNCLALVAYQRLLNGGALIDSTQTEFEHYSPNQWNQKIDHLLAAGHLSPEQARLEKIIQ

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1138498 DQM53_RS00745 WP_111714768.1 124989..125930(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC6179]
ATGGTACAAGCATTAGCAAAATCTATTTTGAAAGAAGCTGAGCAGATTCAAGCTCAAGATATCTATATTTTGCCAAAGGA
AGATCAGTATGAGCTATTGATAAGGGTAGGAGATGAAAGGAGATTGGTGGATGTTTATCGGGGCGATCGGATGGCTCATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGACCGTTGGTGAGAAACGACGCTGTCAAGTCGGTTCTTGTGATTATGAT
ATCGGTGAAGACACACTTTTGTCTCTACGCCTGTCTAGTGTTGGAAATTACCGTGGACAAGAGAGCCTAGTGATTCGGTT
ATTACATCATCAACAAAGAAGTTTACATTACTGGTTTGACGGACTGAAAACAGTTGCACGTCAGATTGGAGGCCGAGGAC
TCTATCTTTTTGCAGGTCCAGTCGGATCAGGCAAAACGACTTTGATGCACCAATTGATTGCTGATTATTATCAGGGAGCA
CAGGTCATTAGCATAGAAGATCCGGTGGAAATTAAAAATCATCAAGCGCTCCAATTACAAGTTAATGATAGTATTGGCAT
GACTTACGACAACTTGATCAAACTATCTTTACGGCATCGCCCTGATATTTTAATCATTGGTGAAATTCGAGACAGCCACA
CAGCTAGAGCGGTTATTAGAGCAAGTTTAACAGGTGTCTTAGTATTTTCAACAGTTCATGCCAAAAGCATTTCTGGTGTT
TATGCCAGATTACTAGAACTAGGAGTGACAAGAGCAGAATTAGACAATTGTTTGGCTTTGGTTGCTTACCAACGATTGCT
TAATGGAGGAGCATTGATTGACTCAACTCAAACAGAATTTGAACACTACTCACCAAACCAATGGAATCAGAAAATTGATC
ACCTTCTTGCAGCAGGACATCTCAGTCCGGAGCAAGCAAGGCTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.258

99.042

0.617
  comYA Streptococcus mutans UA140

61.093

99.361

0.607
  comYA Streptococcus mutans UA159

61.093

99.361

0.607
  comYA Streptococcus gordonii str. Challis substr. CH1

61.093

99.361

0.607
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

60.129

99.361

0.597
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.807

99.361

0.594
  comGA Lactococcus lactis subsp. cremoris KW2

51.447

99.361

0.511
  comGA Latilactobacillus sakei subsp. sakei 23K

38.908

93.61

0.364

Multiple sequence alignment