Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   DQL83_RS04735 Genome accession   NZ_LS483311
Coordinates   901854..902282 (+) Length   142 a.a.
NCBI ID   WP_000934385.1    Uniprot ID   A0A2K0CLZ8
Organism   Staphylococcus aureus strain NCTC6136     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 892851..937013 901854..902282 within 0


Gene organization within MGE regions


Location: 892851..937013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL83_RS04655 (NCTC6136_00861) sufB 892851..894248 (+) 1398 WP_001074405.1 Fe-S cluster assembly protein SufB -
  DQL83_RS04660 (NCTC6136_00862) - 894316..895365 (-) 1050 WP_031836904.1 tyrosine-type recombinase/integrase -
  DQL83_RS04665 (NCTC6136_00863) - 895478..895678 (+) 201 WP_000143212.1 excisionase -
  DQL83_RS04670 (NCTC6136_00864) - 895615..896520 (-) 906 WP_000391592.1 hypothetical protein -
  DQL83_RS04675 (NCTC6136_00865) - 896552..897277 (-) 726 WP_031836905.1 PH domain-containing protein -
  DQL83_RS04680 (NCTC6136_00866) - 897305..897979 (-) 675 WP_000775187.1 ImmA/IrrE family metallo-endopeptidase -
  DQL83_RS04685 (NCTC6136_00867) - 897996..898328 (-) 333 WP_001055143.1 helix-turn-helix domain-containing protein -
  DQL83_RS04690 (NCTC6136_00868) - 898591..898785 (+) 195 WP_000108122.1 helix-turn-helix domain-containing protein -
  DQL83_RS04695 (NCTC6136_00869) - 898785..899552 (+) 768 WP_001002757.1 phage antirepressor Ant -
  DQL83_RS04700 (NCTC6136_00870) tscA 899553..899777 (+) 225 WP_000187184.1 type II toxin-antitoxin system antitoxin TscA -
  DQL83_RS04705 (NCTC6136_00871) - 899817..900266 (+) 450 WP_001094943.1 hypothetical protein -
  DQL83_RS04710 (NCTC6136_00872) - 900280..900501 (+) 222 WP_000977378.1 hypothetical protein -
  DQL83_RS04715 (NCTC6136_00873) - 900494..900655 (+) 162 WP_000066017.1 DUF1270 domain-containing protein -
  DQL83_RS04720 (NCTC6136_00874) - 900747..901007 (+) 261 WP_000291090.1 DUF1108 family protein -
  DQL83_RS04725 (NCTC6136_00875) - 901017..901238 (+) 222 WP_000815401.1 DUF2483 family protein -
  DQL83_RS04730 (NCTC6136_00876) - 901231..901854 (+) 624 WP_000139720.1 DUF1071 domain-containing protein -
  DQL83_RS04735 (NCTC6136_00877) ssbA 901854..902282 (+) 429 WP_000934385.1 single-stranded DNA-binding protein Machinery gene
  DQL83_RS04740 (NCTC6136_00878) - 902296..902970 (+) 675 WP_031836907.1 putative HNHc nuclease -
  DQL83_RS15075 (NCTC6136_00879) - 902967..903116 (+) 150 WP_001081076.1 hypothetical protein -
  DQL83_RS04745 (NCTC6136_00880) - 903109..903390 (-) 282 WP_000414755.1 hypothetical protein -
  DQL83_RS04750 (NCTC6136_00881) - 903456..904226 (+) 771 WP_000190253.1 conserved phage C-terminal domain-containing protein -
  DQL83_RS04755 (NCTC6136_00882) - 904236..905015 (+) 780 WP_031836908.1 ATP-binding protein -
  DQL83_RS04760 (NCTC6136_00883) - 905009..905167 (+) 159 WP_031836909.1 hypothetical protein -
  DQL83_RS04765 (NCTC6136_00884) - 905180..905401 (+) 222 WP_001123674.1 DUF3269 family protein -
  DQL83_RS04770 (NCTC6136_00885) - 905411..905818 (+) 408 WP_031836910.1 RusA family crossover junction endodeoxyribonuclease -
  DQL83_RS04775 (NCTC6136_00886) - 905818..906003 (+) 186 WP_031836911.1 DUF3113 family protein -
  DQL83_RS04780 (NCTC6136_00887) - 906004..906366 (+) 363 WP_000536051.1 SA1788 family PVL leukocidin-associated protein -
  DQL83_RS04785 (NCTC6136_00888) - 906366..906620 (+) 255 WP_000022736.1 DUF3310 domain-containing protein -
  DQL83_RS04790 (NCTC6136_00889) - 906620..906868 (+) 249 WP_060474161.1 SAV1978 family virulence-associated passenger protein -
  DQL83_RS04795 (NCTC6136_00890) - 906882..907088 (+) 207 WP_000693989.1 hypothetical protein -
  DQL83_RS04800 (NCTC6136_00891) - 907091..907495 (+) 405 WP_031836912.1 hypothetical protein -
  DQL83_RS04805 (NCTC6136_00892) - 907492..907686 (+) 195 WP_000983953.1 hypothetical protein -
