Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   FGL20_RS00695 Genome accession   NZ_LR594050
Coordinates   108288..109343 (+) Length   351 a.a.
NCBI ID   WP_370737019.1    Uniprot ID   -
Organism   Streptococcus porcinus strain NCTC10925     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 103288..114343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL20_RS00685 (NCTC10925_00140) - 106989..107354 (+) 366 WP_003085731.1 DUF1033 family protein -
  FGL20_RS00690 (NCTC10925_00141) comYA 107435..108376 (+) 942 WP_093959143.1 competence type IV pilus ATPase ComGA Machinery gene
  FGL20_RS00695 (NCTC10925_00142) comYB 108288..109343 (+) 1056 WP_370737019.1 competence type IV pilus assembly protein ComGB Machinery gene
  FGL20_RS00700 (NCTC10925_00143) comYC 109343..109669 (+) 327 WP_003083111.1 competence type IV pilus major pilin ComGC Machinery gene
  FGL20_RS00705 (NCTC10925_00144) comGD 109719..110057 (+) 339 WP_003085212.1 competence type IV pilus minor pilin ComGD -
  FGL20_RS00710 (NCTC10925_00145) comGE 110029..110322 (+) 294 WP_039984736.1 competence type IV pilus minor pilin ComGE -
  FGL20_RS00715 (NCTC10925_00146) comGF 110282..110743 (+) 462 WP_003082840.1 competence type IV pilus minor pilin ComGF -
  FGL20_RS00720 (NCTC10925_00147) comGG 110721..111119 (+) 399 WP_039984739.1 competence type IV pilus minor pilin ComGG -
  FGL20_RS00725 (NCTC10925_00148) comYH 111164..112120 (+) 957 WP_003084266.1 class I SAM-dependent methyltransferase Machinery gene
  FGL20_RS00730 (NCTC10925_00149) - 112176..113369 (+) 1194 WP_003085774.1 acetate kinase -
  FGL20_RS00735 (NCTC10925_00151) - 113634..113843 (+) 210 WP_093959144.1 helix-turn-helix transcriptional regulator -
  FGL20_RS00740 (NCTC10925_00152) - 113840..114292 (+) 453 WP_093959145.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 40484.33 Da        Isoelectric Point: 9.9157

>NTDB_id=1128021 FGL20_RS00695 WP_370737019.1 108288..109343(+) (comYB) [Streptococcus porcinus strain NCTC10925]
MKNGIKNLITYLQKDISLNNRHSRKKLATKKQAKLIQLLHNLSQSGFNLAEMIAFLEKTQILDRKHLSLMKTDLLNGCSL
SEMMRHLGFPDGIITQLSLAEAHGNTKKCLGKIEDYLVQTQKIRQKTIEVVTYPIILLTFLILIILGLRHYLMPQMEKQS
TLTYFLNNFPVYLLSLSIILLLTVIALVIRWRKLGKMKQMIQYSQIPLLAPFLKLYMTAFYAREWGNLISQGIELSQIVT
IMSIEKSDLIKEIGLDMRQSFLEGQSFHEKVRGYPFFSDELSLMIEYGEIKGKLGQELDIYGQLTWDAYFRKLFQATQII
QPFIFMIVALIIVMIYAAMLLPMYNTIGGTI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=1128021 FGL20_RS00695 WP_370737019.1 108288..109343(+) (comYB) [Streptococcus porcinus strain NCTC10925]
ATCAAGAATGGAATCAAAAACTTGATCACTTACTTGCAGAAGGACATATCACTCAACAACAGGCACTCTCGGAAAAAATT
AGCGACTAAAAAACAGGCCAAATTAATCCAACTCTTACATAATTTAAGTCAAAGTGGTTTTAATCTAGCAGAGATGATCG
CCTTCTTAGAAAAAACACAGATCTTAGACCGAAAACATCTTAGCTTGATGAAGACTGATTTGTTAAATGGATGTAGTTTA
TCAGAAATGATGAGGCATTTAGGATTTCCTGATGGAATTATTACCCAACTAAGTTTAGCTGAAGCTCATGGCAATACCAA
AAAATGTTTGGGTAAAATTGAAGACTATCTAGTACAGACGCAAAAGATTAGGCAAAAGACAATTGAAGTCGTGACCTATC
CCATCATTCTCCTTACTTTTTTAATTTTAATCATCCTGGGCTTGAGACATTACTTAATGCCTCAGATGGAGAAACAAAGC
ACTTTGACCTATTTTCTCAATAATTTCCCAGTTTACCTCCTAAGTTTGTCAATTATTCTTCTGCTTACGGTCATTGCTTT
GGTGATCAGATGGCGTAAACTTGGAAAGATGAAACAGATGATACAATACAGTCAGATTCCTTTGTTGGCACCCTTCCTAA
AGCTCTATATGACGGCTTTTTATGCCCGTGAATGGGGGAATTTAATTAGTCAAGGTATCGAACTGAGTCAAATAGTAACC
ATTATGAGTATTGAAAAATCTGATTTGATAAAAGAAATAGGTTTAGATATGAGACAATCATTTCTTGAAGGGCAGAGTTT
CCACGAAAAAGTAAGGGGATATCCTTTTTTTAGTGATGAGTTGAGTTTGATGATTGAATATGGTGAAATCAAAGGGAAAT
TAGGTCAGGAGCTAGATATTTATGGCCAACTGACATGGGATGCTTATTTTAGAAAACTCTTCCAAGCGACACAGATTATC
CAACCTTTCATATTTATGATTGTCGCCCTGATTATTGTAATGATTTATGCCGCTATGCTATTACCTATGTATAACACAAT
TGGAGGAACAATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(36-154)

Type II secretion system (T2SS), protein F (T2SSF)

(222-342)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

51.917

96.581

0.501
  comYB Streptococcus mutans UA159

51.622

96.581

0.499
  comGB/cglB Streptococcus mitis SK321

48.949

94.872

0.464
  comGB/cglB Streptococcus mitis NCTC 12261

48.649

94.872

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

46.547

94.872

0.442
  comGB/cglB Streptococcus pneumoniae R6

46.547

94.872

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

46.547

94.872

0.442
  comGB/cglB Streptococcus pneumoniae D39

46.547

94.872

0.442
  comYB Streptococcus gordonii str. Challis substr. CH1

45.294

96.866

0.439
  comGB Lactococcus lactis subsp. cremoris KW2

42.69

97.436

0.416

Multiple sequence alignment