Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EL257_RS22965 Genome accession   NZ_LR134318
Coordinates   5049578..5050795 (+) Length   405 a.a.
NCBI ID   WP_126366473.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain NCTC9428     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5044578..5055795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL257_RS22945 (NCTC9428_04577) - 5046021..5046320 (+) 300 WP_126366465.1 DUF2845 domain-containing protein -
  EL257_RS22950 (NCTC9428_04578) - 5046583..5046936 (-) 354 WP_126366467.1 BON domain-containing protein -
  EL257_RS22955 (NCTC9428_04579) - 5047233..5047649 (-) 417 WP_126366469.1 pilin -
  EL257_RS22960 (NCTC9428_04580) pilB 5047875..5049575 (+) 1701 WP_126366471.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL257_RS22965 (NCTC9428_04581) pilC 5049578..5050795 (+) 1218 WP_126366473.1 type II secretion system F family protein Machinery gene
  EL257_RS22970 (NCTC9428_04582) pilD 5050797..5051669 (+) 873 WP_126366475.1 prepilin peptidase Machinery gene
  EL257_RS22975 (NCTC9428_04583) coaE 5051666..5052289 (+) 624 WP_126366477.1 dephospho-CoA kinase -
  EL257_RS22980 (NCTC9428_04584) yacG 5052286..5052486 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  EL257_RS22985 (NCTC9428_04585) - 5052519..5053208 (-) 690 WP_126366479.1 energy-coupling factor ABC transporter permease -
  EL257_RS22995 (NCTC9428_04586) - 5053397..5054023 (+) 627 WP_126366481.1 DUF1780 domain-containing protein -
  EL257_RS23000 (NCTC9428_04587) - 5054020..5054547 (+) 528 WP_126366483.1 MOSC domain-containing protein -
  EL257_RS23005 (NCTC9428_04588) - 5054599..5054772 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44009.81 Da        Isoelectric Point: 10.1769

>NTDB_id=1121328 EL257_RS22965 WP_126366473.1 5049578..5050795(+) (pilC) [Pseudomonas fluorescens strain NCTC9428]
MAVKAAKMNVYAWEGTDRKGSKISGELSGVNPALVKAQLRKQGINPGKVRKKSASLLTLGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ERLKAKIKKAMTYPTAVILVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISLSHFMQDWWLALLGVLAVAIFGTR
HALKKSQALRDRRDTWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEEEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1121328 EL257_RS22965 WP_126366473.1 5049578..5050795(+) (pilC) [Pseudomonas fluorescens strain NCTC9428]
ATGGCGGTCAAGGCAGCGAAAATGAACGTCTACGCCTGGGAAGGCACGGACAGGAAAGGCAGCAAAATCAGCGGCGAACT
CAGCGGCGTCAATCCCGCACTGGTCAAGGCGCAGCTACGCAAACAGGGAATCAACCCCGGTAAGGTGCGCAAGAAATCCG
CGTCGTTGCTGACCTTGGGCAAACGCATCAAGGCGCAAGACATCGCCCTGTTCACCCGGCAAATGGCGACGATGATGAAG
GCTGGCGTGCCGCTGTTGCAGTCGTTCGACATCATCGGTGAAGGCTTCGATAACCCGGCCATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTGGCCGCCGGTAACAGCTTCGCCGCGGCACTGCGCAAGAAGCCGCAATATTTCGACGAATTGTATT
GCAACCTGGTCGATGCCGGCGAGCAGTCCGGCGCCCTCGATACGCTGCTGGAACGGGTCGCGACCTACAAGGAAAAAAGC
GAACGGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCTACCGCCGTGATCCTGGTGGCGGCCGTGGTGACCGGGAT
TCTGCTGGTCAAAGTGGTGCCGCAGTTCCAATCGGTTTTTTCCGGCTTCGGTGCCGAGTTACCCGCGTTCACCTTGATGG
TCATCAGCCTGTCGCACTTCATGCAGGATTGGTGGCTGGCGCTGCTCGGCGTGCTGGCGGTGGCAATTTTCGGCACTCGT
CATGCACTGAAAAAATCCCAGGCCTTGCGCGACCGCCGCGACACCTGGCTGCTGAAACTGCCCTTGGTCGGCACCCTGAT
GTACAAATCCGCCGTGGCGCGCTTCGCCCGCACCCTGTCGACGACGTTCGCCGCCGGCGTGCCGCTGGTCGAAGCGCTCG
ACTCGGTGGCCGGCGCCACCGGCAACGTGGTTTTCAAACGCGCCGTGCTGCGTGTTCGCCAGGATGTATCCACAGGCATG
CAGCTGAACTTTTCAATGCGCAGCACCGGCGTCTTTCCGAACATGGCGGTGCAAATGACCGCGATCGGCGAAGAGTCCGG
TGCACTGGACGATATGCTCGACAAGGTCGCTGGTTTCTATGAGGAAGAAGTGGATAACATGGTCGACAACCTCACCAGCC
TCATGGAGCCGTTCATCATGGTTGTGCTGGGGGTAATCGTCGGCGGATTGGTCGTGGCGATGTACCTGCCGATTTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baylyi ADP1

60.099

100

0.602
  pilC Acinetobacter baumannii D1279779

59.069

100

0.595
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilC Vibrio cholerae strain A1552

44.691

100

0.447
  pilG Neisseria gonorrhoeae MS11

43.069

99.753

0.43
  pilG Neisseria meningitidis 44/76-A

42.574

99.753

0.425
  pilC Vibrio campbellii strain DS40M4

42.26

100

0.425
  pilC Thermus thermophilus HB27

38.903

99.012

0.385

Multiple sequence alignment