Detailed information NTG1128274

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: E5P1_RS23475
• Genome accession: NZ_LR594689
• Coordinates: 4850907..4852130 (-)
• Length: 407 a.a.
• NCBI ID: WP_068679963.1
• Uniprot ID: A0A2J7V927
• Organism: Variovorax sp. WDL1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4845907..4857130

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E5P1_RS23450 (G3W93_RS23440)	zapD	4845945..4846700 (-)	756	WP_068679953.1	cell division protein ZapD	-
E5P1_RS23455 (G3W93_RS23445)	-	4846738..4847754 (-)	1017	WP_068679955.1	HAD family hydrolase	-
E5P1_RS23460 (G3W93_RS23450)	-	4847765..4849258 (-)	1494	WP_068679957.1	MBOAT family protein	-
E5P1_RS23465 (G3W93_RS23455)	coaE	4849346..4849984 (-)	639	WP_068679959.1	dephospho-CoA kinase	-
E5P1_RS23470 (G3W93_RS23460)	-	4849942..4850907 (-)	966	WP_068679961.1	A24 family peptidase	-
E5P1_RS23475 (G3W93_RS23465)	pilC	4850907..4852130 (-)	1224	WP_068679963.1	type II secretion system F family protein	Machinery gene
E5P1_RS23480 (G3W93_RS23470)	pilB	4852146..4853879 (-)	1734	WP_068679965.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 407 a.a.
• Molecular weight: 44563.35 Da
• Isoelectric Point: 9.6463

>NTDB_id=1128274 E5P1_RS23475 WP_068679963.1 4850907..4852130(-) (pilC) [Variovorax sp. WDL1]
MATAAASRTTLKEYVFEWEGKDRNGKVVRGEIRAAGENQVQAALRRQGVLASKIKKRRMRSGKAIKPKDIAIFTRQLATM
MKAGVPLLQAFDIVGRGNANPSVAKLLNDIRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEELLDRLATYME
KTEAIKSKIKSALMYPTSVVVVAFVVVAIIMIFVIPAFKQVFTSFGADLPAPTLFVMAMSEFFVAYWWLIFGVIGGGLYF
FFQAWKRNERVQRFMDRLLLRVPIFGALIEKSCVARWTRTLATMFAAGVPLVEALDSVGGASGNTVYGDATVKIQQEVST
GTSLTMAMTNANLFPSMVLQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGVIIGGIVVSMYLPI
FKLGQVV

Nucleotide

• Length: 1224 bp

>NTDB_id=1128274 E5P1_RS23475 WP_068679963.1 4850907..4852130(-) (pilC) [Variovorax sp. WDL1]
ATGGCAACAGCAGCAGCATCCCGAACGACGCTCAAGGAATACGTCTTCGAGTGGGAAGGCAAGGACCGCAACGGCAAGGT
GGTGCGCGGCGAGATCCGGGCCGCCGGCGAGAACCAGGTCCAGGCCGCGCTGCGCCGCCAGGGCGTGCTGGCCTCGAAGA
TCAAGAAGCGGCGCATGCGCTCGGGCAAGGCCATCAAGCCCAAGGACATTGCCATCTTCACGCGCCAGCTGGCGACCATG
ATGAAGGCCGGCGTCCCGCTGCTGCAGGCCTTCGACATCGTGGGCCGCGGCAATGCCAACCCGAGCGTGGCCAAGCTGCT
CAACGACATCCGCAGCGATGTCGAGACCGGCACTTCGCTGTCCGCCGCCTTCCGCAAGTTCCCGAAGTACTTCGACAACC
TCTACTGCAACCTGGTCGAGGCCGGCGAGGCCGCCGGTATCCTGGAAGAACTGCTCGACCGCCTGGCCACCTACATGGAG
AAGACCGAGGCCATCAAGTCGAAGATCAAGTCGGCGCTGATGTACCCCACGTCCGTGGTCGTGGTGGCCTTCGTGGTGGT
GGCGATCATCATGATCTTCGTCATCCCGGCCTTCAAGCAGGTGTTCACCTCCTTCGGCGCCGACCTGCCGGCGCCGACGC
TCTTCGTGATGGCCATGAGCGAGTTCTTCGTTGCCTACTGGTGGCTGATCTTCGGCGTGATCGGCGGCGGGCTGTACTTC
TTCTTCCAGGCCTGGAAGCGCAACGAGCGCGTCCAGCGCTTCATGGACCGGCTCCTGCTGCGCGTGCCGATCTTCGGCGC
CCTGATCGAGAAATCCTGCGTCGCCCGCTGGACCCGCACCCTGGCCACCATGTTCGCGGCGGGCGTGCCCCTCGTGGAGG
CCCTCGATTCGGTGGGCGGCGCCTCGGGCAACACGGTGTACGGCGACGCCACGGTCAAGATCCAGCAGGAAGTCTCGACC
GGCACCAGCTTGACGATGGCCATGACCAACGCCAACCTGTTCCCCTCGATGGTGCTGCAGATGACTGCCATCGGCGAAGA
GTCCGGCTCCATCGACCACATGCTGGGCAAGGCGGCCGATTTCTACGAGTCGGAGGTCGACGACATGGTCGCGGGCCTGT
CGAGCCTGATGGAGCCGATCATCATCGTGTTCCTCGGGGTGATCATCGGCGGCATCGTGGTCTCGATGTACCTGCCCATC
TTCAAGCTCGGCCAGGTCGTCTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (277-399)

• Type II secretion system (T2SS), protein F (T2SSF) (73-196)

3D structure

• AlphaFold DB: A0A2J7V927

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Pseudomonas stutzeri DSM 10701	53.465	99.263	0.531
pilG	Neisseria gonorrhoeae MS11	52.645	97.543	0.514
pilC	Acinetobacter baylyi ADP1	51.106	100	0.511
pilG	Neisseria meningitidis 44/76-A	52.393	97.543	0.511
pilC	Legionella pneumophila strain ERS1305867	50.505	97.297	0.491
pilC	Acinetobacter baumannii D1279779	49.37	97.543	0.482
pilC	Thermus thermophilus HB27	39.905	100	0.413
pilC	Vibrio cholerae strain A1552	41.75	98.28	0.41
pilC	Vibrio campbellii strain DS40M4	40.049	100	0.4