Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   EL309_RS05250 Genome accession   NZ_LR134533
Coordinates   1072519..1072998 (-) Length   159 a.a.
NCBI ID   WP_004285215.1    Uniprot ID   -
Organism   Neisseria weaveri strain NCTC12742     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1067519..1077998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL309_RS05220 (NCTC12742_01030) minD 1068133..1068945 (+) 813 WP_004285221.1 septum site-determining protein MinD -
  EL309_RS05225 (NCTC12742_01031) minE 1068949..1069212 (+) 264 WP_004285220.1 cell division topological specificity factor MinE -
  EL309_RS05230 (NCTC12742_01032) - 1069216..1070136 (+) 921 WP_004285219.1 hydrogen peroxide-inducible genes activator -
  EL309_RS05235 (NCTC12742_01033) - 1070316..1070762 (-) 447 WP_004285397.1 CopD family copper resistance protein -
  EL309_RS05240 (NCTC12742_01034) - 1070894..1071097 (+) 204 WP_004285217.1 glycine zipper 2TM domain-containing protein -
  EL309_RS10780 - 1071294..1071683 (-) 390 WP_232014437.1 OmpA family protein -
  EL309_RS10785 - 1071678..1072133 (-) 456 Protein_1017 outer membrane protein assembly factor BamE -
  EL309_RS05250 (NCTC12742_01036) pilE 1072519..1072998 (-) 480 WP_004285215.1 pilin Machinery gene
  EL309_RS05255 (NCTC12742_01037) comE 1074090..1074395 (-) 306 WP_004284769.1 helix-hairpin-helix domain-containing protein Machinery gene

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16673.10 Da        Isoelectric Point: 8.4258

>NTDB_id=1124116 EL309_RS05250 WP_004285215.1 1072519..1072998(-) (pilE) [Neisseria weaveri strain NCTC12742]
MKALQKGFTLIELMIVIAIIGILAVIALPAYQDYTARAQVSEAFSLAEGQKSAVTEYYADKGAWPADNAAAGVAAKADIN
GKYVESVEVSTANSTGYITAKMKGSGVNEDIKGKTLTLKAVKAGGSFKWECDTTKDTTNGIPTKFLPSVCRPDAAKAST

Nucleotide


Download         Length: 480 bp        

>NTDB_id=1124116 EL309_RS05250 WP_004285215.1 1072519..1072998(-) (pilE) [Neisseria weaveri strain NCTC12742]
ATGAAAGCTTTACAAAAAGGTTTTACTTTGATCGAGTTGATGATCGTTATTGCGATTATCGGTATTTTGGCGGTGATTGC
GCTGCCTGCTTACCAAGATTACACAGCGCGTGCACAAGTTTCTGAAGCGTTTTCTTTGGCTGAAGGCCAAAAATCTGCGG
TAACCGAATATTATGCCGATAAAGGTGCATGGCCTGCTGATAATGCAGCGGCAGGTGTTGCTGCTAAAGCTGACATTAAT
GGTAAATATGTTGAATCTGTAGAGGTTTCTACAGCAAATAGCACAGGCTACATTACTGCAAAGATGAAAGGCAGCGGTGT
AAATGAAGATATTAAAGGTAAAACTTTAACCTTAAAAGCAGTAAAAGCCGGCGGTTCATTTAAGTGGGAATGTGATACTA
CAAAGGATACAACTAATGGTATTCCAACGAAATTCCTGCCATCTGTTTGCCGTCCTGATGCTGCTAAAGCTTCTACTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)

Pilin (bacterial filament) (Pilin)

(36-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

59.763

100

0.635
  pilE Neisseria gonorrhoeae strain FA1090

58.434

100

0.61
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.741

100

0.484
  pilA/pilA1 Eikenella corrodens VA1

45

100

0.453
  comP Acinetobacter baylyi ADP1

42.581

97.484

0.415
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.373

100

0.409
  pilA2 Legionella pneumophila str. Paris

43.624

93.711

0.409
  pilA2 Legionella pneumophila strain ERS1305867

43.624

93.711

0.409
  pilA Acinetobacter baumannii strain A118

39.865

93.082

0.371

Multiple sequence alignment