Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   FGL21_RS00855 Genome accession   NZ_LR594047
Coordinates   137232..138263 (+) Length   343 a.a.
NCBI ID   WP_138128262.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae subsp. equisimilis strain NCTC11554     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 132232..143263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL21_RS00845 (NCTC11554_00173) - 135909..136274 (+) 366 WP_003055257.1 DUF1033 family protein -
  FGL21_RS00850 (NCTC11554_00174) comGA/cglA 136355..137296 (+) 942 WP_138127937.1 competence type IV pilus ATPase ComGA Machinery gene
  FGL21_RS00855 (NCTC11554_00175) comYB 137232..138263 (+) 1032 WP_138128262.1 competence type IV pilus assembly protein ComGB Machinery gene
  FGL21_RS00860 (NCTC11554_00176) comYC 138265..138591 (+) 327 WP_003055259.1 competence type IV pilus major pilin ComGC Machinery gene
  FGL21_RS00865 (NCTC11554_00177) comGD 138566..138994 (+) 429 WP_014611932.1 competence type IV pilus minor pilin ComGD -
  FGL21_RS00870 (NCTC11554_00178) comGE 139002..139247 (+) 246 WP_003058569.1 competence type IV pilus minor pilin ComGE -
  FGL21_RS00875 (NCTC11554_00179) comGF 139228..139668 (+) 441 WP_003061780.1 competence type IV pilus minor pilin ComGF -
  FGL21_RS00880 (NCTC11554_00180) comGG 139691..140020 (+) 330 WP_231117501.1 competence type IV pilus minor pilin ComGG -
  FGL21_RS00885 (NCTC11554_00181) comYH 140083..141036 (+) 954 WP_003060701.1 class I SAM-dependent methyltransferase Machinery gene
  FGL21_RS00890 (NCTC11554_00182) - 141095..142291 (+) 1197 WP_003058485.1 acetate kinase -
  FGL21_RS00895 (NCTC11554_00183) - 142597..142803 (+) 207 WP_003058508.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 38939.31 Da        Isoelectric Point: 9.2055

>NTDB_id=1127833 FGL21_RS00855 WP_138128262.1 137232..138263(+) (comYB) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC11554]
MSFLQRDISVRSKQGLKKLSSKEQYKLIQLLANLLLTGFNLAEIIAFLTKSHLIPEPYVLRMEEALLKGRGLADMLAGIG
FSDAILTQISLADGHGNIETTLVNIQGYLNQMAKLKRKTIEVVTYPIILLVFLFVMILGLRHYVMPQLEAQNQLTIFLTH
FPAIFFGIILFIGVLLGGIWLHWRSQSRLKLFTHFSAYPVLGKLLQQYLTAYYAREWGTLIGQGLELPVILDIMAMEKSL
FMQELAEDIRVSLLAGQAFHDKVATYPFFKKELSLMITYGEIKSKLGSELEIYAQESWTQFFSQLHQATQFIQPIIFLVI
ALTIVMIYAAILLPIYQNMGGVL

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=1127833 FGL21_RS00855 WP_138128262.1 137232..138263(+) (comYB) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC11554]
ATGAGCTTCTTGCAGCGGGACATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAAGGAACAATACAAACT
AATTCAGCTATTAGCTAACCTTTTGTTAACAGGATTTAACTTGGCTGAGATAATAGCTTTCTTAACAAAAAGTCATTTGA
TTCCCGAGCCCTATGTTCTCCGCATGGAAGAAGCACTCTTAAAAGGTCGAGGGCTGGCAGATATGTTAGCTGGGATAGGT
TTTTCGGATGCTATTTTAACTCAAATTAGTTTAGCAGATGGTCACGGAAACATTGAGACGACGCTTGTTAATATTCAGGG
CTATCTCAACCAAATGGCTAAACTTAAACGAAAAACCATTGAAGTCGTCACCTATCCGATTATTCTCCTGGTATTTCTTT
TTGTGATGATATTAGGGTTAAGGCATTATGTTATGCCTCAATTAGAAGCTCAAAATCAGCTTACTATTTTTCTGACTCAT
TTTCCAGCTATTTTCTTTGGTATTATCCTTTTTATAGGTGTGCTGTTAGGGGGAATTTGGCTGCATTGGCGATCGCAAAG
TCGCCTCAAGCTTTTTACTCACTTCAGTGCTTATCCTGTTTTAGGGAAACTCTTACAACAGTATCTGACGGCTTATTATG
CTAGAGAATGGGGAACATTGATTGGACAGGGACTTGAATTACCAGTCATTCTGGATATTATGGCCATGGAGAAATCTTTA
TTCATGCAAGAATTGGCTGAAGATATTCGAGTTAGTTTATTGGCAGGACAAGCTTTTCATGATAAGGTAGCTACCTATCC
TTTCTTTAAAAAAGAGTTAAGTTTGATGATTACTTATGGAGAGATTAAATCCAAGTTAGGAAGTGAACTAGAGATTTATG
CTCAAGAGAGTTGGACTCAGTTTTTCAGCCAACTTCATCAAGCAACTCAGTTTATTCAACCGATTATCTTTTTAGTGATT
GCTCTGACGATTGTCATGATTTATGCAGCAATTTTATTACCAATTTATCAAAATATGGGAGGCGTTTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-146)

Type II secretion system (T2SS), protein F (T2SSF)

(214-334)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.097

98.834

0.525
  comYB Streptococcus mutans UA159

53.097

98.834

0.525
  comYB Streptococcus gordonii str. Challis substr. CH1

48.968

98.834

0.484
  comGB/cglB Streptococcus mitis NCTC 12261

45.97

97.668

0.449
  comGB/cglB Streptococcus mitis SK321

45.345

97.085

0.44
  comGB/cglB Streptococcus pneumoniae Rx1

43.844

97.085

0.426
  comGB/cglB Streptococcus pneumoniae D39

43.844

97.085

0.426
  comGB/cglB Streptococcus pneumoniae R6

43.844

97.085

0.426
  comGB/cglB Streptococcus pneumoniae TIGR4

43.844

97.085

0.426
  comGB Lactococcus lactis subsp. cremoris KW2

42.773

98.834

0.423

Multiple sequence alignment