Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   FGL20_RS00725 Genome accession   NZ_LR594050
Coordinates   111164..112120 (+) Length   318 a.a.
NCBI ID   WP_003084266.1    Uniprot ID   A0A4V0GYZ8
Organism   Streptococcus porcinus strain NCTC10925     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 106164..117120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL20_RS00685 (NCTC10925_00140) - 106989..107354 (+) 366 WP_003085731.1 DUF1033 family protein -
  FGL20_RS00690 (NCTC10925_00141) comYA 107435..108376 (+) 942 WP_093959143.1 competence type IV pilus ATPase ComGA Machinery gene
  FGL20_RS00695 (NCTC10925_00142) comYB 108288..109343 (+) 1056 WP_370737019.1 competence type IV pilus assembly protein ComGB Machinery gene
  FGL20_RS00700 (NCTC10925_00143) comYC 109343..109669 (+) 327 WP_003083111.1 competence type IV pilus major pilin ComGC Machinery gene
  FGL20_RS00705 (NCTC10925_00144) comGD 109719..110057 (+) 339 WP_003085212.1 competence type IV pilus minor pilin ComGD -
  FGL20_RS00710 (NCTC10925_00145) comGE 110029..110322 (+) 294 WP_039984736.1 competence type IV pilus minor pilin ComGE -
  FGL20_RS00715 (NCTC10925_00146) comGF 110282..110743 (+) 462 WP_003082840.1 competence type IV pilus minor pilin ComGF -
  FGL20_RS00720 (NCTC10925_00147) comGG 110721..111119 (+) 399 WP_039984739.1 competence type IV pilus minor pilin ComGG -
  FGL20_RS00725 (NCTC10925_00148) comYH 111164..112120 (+) 957 WP_003084266.1 class I SAM-dependent methyltransferase Machinery gene
  FGL20_RS00730 (NCTC10925_00149) - 112176..113369 (+) 1194 WP_003085774.1 acetate kinase -
  FGL20_RS00735 (NCTC10925_00151) - 113634..113843 (+) 210 WP_093959144.1 helix-turn-helix transcriptional regulator -
  FGL20_RS00740 (NCTC10925_00152) - 113840..114292 (+) 453 WP_093959145.1 hypothetical protein -
  FGL20_RS00745 (NCTC10925_00153) - 114304..114945 (+) 642 WP_093959146.1 CPBP family intramembrane glutamic endopeptidase -
  FGL20_RS00750 (NCTC10925_00154) proC 114995..115765 (-) 771 WP_003085990.1 pyrroline-5-carboxylate reductase -
  FGL20_RS00755 (NCTC10925_00155) pepA 115783..116850 (-) 1068 WP_003083897.1 glutamyl aminopeptidase -

Sequence

Protein


Download         Length: 318 a.a.        Molecular weight: 36044.90 Da        Isoelectric Point: 4.6494

>NTDB_id=1128023 FGL20_RS00725 WP_003084266.1 111164..112120(+) (comYH) [Streptococcus porcinus strain NCTC10925]
MNFEKIEKAYELILENSQLIENEIKTHIYDALIEQNAYYLGADGASEQVLKNNGDLYALNLSKEEWRRAFQFVFIKAGQT
EKLQANHQFTPDSIAFIMLFLMEQLHSGNSLDVIEIGSGTGNLAQTLLNNSLKKIDYLGLELDDLLIDLSASIAEVMKSS
ATFLQEDAVRPQVLKESDIIISDLPVGYYPNDEIASRYQVASAEGHTYAHHLLMEQSLKYLKNNGFAIFLAPSNLLNSSQ
SDLLKKWLKDYAQLRVVVTLPDSIFGNQANAKSIIVLQKNTEKNGETFVYPLTDLQSPQTVQRFMEEFKKWKQDNVFS

Nucleotide


Download         Length: 957 bp        

>NTDB_id=1128023 FGL20_RS00725 WP_003084266.1 111164..112120(+) (comYH) [Streptococcus porcinus strain NCTC10925]
ATGAACTTTGAAAAAATTGAGAAAGCCTATGAGCTTATTTTGGAGAACAGCCAACTCATTGAAAATGAGATTAAGACGCA
TATTTATGATGCTTTAATTGAGCAGAATGCCTATTATTTAGGAGCTGATGGAGCAAGTGAACAGGTCTTGAAAAATAATG
GTGACTTATATGCTTTAAACTTGAGTAAGGAAGAGTGGCGACGCGCCTTTCAGTTTGTTTTTATAAAGGCAGGCCAGACG
GAAAAATTACAAGCTAATCACCAATTTACCCCAGACAGTATTGCTTTTATCATGCTTTTTTTGATGGAGCAGTTGCATAG
TGGAAATAGTTTGGATGTGATTGAGATTGGTAGTGGGACAGGGAATCTTGCTCAGACACTACTAAATAACAGTCTTAAGA
AAATCGACTACTTAGGTTTGGAGTTAGATGATTTATTGATTGATTTGTCAGCCAGTATAGCTGAGGTCATGAAGTCCTCT
GCAACTTTTCTTCAAGAAGATGCTGTTCGCCCGCAAGTACTAAAAGAAAGTGACATTATCATTAGTGATTTACCTGTTGG
TTATTATCCGAATGATGAGATTGCAAGTCGCTATCAGGTGGCAAGTGCTGAGGGACATACTTATGCTCATCATTTATTGA
TGGAGCAATCATTGAAGTATCTCAAAAATAATGGCTTTGCGATTTTCTTAGCACCAAGTAATCTCTTAAACAGTTCTCAA
AGTGATTTGTTGAAAAAATGGTTAAAAGACTATGCTCAGCTTAGAGTGGTCGTGACCCTGCCAGACTCTATTTTTGGCAA
TCAAGCAAATGCAAAATCCATTATCGTTCTACAAAAGAACACTGAAAAAAATGGTGAAACTTTTGTTTACCCACTGACTG
ATTTACAATCACCACAGACGGTTCAAAGGTTCATGGAAGAATTCAAAAAATGGAAACAAGATAATGTTTTTTCATAA

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(73-297)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V0GYZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA159

66.562

99.686

0.664
  comYH Streptococcus mutans UA140

66.246

99.686

0.66

Multiple sequence alignment