Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   FGL00_RS00725 Genome accession   NZ_LR594034
Coordinates   119669..120610 (+) Length   313 a.a.
NCBI ID   WP_138098129.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae subsp. equisimilis strain NCTC8546     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114669..125610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL00_RS00715 (NCTC8546_00147) rpoC 115379..119020 (+) 3642 WP_037589802.1 DNA-directed RNA polymerase subunit beta' -
  FGL00_RS00720 (NCTC8546_00148) - 119223..119588 (+) 366 WP_003055257.1 DUF1033 family protein -
  FGL00_RS00725 (NCTC8546_00149) comGA/cglA 119669..120610 (+) 942 WP_138098129.1 competence type IV pilus ATPase ComGA Machinery gene
  FGL00_RS00730 (NCTC8546_00150) comYB 120546..121577 (+) 1032 WP_138098784.1 competence type IV pilus assembly protein ComGB Machinery gene
  FGL00_RS00735 (NCTC8546_00151) comYC 121579..121905 (+) 327 WP_003055259.1 competence type IV pilus major pilin ComGC Machinery gene
  FGL00_RS00740 (NCTC8546_00152) comGD 121880..122308 (+) 429 WP_014611932.1 competence type IV pilus minor pilin ComGD -
  FGL00_RS00745 (NCTC8546_00153) comGE 122316..122561 (+) 246 WP_003058569.1 competence type IV pilus minor pilin ComGE -
  FGL00_RS00750 (NCTC8546_00154) comGF 122542..122982 (+) 441 WP_138098131.1 competence type IV pilus minor pilin ComGF -
  FGL00_RS00755 (NCTC8546_00155) comGG 123005..123334 (+) 330 WP_003058539.1 competence type IV pilus minor pilin ComGG -
  FGL00_RS00760 (NCTC8546_00156) comYH 123397..124350 (+) 954 WP_003060701.1 class I SAM-dependent methyltransferase Machinery gene
  FGL00_RS00765 (NCTC8546_00157) - 124409..125605 (+) 1197 WP_138098133.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35112.24 Da        Isoelectric Point: 7.2699

>NTDB_id=1127216 FGL00_RS00725 WP_138098129.1 119669..120610(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC8546]
MVQALARSILKEAEQIQAQDIYILPKENQYELLIRVGDERRLVDVYRGDRMAHLISHFKFVAGMTVGEKRRCQVGSCDYD
IGGDTLLSLRLSSVGNYRGQESLVIRLLHHQQRSLHYWFDGLKTVACQIGGRGLYLFAGPVGSGKTTLMHQLIADYYQGA
QVISIEDPVEIKNHQALQLQVNDSIGMTYDNLIKLSLRHRPDILIIGEIRDSQTARAVIRASLTGVLVFSTVHAKSISGV
YARLLELGVTRAELDNCLALVAYQRLLNGGALIDSTQTEFEHYSPNQWNQKIDDLLAAGHLSPEQARFEKIIQ

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1127216 FGL00_RS00725 WP_138098129.1 119669..120610(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC8546]
ATGGTACAAGCATTAGCAAGATCTATTTTGAAAGAAGCTGAGCAGATTCAAGCTCAAGATATCTATATTTTGCCAAAGGA
AAATCAGTATGAGCTATTGATAAGGGTAGGAGATGAAAGGAGATTGGTGGATGTTTATCGGGGCGATCGGATGGCTCATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGACCGTTGGTGAGAAACGACGCTGTCAAGTCGGTTCTTGTGATTATGAT
ATCGGTGGAGACACACTTTTGTCTCTACGCCTGTCTAGTGTTGGAAATTACCGTGGACAAGAGAGCCTAGTGATTCGGTT
ATTACATCATCAACAAAGAAGTTTACATTACTGGTTTGACGGACTGAAAACAGTTGCATGTCAGATTGGAGGCCGAGGAC
TCTATCTTTTTGCAGGTCCAGTCGGATCAGGCAAAACGACTTTGATGCACCAATTGATTGCTGATTATTATCAAGGAGCA
CAGGTCATTAGCATAGAAGATCCGGTGGAAATTAAAAATCATCAAGCGCTCCAATTACAAGTTAATGATAGTATTGGCAT
GACTTACGACAACTTGATCAAACTATCTTTACGGCATCGCCCTGATATTTTAATCATTGGTGAAATTCGAGACAGCCAGA
CAGCTAGAGCGGTTATTAGAGCAAGTTTAACAGGTGTCTTAGTATTTTCAACAGTTCATGCCAAAAGCATTTCTGGTGTT
TATGCCAGATTACTAGAACTAGGAGTGACAAGAGCAGAATTAGACAATTGTTTGGCTTTGGTTGCTTACCAACGATTGCT
TAATGGAGGAGCATTGATTGACTCAACTCAAACAGAATTTGAACACTACTCACCAAACCAATGGAATCAGAAAATTGATG
ACCTTCTTGCAGCGGGACATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.935

99.042

0.613
  comYA Streptococcus mutans UA140

61.415

99.361

0.61
  comYA Streptococcus mutans UA159

61.415

99.361

0.61
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.361

0.604
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.486

99.361

0.591
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.486

99.361

0.591
  comGA Lactococcus lactis subsp. cremoris KW2

52.564

99.681

0.524

Multiple sequence alignment