Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSYRH_RS21840 Genome accession   NZ_CP006256
Coordinates   5066699..5068393 (+) Length   564 a.a.
NCBI ID   WP_046719961.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae HS191     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5061699..5073393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYRH_RS21820 (PsyrH_22295) - 5062619..5063164 (+) 546 WP_046719958.1 DUF1523 family protein -
  PSYRH_RS21825 (PsyrH_22300) - 5063214..5064500 (-) 1287 WP_046719959.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  PSYRH_RS21830 (PsyrH_22305) - 5064611..5065915 (-) 1305 WP_046720349.1 O-antigen ligase family protein -
  PSYRH_RS27325 - 5066078..5066473 (-) 396 WP_046719960.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PSYRH_RS21840 (PsyrH_22310) pilB 5066699..5068393 (+) 1695 WP_046719961.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSYRH_RS21845 (PsyrH_22315) pilC 5068396..5069613 (+) 1218 WP_046719962.1 type II secretion system F family protein Machinery gene
  PSYRH_RS21850 (PsyrH_22320) pilD 5069615..5070508 (+) 894 WP_046719963.1 A24 family peptidase Machinery gene
  PSYRH_RS21855 (PsyrH_22325) coaE 5070505..5071128 (+) 624 WP_046719964.1 dephospho-CoA kinase -
  PSYRH_RS21860 (PsyrH_22330) yacG 5071125..5071334 (+) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PSYRH_RS21865 (PsyrH_22335) - 5071460..5071921 (-) 462 WP_046720350.1 hypothetical protein -
  PSYRH_RS21870 (PsyrH_22340) - 5072049..5072678 (+) 630 WP_003430735.1 DUF1780 domain-containing protein -
  PSYRH_RS21875 (PsyrH_22345) - 5072675..5073208 (+) 534 WP_003317783.1 MOSC domain-containing protein -
  PSYRH_RS29100 - 5073093..5073329 (+) 237 WP_223886507.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62477.75 Da        Isoelectric Point: 6.0143

>NTDB_id=113034 PSYRH_RS21840 WP_046719961.1 5066699..5068393(+) (pilB) [Pseudomonas syringae pv. syringae HS191]
MSDAVLTGLAKQLVLAELLTETAAQQAYQQARRDKVSLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLIRQHHALPLWRRGNKLFVGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDTESGLGELADIDLGLD
IEPASDKETSLATQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVAKPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKNKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSQCKKELDIPRETLIQEGFPEEK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=113034 PSYRH_RS21840 WP_046719961.1 5066699..5068393(+) (pilB) [Pseudomonas syringae pv. syringae HS191]
ATGTCTGATGCTGTCCTCACCGGTTTGGCCAAACAATTGGTCTTGGCCGAGCTACTCACCGAAACTGCTGCGCAGCAAGC
TTATCAGCAGGCGCGCCGCGACAAGGTTTCACTGGTGAGCTATCTGGTCCAGAACAAACTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCAGACCAGTTTGGCGTACCTTTCATGGACCTTGCCAGTCTGGACAAGGAAAGCCAACCCAAAGGGTTG
GTCAGTGAAAAGCTGATTCGTCAGCATCATGCACTTCCGTTGTGGCGGCGTGGTAACAAGCTGTTTGTAGGCATCTCGGA
CCCGACCAACCATCAGGCGGTCACAGACATACAGTTCAGTACCGGGCTAAACACTGAAGCGATTCTGGTTGAAGATGACA
AGCTGACCATTGCCATAGACCGTTTTTTTGACACTGAAAGTGGCCTTGGGGAACTGGCGGATATCGACCTTGGCCTGGAC
ATTGAGCCTGCAAGTGACAAGGAAACCTCCCTGGCCACTCAGAGTGATGCCGATGATGCTCCAGTGGTGCGCTTCGTCAA
CAAAATGCTGATGGATGCAATACGCCTAGGGTCATCCGACCTGCACTTTGAACCTTACGAAAAGATCTTTCGCGTGCGCC
TGCGCACCGACGGTATTTTGCATGAAGTGGCCAAACCACCGATTCATCTGGCCAACCGAATTGCTGCGCGACTGAAAGTT
ATGGCCAGCCTCGATATCTCGGAACGACGAAAACCGCAGGACGGCAGGGTCAAGCTGCGAGTGTCAAAAAACAAAGCGAT
CGACTTCCGCATGAACACACTGCCAACACTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGATCCGACCAGCGCACAGA
TGGGCATTGACGCATTGGGCTATGAACCGGACCAAAAAGCTTTGTATATGGAAGCCTTGAAACAACCCCAAGGCATGATT
CTTGTGACCGGCCCCACCGGCTCGGGTAAAACCGTTTCGCTATATACCGGCCTGAACATTCTCAACACCGTGGACATCAA
CATTTCCACTGCTGAAGACCCAGTAGAAATCAATCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGTCAGGGACTGG
ACTTTTCTCAAGCCTTGCGCGCATTTCTTCGTCAAGATCCCGACGTGATCATGGTCGGCGAGATACGAGACTTGGAAACC
GCCGAAATTGCCATCAAAGCCTCACAGACCGGTCACATGGTGTTATCTACCCTGCACACTAACAGTGCCGCAGAAACTCT
GACGCGCTTGCATCATATGGGTGTTGCAGCGTTCAACATCGCTACAGCAATCAATCTCATCATTGCCCAGCGGCTGGCCC
GCAAGCTCTGTAGCCAATGCAAGAAAGAACTCGATATACCTCGTGAAACGCTCATCCAGGAGGGCTTTCCGGAAGAAAAG
ATAGGTACGTTCAAAATCTACGGTCCCGTCGGCTGCGAGCATTGCAATGGTGGCTACAGGGGCCGGGTCGGCATTTACGA
AGTAGTCAAGAAAACACCAGAACTGGAACGCATCATCATGGAAGAAGGCAATTCGCTGGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAATGATTTGAGGACCTCCGGGCTGTCGAAGGCCATGCAGGGCATTACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.22

100

0.592
  pilB Acinetobacter baylyi ADP1

57.651

99.645

0.574
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.56
  pilB Vibrio cholerae strain A1552

50.802

99.468

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.542

98.227

0.496
  pilB Vibrio campbellii strain DS40M4

49.291

100

0.493
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

39.046

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.469

93.44

0.369

Multiple sequence alignment