Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   FGL00_RS00730 Genome accession   NZ_LR594034
Coordinates   120546..121577 (+) Length   343 a.a.
NCBI ID   WP_138098784.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae subsp. equisimilis strain NCTC8546     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 115546..126577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL00_RS00720 (NCTC8546_00148) - 119223..119588 (+) 366 WP_003055257.1 DUF1033 family protein -
  FGL00_RS00725 (NCTC8546_00149) comGA/cglA 119669..120610 (+) 942 WP_138098129.1 competence type IV pilus ATPase ComGA Machinery gene
  FGL00_RS00730 (NCTC8546_00150) comYB 120546..121577 (+) 1032 WP_138098784.1 competence type IV pilus assembly protein ComGB Machinery gene
  FGL00_RS00735 (NCTC8546_00151) comYC 121579..121905 (+) 327 WP_003055259.1 competence type IV pilus major pilin ComGC Machinery gene
  FGL00_RS00740 (NCTC8546_00152) comGD 121880..122308 (+) 429 WP_014611932.1 competence type IV pilus minor pilin ComGD -
  FGL00_RS00745 (NCTC8546_00153) comGE 122316..122561 (+) 246 WP_003058569.1 competence type IV pilus minor pilin ComGE -
  FGL00_RS00750 (NCTC8546_00154) comGF 122542..122982 (+) 441 WP_138098131.1 competence type IV pilus minor pilin ComGF -
  FGL00_RS00755 (NCTC8546_00155) comGG 123005..123334 (+) 330 WP_003058539.1 competence type IV pilus minor pilin ComGG -
  FGL00_RS00760 (NCTC8546_00156) comYH 123397..124350 (+) 954 WP_003060701.1 class I SAM-dependent methyltransferase Machinery gene
  FGL00_RS00765 (NCTC8546_00157) - 124409..125605 (+) 1197 WP_138098133.1 acetate kinase -
  FGL00_RS00770 (NCTC8546_00158) - 125911..126117 (+) 207 WP_003058508.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 38967.37 Da        Isoelectric Point: 9.2055

>NTDB_id=1127217 FGL00_RS00730 WP_138098784.1 120546..121577(+) (comYB) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC8546]
MTFLQRDISVRSKQGLKKLSSKEQYKLIQLLANLLLTGFNLAEIIAFLTKSHLIPEPYVLRMEEALLKGRGLADMLAGIG
FSDAILTQISLADGHGNIETTLVNIQGYLNQMAKLKRKTIEVVTYPIILLVFLFVMILGLRHYVMPQLEAQNQLTIFLTH
FPAIFFGIILFIGVLLGGIWLHWRSQSRLKLFTHFSAYPVLGKLLQQYLTAYYAREWGTLIGQGLELPVILDIMAMEKSL
FMQELAEDIRVSLLAGQAFHDKVATYPFFKKELSLMITYGEIKTKLGSELEIYAQESWTQFFSQLHQATQFIQPIIFLVI
ALTIVMIYAAILLPIYQNMGGVL

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=1127217 FGL00_RS00730 WP_138098784.1 120546..121577(+) (comYB) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC8546]
ATGACCTTCTTGCAGCGGGACATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAAGGAACAATACAAACT
AATTCAGCTATTAGCTAACCTTTTGTTAACAGGATTTAACTTGGCTGAGATAATAGCTTTCTTAACAAAAAGTCATTTGA
TTCCCGAGCCCTATGTTCTCCGCATGGAAGAAGCACTCTTAAAAGGTCGAGGGCTGGCAGATATGTTAGCTGGGATAGGT
TTTTCGGATGCTATTTTAACTCAAATTAGTTTAGCAGATGGTCACGGAAACATTGAGACGACGCTTGTTAATATTCAGGG
CTATCTCAACCAAATGGCTAAACTTAAACGAAAAACCATTGAAGTCGTCACCTATCCGATTATTCTCCTGGTATTTCTTT
TTGTGATGATATTAGGGTTAAGGCATTATGTTATGCCTCAATTAGAAGCTCAAAATCAGCTTACTATTTTTCTGACTCAT
TTTCCAGCTATTTTCTTTGGTATTATCCTTTTTATAGGTGTGCTGTTAGGGGGAATTTGGCTGCATTGGCGATCGCAAAG
TCGCCTCAAGCTTTTTACTCACTTCAGTGCTTATCCTGTTTTAGGGAAACTCTTACAACAGTATCTGACGGCTTATTATG
CTAGAGAATGGGGAACATTGATTGGACAGGGACTTGAATTACCAGTCATTCTGGATATTATGGCCATGGAGAAATCTTTA
TTCATGCAAGAATTGGCTGAAGATATTCGAGTTAGTTTATTGGCAGGACAAGCTTTTCATGATAAGGTAGCTACCTATCC
TTTCTTTAAAAAAGAGTTAAGTTTGATGATTACTTATGGAGAGATTAAAACCAAGTTAGGAAGTGAACTAGAGATTTATG
CTCAAGAGAGTTGGACTCAGTTTTTCAGCCAACTTCATCAAGCAACTCAGTTTATTCAACCGATTATCTTTTTAGTGATT
GCTCTGACGATTGTCATGATTTATGCAGCAATTTTATTACCAATTTATCAAAATATGGGAGGCGTTTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-146)

Type II secretion system (T2SS), protein F (T2SSF)

(214-334)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.097

98.834

0.525
  comYB Streptococcus mutans UA159

53.097

98.834

0.525
  comYB Streptococcus gordonii str. Challis substr. CH1

48.378

98.834

0.478
  comGB/cglB Streptococcus mitis NCTC 12261

45.672

97.668

0.446
  comGB/cglB Streptococcus mitis SK321

45.045

97.085

0.437
  comGB/cglB Streptococcus pneumoniae Rx1

43.544

97.085

0.423
  comGB/cglB Streptococcus pneumoniae D39

43.544

97.085

0.423
  comGB/cglB Streptococcus pneumoniae R6

43.544

97.085

0.423
  comGB/cglB Streptococcus pneumoniae TIGR4

43.544

97.085

0.423
  comGB Lactococcus lactis subsp. cremoris KW2

42.773

98.834

0.423

Multiple sequence alignment