Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL215_RS00260 Genome accession   NZ_LR134481
Coordinates   50509..51891 (+) Length   460 a.a.
NCBI ID   WP_126469443.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain NCTC10665     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 45509..56891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL215_RS00225 (NCTC10665_00044) - 45808..46659 (-) 852 WP_126469431.1 thymidylate synthase -
  EL215_RS00230 (NCTC10665_00045) lgt 46670..47479 (-) 810 WP_126469433.1 prolipoprotein diacylglyceryl transferase -
  EL215_RS00235 (NCTC10665_00046) - 47488..48282 (-) 795 WP_126469435.1 sulfite exporter TauE/SafE family protein -
  EL215_RS00240 (NCTC10665_00047) rppH 48413..48875 (-) 463 Protein_46 RNA pyrophosphohydrolase -
  EL215_RS00250 (NCTC10665_00048) ampD 49360..49923 (-) 564 WP_126469439.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL215_RS00255 (NCTC10665_00049) pilA 50065..50502 (+) 438 WP_126469441.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EL215_RS00260 (NCTC10665_00050) pilB 50509..51891 (+) 1383 WP_126469443.1 GspE/PulE family protein Machinery gene
  EL215_RS00265 (NCTC10665_00051) pilC 51900..53123 (+) 1224 WP_126469445.1 type II secretion system F family protein Machinery gene
  EL215_RS00270 (NCTC10665_00052) pilD 53120..53797 (+) 678 WP_126469447.1 prepilin peptidase Machinery gene
  EL215_RS00275 (NCTC10665_00053) coaE 53883..54503 (+) 621 WP_126469449.1 dephospho-CoA kinase -
  EL215_RS00280 (NCTC10665_00054) yacG 54496..54702 (+) 207 WP_126469451.1 DNA gyrase inhibitor YacG -
  EL215_RS00285 (NCTC10665_00055) - 54699..54980 (+) 282 WP_126469453.1 GNAT family N-acetyltransferase -
  EL215_RS00290 (NCTC10665_00056) rhlB 55071..56330 (+) 1260 WP_126469455.1 ATP-dependent RNA helicase RhlB -
  EL215_RS00295 (NCTC10665_00057) mreD 56371..56859 (-) 489 WP_126469457.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 52181.55 Da        Isoelectric Point: 6.0181

>NTDB_id=1123022 EL215_RS00260 WP_126469443.1 50509..51891(+) (pilB) [Haemophilus parainfluenzae strain NCTC10665]
MAYQAIAKNGEICQISPQYWQQNQQQQALLLRYFALPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETHQLK
QLLQSLAPKANQIIEEQTTFYHHSEDENTANISNDDEPVIQLLNGIFETALQKNASDIHLETQPSGLLIRLRIDGVLQPQ
PIISKALANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTK
NQQRQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFL
RQDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDALSAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCSQH
FSESCDCHQGYQGRIGVYQFLQCENNCYQTDFDSLYQSALEKVKDNITDLDEIQRVLGKK

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=1123022 EL215_RS00260 WP_126469443.1 50509..51891(+) (pilB) [Haemophilus parainfluenzae strain NCTC10665]
ATGGCCTATCAAGCAATCGCAAAAAATGGCGAGATTTGCCAAATCTCGCCACAATATTGGCAACAAAACCAACAGCAGCA
AGCTTTACTGCTGCGTTACTTTGCGCTACCACTTAAAGAAGACGAGCACCATTTATGGCTTGCAGTGGATTCACTCAATA
ACCTTGCGGCTTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTAGTTGAACCGATTTTATTTGAAACACATCAACTCAAA
CAACTTTTACAATCCCTTGCACCTAAAGCAAATCAAATAATAGAAGAGCAAACAACGTTTTATCATCATTCAGAAGACGA
AAATACTGCCAATATATCTAATGACGATGAACCTGTTATTCAATTATTAAATGGTATTTTTGAAACAGCCCTGCAAAAAA
ATGCCTCCGATATTCATTTAGAAACGCAGCCAAGTGGGCTCTTAATTCGTTTACGTATTGATGGCGTACTACAACCACAG
CCTATTATCAGTAAAGCACTCGCCAATCGTGTGATTTCTCGTTTAAAACTCTTAGCCAAATTGGATATCAGTGAAACTCG
ATTGCCACAAGATGGCCGCTTTCAATTCAAAACCACGTTTTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCTACTC
ATTGGGGAGAAAAAGCGGTATTACGATTACAACAAAATAAACCTGTTCAGTTAAGTTTTGCTGAACTTGGCATGACAAAA
AACCAACAACGTCAATTTCAACATGCACTCAGCCAACCACAAGGTTTAATCCTCGTGACTGGACCAACGGGAAGCGGAAA
AAGTATCTCGCTTTATACCGCACTTCAATGGCTCAATACGCCAGATAAACATATTATGACGGCAGAGGATCCCGTTGAAA
TTGAATTAGAAGGCATTATTCAAACTCAAGTGAATCCGCAAATTGGTTTAGATTTTAGCCGATTACTTCGTACATTTTTA
CGACAAGACCCTGACATTATTATGCTTGGTGAAATTCGAGATGAAGAAAGTGCATTAATGGCATTAAGAGCAGCTCAAAC
CGGCCATTTAGTGCTTTCGACCTTACATACCAACGATGCCCTCTCAGCCATTTCACGCTTGCAACAACTAGGCATTCAAT
CTCACGAAATTGAAAATAGCCTCTTATTAGTTATCGCACAGCGATTGGTGCGTAAACGTTGCTTAAAGTGCAGTCAACAT
TTTAGTGAGTCTTGCGATTGCCATCAGGGGTATCAAGGGCGTATTGGGGTGTATCAATTTTTACAATGTGAAAATAACTG
TTACCAAACTGATTTCGATTCACTTTATCAAAGTGCATTAGAAAAAGTAAAAGATAACATCACCGATCTTGATGAAATTC
AACGTGTGTTAGGTAAAAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(126-392)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-88)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

77.925

98.478

0.767
  pilB Haemophilus influenzae Rd KW20

76.754

99.13

0.761
  pilB Glaesserella parasuis strain SC1401

60.044

98.478

0.591
  pilB Vibrio campbellii strain DS40M4

40.825

100

0.43
  pilB Vibrio cholerae strain A1552

41.25

100

0.43
  pilB Vibrio parahaemolyticus RIMD 2210633

40.748

100

0.426
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.417
  pilB Acinetobacter baumannii D1279779

39.264

100

0.417
  pilB Acinetobacter baylyi ADP1

37.876

100

0.411
  pilF Neisseria gonorrhoeae MS11

37.161

100

0.387
  pilF Thermus thermophilus HB27

38.074

99.348

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.095

100

0.361

Multiple sequence alignment