  DQL83_RS04810 (NCTC6136_00893) - 907683..908135 (+) 453 WP_000982706.1 YopX family protein -
  DQL83_RS04815 (NCTC6136_00894) - 908132..908635 (+) 504 WP_031928724.1 hypothetical protein -
  DQL83_RS04820 (NCTC6136_00895) - 908638..908835 (+) 198 WP_094321643.1 hypothetical protein -
  DQL83_RS04825 (NCTC6136_00896) - 908828..909076 (+) 249 WP_031836882.1 DUF1024 family protein -
  DQL83_RS04830 (NCTC6136_00897) - 909069..909602 (+) 534 WP_031836883.1 dUTP diphosphatase -
  DQL83_RS04835 (NCTC6136_00898) - 909639..909923 (+) 285 WP_223289268.1 hypothetical protein -
  DQL83_RS04840 (NCTC6136_00899) - 909972..910496 (+) 525 WP_031836885.1 hypothetical protein -
  DQL83_RS04845 (NCTC6136_00900) rinB 910543..910716 (+) 174 WP_000595259.1 transcriptional activator RinB -
  DQL83_RS04850 (NCTC6136_00901) - 910717..911118 (+) 402 WP_000286968.1 hypothetical protein -
  DQL83_RS04855 (NCTC6136_00902) - 911471..911965 (+) 495 WP_000594088.1 terminase small subunit -
  DQL83_RS04860 (NCTC6136_00903) - 911958..913181 (+) 1224 WP_001037585.1 PBSX family phage terminase large subunit -
  DQL83_RS04865 (NCTC6136_00904) - 913178..914602 (+) 1425 WP_000177426.1 phage portal protein -
  DQL83_RS04870 (NCTC6136_00905) - 914571..915521 (+) 951 WP_000184132.1 phage head morphogenesis protein -
  DQL83_RS04875 (NCTC6136_00906) - 915617..916201 (+) 585 WP_001019219.1 DUF4355 domain-containing protein -
  DQL83_RS04880 (NCTC6136_00907) - 916218..917132 (+) 915 WP_000235168.1 phage major capsid protein -
  DQL83_RS15080 (NCTC6136_00908) - 917144..917287 (+) 144 WP_000002931.1 hypothetical protein -
  DQL83_RS04885 (NCTC6136_00909) - 917293..917643 (+) 351 WP_000177348.1 phage head-tail adapter protein -
  DQL83_RS04890 (NCTC6136_00910) - 917655..917990 (+) 336 WP_000483041.1 phage head closure protein -
  DQL83_RS04895 (NCTC6136_00911) - 917977..918384 (+) 408 WP_001151323.1 HK97-gp10 family putative phage morphogenesis protein -
  DQL83_RS04900 (NCTC6136_00912) - 918397..918822 (+) 426 WP_000270190.1 DUF3168 domain-containing protein -
  DQL83_RS04905 (NCTC6136_00913) - 918823..919380 (+) 558 WP_000057583.1 hypothetical protein -
  DQL83_RS04910 (NCTC6136_00914) - 919447..919953 (+) 507 WP_000134337.1 tail assembly chaperone -
  DQL83_RS15085 (NCTC6136_00915) - 920133..920282 (+) 150 WP_000617400.1 hypothetical protein -
  DQL83_RS04915 (NCTC6136_00916) - 920286..923171 (+) 2886 WP_000379446.1 hypothetical protein -
  DQL83_RS04920 (NCTC6136_00917) - 923186..924127 (+) 942 WP_000560181.1 phage tail domain-containing protein -
  DQL83_RS04925 (NCTC6136_00918) - 924138..926024 (+) 1887 WP_029549389.1 SGNH/GDSL hydrolase family protein -
  DQL83_RS04930 (NCTC6136_00919) - 926037..927935 (+) 1899 WP_000323240.1 hypothetical protein -
  DQL83_RS04935 (NCTC6136_00920) - 927935..929758 (+) 1824 WP_000259635.1 phage baseplate upper protein -
  DQL83_RS04940 (NCTC6136_00921) - 929758..930135 (+) 378 WP_000705915.1 DUF2977 domain-containing protein -
  DQL83_RS04945 (NCTC6136_00922) - 930139..930312 (+) 174 WP_001790193.1 XkdX family protein -
  DQL83_RS04950 (NCTC6136_00923) - 930353..930652 (+) 300 WP_000466769.1 DUF2951 domain-containing protein -
  DQL83_RS04955 (NCTC6136_00924) - 930789..932663 (+) 1875 WP_000524050.1 glucosaminidase domain-containing protein -
  DQL83_RS04960 (NCTC6136_00925) - 932676..933914 (+) 1239 WP_000276657.1 BppU family phage baseplate upper protein -
  DQL83_RS04965 (NCTC6136_00926) - 933920..934315 (+) 396 WP_000398858.1 hypothetical protein -
  DQL83_RS04970 (NCTC6136_00927) - 934371..934646 (+) 276 WP_000351119.1 phage holin -
  DQL83_RS04975 (NCTC6136_00928) - 934633..936045 (+) 1413 WP_111724406.1 N-acetylmuramoyl-L-alanine amidase -
  DQL83_RS04980 (NCTC6136_00929) eta 936171..937013 (+) 843 WP_001065781.1 exfoliative toxin A -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 15872.58 Da        Isoelectric Point: 8.4825

