Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   DQL13_RS00710 Genome accession   NZ_LS483354
Coordinates   122139..123077 (+) Length   312 a.a.
NCBI ID   WP_012514780.1    Uniprot ID   B4U054
Organism   Streptococcus equi subsp. zooepidemicus strain NCTC11606     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 117139..128077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL13_RS00685 (NCTC11606_00138) - 117303..118349 (+) 1047 WP_043030344.1 acyltransferase family protein -
  DQL13_RS00690 (NCTC11606_00139) - 118410..118775 (+) 366 WP_012514777.1 DUF1033 family protein -
  DQL13_RS00695 - 119052..119243 (+) 192 Protein_104 lactococcin 972 family bacteriocin -
  DQL13_RS00700 (NCTC11606_00140) - 119308..121311 (+) 2004 WP_111721612.1 DUF1430 domain-containing protein -
  DQL13_RS00705 (NCTC11606_00141) - 121313..121981 (+) 669 WP_012514779.1 ATP-binding cassette domain-containing protein -
  DQL13_RS00710 (NCTC11606_00142) comGA/cglA 122139..123077 (+) 939 WP_012514780.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL13_RS00715 (NCTC11606_00143) comYB 123010..124044 (+) 1035 WP_228275455.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL13_RS00720 (NCTC11606_00144) comGC/cglC 124045..124368 (+) 324 WP_012514782.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL13_RS00725 (NCTC11606_00145) comGD 124346..124771 (+) 426 WP_012514783.1 competence type IV pilus minor pilin ComGD -
  DQL13_RS00730 (NCTC11606_00146) comGE 124776..125018 (+) 243 WP_012677236.1 competence type IV pilus minor pilin ComGE -
  DQL13_RS00735 (NCTC11606_00147) comYF 125005..125439 (+) 435 WP_037580631.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL13_RS00740 (NCTC11606_00148) comGG 125417..125779 (+) 363 WP_012514785.1 competence type IV pilus minor pilin ComGG -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35226.49 Da        Isoelectric Point: 7.1374

>NTDB_id=1138135 DQL13_RS00710 WP_012514780.1 122139..123077(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain NCTC11606]
MVQELAKRLIRQAEELQAQDIYILPRGAGYELLMRIGDDRRLIDVCESDRMANLISHFKFVAGMNVGEKRRCQLGACDYD
LDGTKVSLRLSAVGDYQGQESLVIRLLYHHQRQLRYWFDGLERVTSAIGARGLYLFSGPVGSGKTTLMYQLVSDYCKELQ
VISIEDPVEIKNDQLLQLQVNDSIGMTYDNLIKLSLRHRPDVLIIGEIRDTQTARAVIRASLTGAMVFSTVHAKSISGVY
ARLLELGISRAELDNSLAMVAYQRFISGGALIDCAQKEFEHHQASQWNQKIDQLLAEGHLNTKQARLEKIIQ

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1138135 DQL13_RS00710 WP_012514780.1 122139..123077(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain NCTC11606]
ATGGTTCAAGAATTAGCCAAGAGGCTTATCAGGCAGGCCGAAGAATTGCAGGCTCAGGATATTTATATACTGCCAAGAGG
GGCAGGCTATGAGCTTTTGATGAGGATAGGTGATGACAGGAGGCTAATAGATGTTTGTGAAAGTGATCGGATGGCTAATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGAATGTGGGAGAAAAAAGACGCTGCCAGCTCGGTGCTTGTGACTACGAC
CTTGATGGCACTAAGGTATCTCTACGATTATCAGCTGTGGGTGACTATCAGGGACAGGAGAGCTTGGTTATTCGTCTCTT
GTATCATCATCAACGCCAGCTCAGGTATTGGTTTGATGGTTTAGAGCGAGTCACATCAGCTATTGGAGCCAGAGGCCTTT
ACCTCTTTTCTGGACCGGTTGGATCAGGTAAGACAACCCTGATGTATCAGCTAGTCTCTGACTATTGCAAGGAGCTTCAG
GTGATCAGTATTGAGGATCCTGTGGAAATAAAAAATGACCAGCTCCTGCAATTACAGGTTAACGACAGCATTGGCATGAC
ATACGATAATCTGATTAAGCTCTCCTTGCGTCATCGTCCAGATGTGTTGATTATCGGAGAAATTAGAGATACACAGACTG
CTAGAGCGGTTATTAGGGCTAGCCTGACAGGAGCAATGGTCTTTTCAACCGTCCATGCAAAGAGTATTTCAGGTGTTTAC
GCTAGGCTGTTAGAATTAGGCATTTCTAGGGCAGAATTGGATAATAGTCTAGCAATGGTTGCTTACCAGCGATTTATTAG
TGGAGGTGCTTTAATTGATTGTGCGCAAAAGGAATTTGAACATCATCAAGCCAGCCAGTGGAATCAGAAGATTGATCAGC
TTCTTGCAGAGGGACATCTCAATACCAAGCAGGCAAGGCTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B4U054

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

69.032

99.359

0.686
  comYA Streptococcus mutans UA140

63.023

99.679

0.628
  comYA Streptococcus mutans UA159

63.023

99.679

0.628
  comYA Streptococcus gordonii str. Challis substr. CH1

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae D39

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae R6

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

60.968

99.359

0.606
  comGA Lactococcus lactis subsp. cremoris KW2

53.376

99.679

0.532
  comGA Latilactobacillus sakei subsp. sakei 23K

39.597

95.513

0.378

Multiple sequence alignment