Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EL215_RS00255 Genome accession   NZ_LR134481
Coordinates   50065..50502 (+) Length   145 a.a.
NCBI ID   WP_126469441.1    Uniprot ID   A0A3S4WQI0
Organism   Haemophilus parainfluenzae strain NCTC10665     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 45065..55502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL215_RS00220 (NCTC10665_00043) tadA 45280..45798 (-) 519 WP_126469429.1 tRNA adenosine(34) deaminase TadA -
  EL215_RS00225 (NCTC10665_00044) - 45808..46659 (-) 852 WP_126469431.1 thymidylate synthase -
  EL215_RS00230 (NCTC10665_00045) lgt 46670..47479 (-) 810 WP_126469433.1 prolipoprotein diacylglyceryl transferase -
  EL215_RS00235 (NCTC10665_00046) - 47488..48282 (-) 795 WP_126469435.1 sulfite exporter TauE/SafE family protein -
  EL215_RS00240 (NCTC10665_00047) rppH 48413..48875 (-) 463 Protein_46 RNA pyrophosphohydrolase -
  EL215_RS00250 (NCTC10665_00048) ampD 49360..49923 (-) 564 WP_126469439.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL215_RS00255 (NCTC10665_00049) pilA 50065..50502 (+) 438 WP_126469441.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EL215_RS00260 (NCTC10665_00050) pilB 50509..51891 (+) 1383 WP_126469443.1 GspE/PulE family protein Machinery gene
  EL215_RS00265 (NCTC10665_00051) pilC 51900..53123 (+) 1224 WP_126469445.1 type II secretion system F family protein Machinery gene
  EL215_RS00270 (NCTC10665_00052) pilD 53120..53797 (+) 678 WP_126469447.1 prepilin peptidase Machinery gene
  EL215_RS00275 (NCTC10665_00053) coaE 53883..54503 (+) 621 WP_126469449.1 dephospho-CoA kinase -
  EL215_RS00280 (NCTC10665_00054) yacG 54496..54702 (+) 207 WP_126469451.1 DNA gyrase inhibitor YacG -
  EL215_RS00285 (NCTC10665_00055) - 54699..54980 (+) 282 WP_126469453.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15041.22 Da        Isoelectric Point: 8.1030

>NTDB_id=1123021 EL215_RS00255 WP_126469441.1 50065..50502(+) (pilA) [Haemophilus parainfluenzae strain NCTC10665]
MKLTFSKPLHKGFTLIELMIVIIAILATIAIPSYQNYTKKAAISELLQASAPYKSDVELCVYSTNSPTNCSGGSNGIAAD
ITTAKGYVKSITTKSGVITVTGNDTLDGISYSLTATGSTSSGVTWATACPSNADLFPAGFCSPTK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1123021 EL215_RS00255 WP_126469441.1 50065..50502(+) (pilA) [Haemophilus parainfluenzae strain NCTC10665]
ATGAAACTGACTTTTTCTAAACCTTTACATAAAGGTTTTACGTTAATTGAATTGATGATCGTGATTATTGCTATTCTCGC
GACGATCGCTATTCCGTCTTATCAAAATTATACCAAAAAAGCAGCCATCTCAGAATTGTTGCAAGCTTCGGCACCTTATA
AATCTGATGTAGAGTTATGCGTCTATAGCACCAATTCCCCAACAAACTGTTCTGGTGGTTCAAATGGTATTGCGGCAGAC
ATCACTACCGCAAAAGGCTATGTTAAATCCATTACCACGAAATCGGGAGTGATTACGGTAACAGGAAATGACACATTGGA
TGGGATCAGTTATTCTTTAACGGCGACAGGCTCAACCTCATCAGGTGTCACTTGGGCAACCGCTTGTCCAAGCAATGCGG
ATTTATTCCCTGCGGGTTTCTGCTCTCCTACTAAATAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4WQI0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

68.75

99.31

0.683
  pilA Haemophilus influenzae Rd KW20

66.667

99.31

0.662
  pilA Glaesserella parasuis strain SC1401

50

100

0.51
  pilE Neisseria gonorrhoeae MS11

40.458

90.345

0.366
  pilA Vibrio campbellii strain DS40M4

36.301

100

0.366

Multiple sequence alignment