Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSYRH_RS21845 Genome accession   NZ_CP006256
Coordinates   5068396..5069613 (+) Length   405 a.a.
NCBI ID   WP_046719962.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae HS191     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5063396..5074613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYRH_RS21830 (PsyrH_22305) - 5064611..5065915 (-) 1305 WP_046720349.1 O-antigen ligase family protein -
  PSYRH_RS27325 - 5066078..5066473 (-) 396 WP_046719960.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PSYRH_RS21840 (PsyrH_22310) pilB 5066699..5068393 (+) 1695 WP_046719961.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSYRH_RS21845 (PsyrH_22315) pilC 5068396..5069613 (+) 1218 WP_046719962.1 type II secretion system F family protein Machinery gene
  PSYRH_RS21850 (PsyrH_22320) pilD 5069615..5070508 (+) 894 WP_046719963.1 A24 family peptidase Machinery gene
  PSYRH_RS21855 (PsyrH_22325) coaE 5070505..5071128 (+) 624 WP_046719964.1 dephospho-CoA kinase -
  PSYRH_RS21860 (PsyrH_22330) yacG 5071125..5071334 (+) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PSYRH_RS21865 (PsyrH_22335) - 5071460..5071921 (-) 462 WP_046720350.1 hypothetical protein -
  PSYRH_RS21870 (PsyrH_22340) - 5072049..5072678 (+) 630 WP_003430735.1 DUF1780 domain-containing protein -
  PSYRH_RS21875 (PsyrH_22345) - 5072675..5073208 (+) 534 WP_003317783.1 MOSC domain-containing protein -
  PSYRH_RS29100 - 5073093..5073329 (+) 237 WP_223886507.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44252.14 Da        Isoelectric Point: 10.1095

>NTDB_id=113035 PSYRH_RS21845 WP_046719962.1 5068396..5069613(+) (pilC) [Pseudomonas syringae pv. syringae HS191]
MASKAVKVSVYTWEGVDKKGGKLSGEVSGHNLALVKAQLRKQGINFTKVRKKPVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIIAEGAENPNMRALVGSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPTAVLIVAFIVSGILLIKVVPQFQSVFAGFNAELPAFTLMVIALSEVVQQWWLAIVVLFFISFFMFK
RTYKRSQNFRDSVDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNQVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDAMLDKVATYYEDEVDNMVDNMTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=113035 PSYRH_RS21845 WP_046719962.1 5068396..5069613(+) (pilC) [Pseudomonas syringae pv. syringae HS191]
ATGGCCAGCAAGGCAGTTAAAGTCAGTGTTTACACATGGGAAGGTGTAGACAAGAAAGGCGGCAAACTCAGTGGCGAAGT
GAGCGGGCACAATCTTGCGCTCGTAAAGGCTCAACTGCGCAAGCAAGGTATCAACTTCACCAAGGTACGTAAAAAGCCCG
TTTCGATATTTGGCAAAGGCAAGAAGATCAAGCCGCTGGACATCGCATTCTTTTCTCGGCAGATGGCAACCATGATGAAG
GCAGGTGTGCCGCTTCTGCAGTCATTTGACATCATTGCCGAGGGTGCAGAAAACCCGAACATGCGTGCACTGGTTGGCTC
ACTGAAGCAGGAGGTGTCGGCAGGGAACAGCTTTGCGACGGCTCTGCGGCAGAAACCAGAATACTTTGATGATTTGTTCT
GCAACCTGGTCGATGCCGGCGAACAAGCGGGAGCGTTAGAAAGCCTGCTGGATCGCGTGGCAAGCTACAAGGAAAAAACA
GAAAAGCTTAAGGCCAAAATCAAGAAGGCCATGACTTATCCTACCGCCGTATTAATTGTCGCGTTTATCGTGTCAGGTAT
TTTGCTGATCAAGGTGGTGCCGCAGTTCCAGTCTGTTTTTGCAGGGTTCAACGCGGAATTACCAGCGTTCACATTGATGG
TCATCGCGCTTTCGGAGGTCGTTCAGCAGTGGTGGCTGGCGATAGTCGTATTGTTTTTTATCAGCTTTTTCATGTTTAAG
AGGACATACAAGCGGTCTCAAAATTTTCGTGACAGCGTCGACCGCTTTCTTCTGAAAGTACCCATTATCGGACCGCTGAT
TTTCAAGTCATCCGTCGCACGTTATGCACGCACGCTTGCGACAACCTTTGCCGCAGGGGTGCCACTGGTTGAGGCACTGG
ATTCGGTTGCCGGAGCCACCGGTAACGTCGTATTCAGGAACGCGGTCAATCAAGTCAAACAGGATGTCTCTACTGGCATG
CAGCTGAATTTCTCGATGCGATCAACAGGTGTATTTCCAAGTCTGGCGATACAAATGACAGCAATCGGAGAAGAGTCAGG
GGCGCTTGACGCCATGCTCGATAAAGTCGCGACTTACTATGAAGACGAGGTCGATAATATGGTCGACAATATGACCAGTC
TCATGGAACCGATGATCATGGCGGTGCTGGGTGTTATCGTCGGCGGCTTAGTGATTGCCATGTATCTCCCCATCTTCAAA
CTTGGAAGCGTTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.333

100

0.733
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Acinetobacter baumannii D1279779

58.168

99.753

0.58
  pilC Legionella pneumophila strain ERS1305867

51.768

97.778

0.506
  pilG Neisseria gonorrhoeae MS11

42.432

99.506

0.422
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.769

100

0.42
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Thermus thermophilus HB27

38.06

99.259

0.378

Multiple sequence alignment