Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   FGL21_RS00850 Genome accession   NZ_LR594047
Coordinates   136355..137296 (+) Length   313 a.a.
NCBI ID   WP_138127937.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae subsp. equisimilis strain NCTC11554     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 131355..142296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL21_RS00840 (NCTC11554_00172) rpoC 132065..135706 (+) 3642 WP_138127935.1 DNA-directed RNA polymerase subunit beta' -
  FGL21_RS00845 (NCTC11554_00173) - 135909..136274 (+) 366 WP_003055257.1 DUF1033 family protein -
  FGL21_RS00850 (NCTC11554_00174) comGA/cglA 136355..137296 (+) 942 WP_138127937.1 competence type IV pilus ATPase ComGA Machinery gene
  FGL21_RS00855 (NCTC11554_00175) comYB 137232..138263 (+) 1032 WP_138128262.1 competence type IV pilus assembly protein ComGB Machinery gene
  FGL21_RS00860 (NCTC11554_00176) comYC 138265..138591 (+) 327 WP_003055259.1 competence type IV pilus major pilin ComGC Machinery gene
  FGL21_RS00865 (NCTC11554_00177) comGD 138566..138994 (+) 429 WP_014611932.1 competence type IV pilus minor pilin ComGD -
  FGL21_RS00870 (NCTC11554_00178) comGE 139002..139247 (+) 246 WP_003058569.1 competence type IV pilus minor pilin ComGE -
  FGL21_RS00875 (NCTC11554_00179) comGF 139228..139668 (+) 441 WP_003061780.1 competence type IV pilus minor pilin ComGF -
  FGL21_RS00880 (NCTC11554_00180) comGG 139691..140020 (+) 330 WP_231117501.1 competence type IV pilus minor pilin ComGG -
  FGL21_RS00885 (NCTC11554_00181) comYH 140083..141036 (+) 954 WP_003060701.1 class I SAM-dependent methyltransferase Machinery gene
  FGL21_RS00890 (NCTC11554_00182) - 141095..142291 (+) 1197 WP_003058485.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35179.32 Da        Isoelectric Point: 7.5611

>NTDB_id=1127832 FGL21_RS00850 WP_138127937.1 136355..137296(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC11554]
MVQALARSILKEAEQIQAQDIYILPKENQYELLIRVGDERRLVDVYRGDRMAHLISHFKFVAGMTVGEKRRCQVGSCDYD
IGGDTLLSLRLSSVGNYRGQESLVIRLLHHQQRSLHYWFDGLKTVARQIGGRGLYLFAGPVGSGKTTLMHQLIADYYQGA
QVISIEDPVEIKNHQALQLQVNDSIGMTYDNLIKLSLRHRPDILIIGEIRDSQTARAVIRASLTGVLVFSTVHAKSISGV
YARLLELGVTRAELDNCLALVAYQRLLNGGALIDSTQTEFEHYSPNQWNQKIDELLAAGHLSPEQARFEKIIQ

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1127832 FGL21_RS00850 WP_138127937.1 136355..137296(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC11554]
ATGGTACAAGCATTAGCAAGATCTATTTTGAAAGAAGCTGAGCAGATTCAAGCTCAAGATATCTATATTTTGCCAAAGGA
AAATCAGTATGAGCTATTGATAAGGGTAGGAGATGAAAGGAGATTGGTGGATGTTTATCGGGGCGATCGGATGGCTCATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGACCGTTGGTGAGAAACGACGCTGTCAAGTCGGTTCTTGTGATTATGAT
ATCGGTGGAGACACACTTTTGTCTCTACGCCTGTCTAGTGTTGGAAATTACCGTGGACAAGAGAGCCTAGTGATTCGGTT
ATTACATCATCAACAAAGAAGTTTACATTACTGGTTTGACGGACTGAAAACAGTTGCACGTCAGATTGGAGGCCGAGGAC
TCTATCTTTTTGCAGGTCCAGTCGGATCAGGCAAAACGACTTTGATGCACCAATTGATTGCTGATTATTATCAAGGAGCA
CAGGTCATTAGCATAGAAGATCCGGTGGAAATTAAAAATCATCAAGCGCTCCAATTACAAGTTAATGATAGTATTGGTAT
GACTTACGACAACTTGATCAAACTATCTTTACGGCATCGCCCTGATATTTTAATCATTGGTGAAATTCGAGACAGCCAGA
CAGCTAGAGCGGTTATTAGAGCAAGTTTAACAGGTGTCTTAGTATTTTCAACAGTTCATGCCAAAAGCATTTCTGGTGTT
TATGCCAGATTACTAGAACTAGGAGTGACAAGAGCAGAATTAGACAATTGTTTGGCTTTGGTTGCTTACCAACGATTGCT
TAATGGAGGAGCATTGATTGACTCAACTCAAACAGAATTTGAACACTACTCACCAAACCAATGGAATCAGAAAATTGATG
AGCTTCTTGCAGCGGGACATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.935

99.042

0.613
  comYA Streptococcus mutans UA140

61.093

99.361

0.607
  comYA Streptococcus mutans UA159

61.093

99.361

0.607
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.361

0.604
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.486

99.361

0.591
  comGA Lactococcus lactis subsp. cremoris KW2

52.412

99.361

0.521
  comGA Latilactobacillus sakei subsp. sakei 23K

38.908

93.61

0.364

Multiple sequence alignment