Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL255_RS12840 Genome accession   NZ_LR134376
Coordinates   2804905..2806044 (+) Length   379 a.a.
NCBI ID   WP_042654691.1    Uniprot ID   A0AAE9MJZ6
Organism   Aeromonas encheleia strain NCTC12917     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2799905..2811044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL255_RS12815 (NCTC12917_02564) - 2800335..2801402 (+) 1068 WP_042654696.1 3-deoxy-7-phosphoheptulonate synthase -
  EL255_RS12820 (NCTC12917_02565) - 2801791..2802570 (-) 780 WP_042654695.1 ATP-binding cassette domain-containing protein -
  EL255_RS12825 (NCTC12917_02566) btuC 2802557..2803558 (-) 1002 WP_042654694.1 vitamin B12 ABC transporter permease BtuC -
  EL255_RS12830 (NCTC12917_02567) - 2803646..2804377 (-) 732 WP_042654693.1 ABC transporter substrate-binding protein -
  EL255_RS12835 (NCTC12917_02568) - 2804490..2804846 (+) 357 WP_042654692.1 DMT family protein -
  EL255_RS12840 (NCTC12917_02569) pilU 2804905..2806044 (+) 1140 WP_042654691.1 type IVa pilus ATPase TapW Machinery gene
  EL255_RS12845 (NCTC12917_02570) glgC 2806307..2807578 (+) 1272 WP_042654690.1 glucose-1-phosphate adenylyltransferase -
  EL255_RS12850 (NCTC12917_02571) - 2807672..2807914 (-) 243 WP_042654689.1 FeoC-like transcriptional regulator -
  EL255_RS12855 (NCTC12917_02572) feoB 2807940..2810210 (-) 2271 WP_042654688.1 Fe(2+) transporter permease subunit FeoB -
  EL255_RS12860 (NCTC12917_02573) - 2810263..2810490 (-) 228 WP_042654687.1 FeoA family protein -
  EL255_RS12865 (NCTC12917_02574) - 2810610..2810939 (+) 330 WP_042654686.1 translation initiation factor -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42337.22 Da        Isoelectric Point: 6.1970

>NTDB_id=1122441 EL255_RS12840 WP_042654691.1 2804905..2806044(+) (pilU) [Aeromonas encheleia strain NCTC12917]
MELRDMLQTLASQDGSDLYLSTGAPPCAKFNGALRPLSETSLEPGEVARIAADLMDSEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNHSGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTIRDLIKRGEFGSLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWQEKGSLANSADASGWSLEPTKDDKDELF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1122441 EL255_RS12840 WP_042654691.1 2804905..2806044(+) (pilU) [Aeromonas encheleia strain NCTC12917]
ATGGAATTGCGAGACATGCTACAGACGCTGGCCAGCCAGGATGGCTCAGACCTCTACCTCTCCACCGGTGCGCCGCCCTG
CGCCAAGTTCAACGGCGCCCTGCGCCCCCTGAGCGAGACCAGCCTGGAGCCGGGTGAGGTGGCCAGGATCGCCGCCGATC
TCATGGACTCGGAGCAGAAGCAGCAGTTCGAGAAGGAGCTGGAGATGAATCTCGCCATCTCCCTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAGGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCGCGCTTCGA
GGATCTCAAGCTGCCCCCTGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGCACCGGCT
CCGGCAAGTCCACCTCGCTGGCGGCGCTGATCGACCATCGCAACCGCAACCACAGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAGACCGGGCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAGGAGCGCCGCCCCCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
GCTGGTGAAGACCACGGATGGCGGCCGGCGGGCGGCGGTGGAGGTCATGCTCGGCACCCACACCATCCGCGACCTCATCA
AGCGTGGCGAGTTTGGCAGCCTCAAGGAGATCATGGAGAAATCCAGGGCGCTCGGCATGGTCACCTTCGACAGCACCCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCGGCCAACAACTTGCGCCTCAA
GATCAAGCTGTGGCAGGAGAAGGGCTCCCTCGCCAACAGTGCGGATGCCAGCGGCTGGAGTCTGGAGCCCACCAAGGATG
ACAAGGACGAGCTGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.684

100

0.538
  pilU Acinetobacter baylyi ADP1

52.394

93.668

0.491
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.51

93.14

0.377
  pilT Pseudomonas aeruginosa PAK

40.116

90.765

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361

Multiple sequence alignment