Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   FGL20_RS00690 Genome accession   NZ_LR594050
Coordinates   107435..108376 (+) Length   313 a.a.
NCBI ID   WP_093959143.1    Uniprot ID   A0A4V0H0H6
Organism   Streptococcus porcinus strain NCTC10925     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 102435..113376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL20_RS00680 (NCTC10925_00139) rpoC 103228..106863 (+) 3636 WP_039984732.1 DNA-directed RNA polymerase subunit beta' -
  FGL20_RS00685 (NCTC10925_00140) - 106989..107354 (+) 366 WP_003085731.1 DUF1033 family protein -
  FGL20_RS00690 (NCTC10925_00141) comYA 107435..108376 (+) 942 WP_093959143.1 competence type IV pilus ATPase ComGA Machinery gene
  FGL20_RS00695 (NCTC10925_00142) comYB 108288..109343 (+) 1056 WP_370737019.1 competence type IV pilus assembly protein ComGB Machinery gene
  FGL20_RS00700 (NCTC10925_00143) comYC 109343..109669 (+) 327 WP_003083111.1 competence type IV pilus major pilin ComGC Machinery gene
  FGL20_RS00705 (NCTC10925_00144) comGD 109719..110057 (+) 339 WP_003085212.1 competence type IV pilus minor pilin ComGD -
  FGL20_RS00710 (NCTC10925_00145) comGE 110029..110322 (+) 294 WP_039984736.1 competence type IV pilus minor pilin ComGE -
  FGL20_RS00715 (NCTC10925_00146) comGF 110282..110743 (+) 462 WP_003082840.1 competence type IV pilus minor pilin ComGF -
  FGL20_RS00720 (NCTC10925_00147) comGG 110721..111119 (+) 399 WP_039984739.1 competence type IV pilus minor pilin ComGG -
  FGL20_RS00725 (NCTC10925_00148) comYH 111164..112120 (+) 957 WP_003084266.1 class I SAM-dependent methyltransferase Machinery gene
  FGL20_RS00730 (NCTC10925_00149) - 112176..113369 (+) 1194 WP_003085774.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35394.83 Da        Isoelectric Point: 7.8386

>NTDB_id=1128020 FGL20_RS00690 WP_093959143.1 107435..108376(+) (comYA) [Streptococcus porcinus strain NCTC10925]
MIQKTGEALVSQAVNLKAQDIYIIPYEKQYKVFMRVGDERRFIDNYDMKQMASLISHFKFVAGMNVGEKRRCQLGACDYK
GTLSESIPLRLSCVGNFRGFESLVIRLLTTVNKPLNYWFDTDQKVEKVISGRGLYLFSGPVGSGKTSLMYQLLTRIAKDK
QVITIEDPVEIQEDSLLQIQVNESIGMTYDNLIKLSLRHRPDYLIIGEIRDAETAKAVIRASLTGAVVFSTVHAKSIPGV
YKRLLELGIKSEEINHTLKFIAYQRLIAGGGLIDFAIETFETHSNQEWNQKLDHLLAEGHITQQQALSEKISD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1128020 FGL20_RS00690 WP_093959143.1 107435..108376(+) (comYA) [Streptococcus porcinus strain NCTC10925]
ATGATACAAAAAACAGGTGAAGCATTAGTTAGTCAAGCGGTAAATCTAAAGGCACAAGATATCTATATAATCCCTTATGA
AAAGCAGTATAAAGTTTTCATGAGAGTTGGAGATGAGAGGCGATTTATTGATAATTATGACATGAAACAGATGGCAAGTC
TTATTAGCCATTTTAAGTTTGTAGCAGGGATGAATGTAGGAGAAAAGAGACGCTGTCAACTTGGAGCCTGTGACTATAAG
GGAACTTTATCAGAATCCATTCCATTACGTCTTTCTTGTGTTGGGAATTTTAGAGGTTTTGAAAGTTTAGTTATCAGACT
CTTAACAACTGTTAATAAACCTCTCAATTACTGGTTTGATACTGATCAAAAAGTAGAAAAAGTTATTTCTGGAAGAGGTC
TTTATCTTTTCTCGGGTCCTGTAGGGTCGGGTAAAACGAGTCTCATGTATCAACTACTGACACGTATAGCAAAGGATAAA
CAAGTTATTACAATTGAAGATCCTGTTGAAATTCAAGAGGACAGCCTATTACAAATTCAAGTAAATGAAAGTATTGGAAT
GACTTATGATAACCTTATTAAACTTTCCCTCAGACATCGTCCAGATTATTTAATTATTGGAGAGATTCGTGATGCTGAGA
CTGCAAAAGCAGTCATTAGAGCAAGCTTAACAGGAGCTGTAGTCTTTTCTACTGTCCACGCAAAAAGTATTCCAGGAGTA
TATAAAAGATTGTTAGAACTAGGTATCAAAAGTGAAGAGATAAATCATACTCTAAAATTTATTGCGTACCAAAGGTTGAT
AGCAGGAGGAGGACTTATTGATTTTGCCATCGAGACATTCGAAACTCATTCCAATCAAGAATGGAATCAAAAACTTGATC
ACTTACTTGCAGAAGGACATATCACTCAACAACAGGCACTCTCGGAAAAAATTAGCGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V0H0H6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

63.898

100

0.639
  comYA Streptococcus mutans UA140

63.898

100

0.639
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.859

99.681

0.617
  comYA Streptococcus gordonii str. Challis substr. CH1

60.064

100

0.601
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

58.013

99.681

0.578
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

57.692

99.681

0.575
  comGA/cglA/cilD Streptococcus pneumoniae D39

57.692

99.681

0.575
  comGA/cglA/cilD Streptococcus pneumoniae R6

57.692

99.681

0.575
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

57.692

99.681

0.575
  comGA Lactococcus lactis subsp. cremoris KW2

52.244

99.681

0.521

Multiple sequence alignment