Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EL215_RS00270 Genome accession   NZ_LR134481
Coordinates   53120..53797 (+) Length   225 a.a.
NCBI ID   WP_126469447.1    Uniprot ID   A0A3S4XFB3
Organism   Haemophilus parainfluenzae strain NCTC10665     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 48120..58797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL215_RS00240 (NCTC10665_00047) rppH 48413..48875 (-) 463 Protein_46 RNA pyrophosphohydrolase -
  EL215_RS00250 (NCTC10665_00048) ampD 49360..49923 (-) 564 WP_126469439.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EL215_RS00255 (NCTC10665_00049) pilA 50065..50502 (+) 438 WP_126469441.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EL215_RS00260 (NCTC10665_00050) pilB 50509..51891 (+) 1383 WP_126469443.1 GspE/PulE family protein Machinery gene
  EL215_RS00265 (NCTC10665_00051) pilC 51900..53123 (+) 1224 WP_126469445.1 type II secretion system F family protein Machinery gene
  EL215_RS00270 (NCTC10665_00052) pilD 53120..53797 (+) 678 WP_126469447.1 prepilin peptidase Machinery gene
  EL215_RS00275 (NCTC10665_00053) coaE 53883..54503 (+) 621 WP_126469449.1 dephospho-CoA kinase -
  EL215_RS00280 (NCTC10665_00054) yacG 54496..54702 (+) 207 WP_126469451.1 DNA gyrase inhibitor YacG -
  EL215_RS00285 (NCTC10665_00055) - 54699..54980 (+) 282 WP_126469453.1 GNAT family N-acetyltransferase -
  EL215_RS00290 (NCTC10665_00056) rhlB 55071..56330 (+) 1260 WP_126469455.1 ATP-dependent RNA helicase RhlB -
  EL215_RS00295 (NCTC10665_00057) mreD 56371..56859 (-) 489 WP_126469457.1 rod shape-determining protein MreD -
  EL215_RS00300 (NCTC10665_00058) mreC 56859..57923 (-) 1065 WP_049357720.1 rod shape-determining protein MreC -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 26188.31 Da        Isoelectric Point: 7.6984

>NTDB_id=1123024 EL215_RS00270 WP_126469447.1 53120..53797(+) (pilD) [Haemophilus parainfluenzae strain NCTC10665]
MMILAFFLLGGLAGIFVWLYIDRFIPNLQQEIYQNYIELYPENRYLFHSKKTAIKSQKCGHILLYFLGFGLCFAVLYYFL
QDELFTLWLAITLSLLFVISWLDWHYQLISPTPCLFLFFLGLFGAHQEFSILTLSQSLESAVSFFSVFYIIYHLSKWFYK
KEALGRGDYWLALGIGAYLTIAHLPLFLFIACLLGIIVYWISRKPVLPFAPFLCLSLIITSAITL

Nucleotide


Download         Length: 678 bp        

>NTDB_id=1123024 EL215_RS00270 WP_126469447.1 53120..53797(+) (pilD) [Haemophilus parainfluenzae strain NCTC10665]
ATGATGATATTAGCCTTTTTCTTATTGGGTGGTTTAGCGGGTATCTTTGTTTGGCTTTATATTGATCGTTTTATCCCCAA
TCTCCAACAAGAAATTTATCAGAACTATATTGAACTTTATCCTGAAAATAGGTACTTATTTCACAGCAAAAAAACAGCAA
TTAAATCTCAAAAGTGCGGTCATATTTTGCTTTATTTTTTAGGGTTTGGACTCTGTTTTGCTGTACTCTATTATTTTCTT
CAGGATGAATTATTTACTTTATGGCTAGCAATTACGCTTAGTCTACTTTTTGTTATCAGTTGGCTAGACTGGCATTATCA
ACTGATTTCACCTACACCTTGCCTCTTTTTATTTTTCCTAGGTTTATTCGGCGCACACCAAGAGTTTTCAATCCTGACAC
TTTCTCAAAGTTTAGAAAGTGCGGTTAGTTTTTTCAGTGTTTTTTATATTATCTATCATTTGTCTAAATGGTTTTATAAG
AAGGAAGCATTAGGCCGTGGTGATTATTGGCTTGCTTTAGGAATAGGTGCTTATCTCACCATCGCACACTTACCACTTTT
CTTATTCATCGCCTGTCTATTAGGTATCATAGTCTATTGGATTTCTCGCAAACCTGTTTTACCTTTTGCGCCCTTTCTTT
GCTTATCTTTAATCATCACCAGTGCAATAACCCTATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(91-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4XFB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae Rd KW20

52.941

98.222

0.52
  pilD Haemophilus influenzae 86-028NP

52.036

98.222

0.511

Multiple sequence alignment