Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E5P1_RS23480 Genome accession   NZ_LR594689
Coordinates   4852146..4853879 (-) Length   577 a.a.
NCBI ID   WP_068679965.1    Uniprot ID   -
Organism   Variovorax sp. WDL1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4847146..4858879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P1_RS23460 (G3W93_RS23450) - 4847765..4849258 (-) 1494 WP_068679957.1 MBOAT family protein -
  E5P1_RS23465 (G3W93_RS23455) coaE 4849346..4849984 (-) 639 WP_068679959.1 dephospho-CoA kinase -
  E5P1_RS23470 (G3W93_RS23460) - 4849942..4850907 (-) 966 WP_068679961.1 A24 family peptidase -
  E5P1_RS23475 (G3W93_RS23465) pilC 4850907..4852130 (-) 1224 WP_068679963.1 type II secretion system F family protein Machinery gene
  E5P1_RS23480 (G3W93_RS23470) pilB 4852146..4853879 (-) 1734 WP_068679965.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62481.71 Da        Isoelectric Point: 5.6368

>NTDB_id=1128275 E5P1_RS23480 WP_068679965.1 4852146..4853879(-) (pilB) [Variovorax sp. WDL1]
MAAADPLAKESASIALPGLARALISAGKLPAKTAEEIYQKSLSGRSSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
VDSQRLPKDLLDSKLCQAYRVVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVVAEYDKLSRMIEAAAVSAAETI
DSIVGAEFEFDDSSIAETADANEAAIAEVEDAPVVRFLHKMLLDAFSMRASDIHFEPYEHQYRVRFRIDGELREVATPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARLGIDALGYDADEKER
LMQAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNDKAGLTFATALRAFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCKAPTEVPR
EALLDAGFKDRDIDGTWKPYRPVGCPSCNGGYKGRVGIYQVMPISEEIQKIILRDGSALEIARQSEAEGVRSLRSSGLRK
VMQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1128275 E5P1_RS23480 WP_068679965.1 4852146..4853879(-) (pilB) [Variovorax sp. WDL1]
ATGGCCGCTGCTGACCCTCTCGCCAAAGAAAGCGCATCGATCGCCCTGCCCGGGCTGGCGCGCGCCTTGATCTCCGCCGG
CAAGTTGCCGGCGAAGACCGCGGAGGAGATCTATCAGAAGTCCCTCAGCGGGCGCAGCAGCTTCATCGCCGAGCTGACCG
GCACGGGCGCGGTTTCCGCGGCGGATCTCGCCCACACCCTGTCGAGTGCCTTCGGCGCCCCCCTGCTCGACCTCGACGCG
GTCGATTCCCAGCGCCTGCCCAAGGACCTGCTCGACTCCAAGCTGTGCCAAGCCTACCGGGTGGTGGTGCTGAGCAAGCG
CAACAATCGTCTCATTGTCGCAACAGCCGACCCCTCGGACCAGCAGGCGGCGGAAAAAATAAAGTTCGCGTCGCAGATGG
GCGTGGATTGGGTCGTTGCCGAGTACGACAAGCTCTCGCGCATGATCGAGGCAGCGGCGGTGAGCGCCGCCGAAACCATC
GACAGCATCGTCGGCGCCGAGTTCGAGTTCGACGACTCCTCGATCGCCGAGACCGCCGACGCGAACGAAGCGGCGATTGC
CGAAGTCGAGGACGCGCCGGTCGTCCGCTTCCTGCACAAGATGCTGCTCGACGCCTTCAGCATGCGCGCGTCGGACATCC
ACTTCGAGCCCTACGAGCACCAGTACCGCGTGCGCTTCCGGATCGACGGCGAGCTGCGCGAGGTCGCCACGCCGCCCACC
GTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTGATTTCGCGGCTGGACATCTCCGAGAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGCCCCGACCGGGTGATCGACTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGGTGCGTATCCTCGACCCGAGCAGTGCGCGCCTGGGGATCGATGCGCTTGGCTACGACGCCGACGAGAAAGAGCGG
CTGATGCAGGCCATCGGCCGTCCCTACGGCATGGTTCTTGTCACCGGGCCGACGGGCTCGGGCAAGACGGTTTCGCTCTA
CACCTGCCTGAACCTGCTGAACAAGCCGGGCGTCAACATCGCCACGGCGGAGGACCCGTCCGAAATCAACCTGCCCGGGG
TCAACCAGGTCAACGTCAACGACAAGGCGGGGCTGACCTTCGCAACCGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTTGAAACCGCCGACATCGCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCT
GTCGACCCTCCACACCAACGATGCGCCGACCACGCTGACGCGGATGCGCAACATGGGCATCGCGCCCTTCAACATCGCCT
CCAGCGTGATCCTGATCACCGCGCAGCGGCTCGCGCGCCGGCTGTGCACCGTGTGCAAGGCGCCGACCGAGGTGCCCAGG
GAAGCGCTGCTGGACGCCGGCTTCAAGGACCGCGACATCGACGGCACCTGGAAGCCCTACAGGCCCGTCGGCTGCCCGTC
CTGCAACGGCGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCGATCTCGGAGGAGATCCAGAAGATCATCCTGC
GCGACGGAAGCGCACTCGAGATCGCCCGGCAGTCCGAGGCCGAGGGCGTGCGCTCCCTGCGCAGCTCCGGGCTGCGCAAG
GTCATGCAGGGCCTGACTTCGCTCGAAGAAGTGCTCGCGGTCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.724

97.227

0.532
  pilB Acinetobacter baumannii D1279779

54.367

97.227

0.529
  pilF Neisseria gonorrhoeae MS11

53.464

97.574

0.522
  pilB Legionella pneumophila strain ERS1305867

49.209

98.614

0.485
  pilB Vibrio cholerae strain A1552

46.786

97.054

0.454
  pilB Vibrio campbellii strain DS40M4

45.648

97.574

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

47.655

92.374

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.018

94.454

0.397
  pilF Thermus thermophilus HB27

40

98.787

0.395

Multiple sequence alignment