>NTDB_id=1135896 DQL83_RS04735 WP_000934385.1 901854..902282(+) (ssbA) [Staphylococcus aureus strain NCTC6136]
MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGEREADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTR
SYENKVGQRVFVTEVVADSVQFLEPKNNNQQPNNNYHQQRQTQTGNNPFDNTTAITDDDLPF

Nucleotide


Download         Length: 429 bp        

>NTDB_id=1135896 DQL83_RS04735 WP_000934385.1 901854..902282(+) (ssbA) [Staphylococcus aureus strain NCTC6136]
ATGTTAAACAGAGCAGTATTAGTAGGACGCTTAACAAAAGACCCAGAATTAAGAAGCGCGCCAAATGGCGTAAATGTAGG
TACATTCACATTGGCAGTAAACAGAACATTCACGAATGCTCAAGGCGAGCGTGAAGCAGATTTTATAAACGTAGTAGTGT
TCAAGAAACAAGCTGAAAATGTTAAAAACTACCTTTCTAAAGGGTCGCTGGCAGGTGTAGACGGGCGACTACAAACACGT
AGCTACGAAAATAAAGTCGGGCAACGTGTATTTGTGACAGAAGTAGTAGCGGACAGTGTTCAATTCTTAGAACCGAAGAA
TAACAACCAACAACCAAACAACAATTATCATCAACAAAGACAAACTCAAACTGGTAATAATCCTTTTGATAATACCACTG
CGATTACTGATGATGACTTACCGTTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2K0CLZ8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

58.14

100

0.704

  ssb Latilactobacillus sakei subsp. sakei 23K

50.588

100

0.606

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

74.648

0.437

  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.958

100

0.423

  ssbA Streptococcus mutans UA159

40.845

100

0.408

  ssbB/cilA Streptococcus mitis NCTC 12261

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae Rx1

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae D39

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae R6

39.437

100

0.394

  ssbB/cilA Streptococcus mitis SK321

39.437

100

0.394


Multiple sequence alignment