Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   STHERMO_RS08675 Genome accession   NZ_LR822006
Coordinates   1666800..1667741 (-) Length   313 a.a.
NCBI ID   WP_011226629.1    Uniprot ID   -
Organism   Streptococcus thermophilus isolate STH_CIRM_16     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1661800..1672741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STHERMO_RS08635 (STHERMO_1935) - 1661876..1663069 (-) 1194 WP_022096898.1 acetate kinase -
  STHERMO_RS08640 (STHERMO_1936) comYH 1663125..1664081 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  STHERMO_RS08645 (STHERMO_1937) comGG 1664126..1664443 (-) 318 WP_002952035.1 competence type IV pilus minor pilin ComGG -
  STHERMO_RS08650 (STHERMO_1938) comYF 1664421..1664858 (-) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  STHERMO_RS08655 (STHERMO_1939) comGE 1664842..1665135 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  STHERMO_RS08660 (STHERMO_1940) comYD 1665107..1665535 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  STHERMO_RS08665 (STHERMO_1941) comYC 1665495..1665821 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  STHERMO_RS08670 (STHERMO_1942) comYB 1665818..1666918 (-) 1101 WP_148148900.1 competence type IV pilus assembly protein ComGB Machinery gene
  STHERMO_RS08675 (STHERMO_1943) comGA/cglA/cilD 1666800..1667741 (-) 942 WP_011226629.1 competence type IV pilus ATPase ComGA Machinery gene
  STHERMO_RS08680 (STHERMO_1944) - 1667822..1668184 (-) 363 WP_011226630.1 DUF1033 family protein -
  STHERMO_RS08685 (STHERMO_1945) rpoC 1668343..1671981 (-) 3639 WP_011226631.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35422.56 Da        Isoelectric Point: 6.3927

>NTDB_id=1130467 STHERMO_RS08675 WP_011226629.1 1666800..1667741(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_16]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMNYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1130467 STHERMO_RS08675 WP_011226629.1 1666800..1667741(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_16]
ATGGTAACAGAATTTGCTAAGGAAATGATAAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTTTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCTATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GAACTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGTGTAACTGTTCTCTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCTACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.273

99.361

0.649
  comYA Streptococcus mutans UA159

64.537

100

0.645
  comYA Streptococcus mutans UA140

64.537

100

0.645
  comGA/cglA/cilD Streptococcus pneumoniae D39

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae R6

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

63.987

99.361

0.636
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.218

99.681

0.61
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

38.816

97.125

0.377

Multiple sequence alignment