Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL257_RS22960 Genome accession   NZ_LR134318
Coordinates   5047875..5049575 (+) Length   566 a.a.
NCBI ID   WP_126366471.1    Uniprot ID   A0A3S4SX90
Organism   Pseudomonas fluorescens strain NCTC9428     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5042875..5054575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL257_RS22940 (NCTC9428_04576) - 5043073..5046024 (+) 2952 WP_126366463.1 DUF748 domain-containing protein -
  EL257_RS22945 (NCTC9428_04577) - 5046021..5046320 (+) 300 WP_126366465.1 DUF2845 domain-containing protein -
  EL257_RS22950 (NCTC9428_04578) - 5046583..5046936 (-) 354 WP_126366467.1 BON domain-containing protein -
  EL257_RS22955 (NCTC9428_04579) - 5047233..5047649 (-) 417 WP_126366469.1 pilin -
  EL257_RS22960 (NCTC9428_04580) pilB 5047875..5049575 (+) 1701 WP_126366471.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL257_RS22965 (NCTC9428_04581) pilC 5049578..5050795 (+) 1218 WP_126366473.1 type II secretion system F family protein Machinery gene
  EL257_RS22970 (NCTC9428_04582) pilD 5050797..5051669 (+) 873 WP_126366475.1 prepilin peptidase Machinery gene
  EL257_RS22975 (NCTC9428_04583) coaE 5051666..5052289 (+) 624 WP_126366477.1 dephospho-CoA kinase -
  EL257_RS22980 (NCTC9428_04584) yacG 5052286..5052486 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  EL257_RS22985 (NCTC9428_04585) - 5052519..5053208 (-) 690 WP_126366479.1 energy-coupling factor ABC transporter permease -
  EL257_RS22995 (NCTC9428_04586) - 5053397..5054023 (+) 627 WP_126366481.1 DUF1780 domain-containing protein -
  EL257_RS23000 (NCTC9428_04587) - 5054020..5054547 (+) 528 WP_126366483.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62663.59 Da        Isoelectric Point: 6.0449

>NTDB_id=1121327 EL257_RS22960 WP_126366471.1 5047875..5049575(+) (pilB) [Pseudomonas fluorescens strain NCTC9428]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRSRQSLVSYLVQNKLVKGWQVAEIASEHFGMAFLDLQCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
LDVESIDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPHDTLTREGFPE
ERIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTQALQRLIMAEGNSLEIDTQMRRDGFDDLRTAGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1121327 EL257_RS22960 WP_126366471.1 5047875..5049575(+) (pilB) [Pseudomonas fluorescens strain NCTC9428]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTCACGGAAAAAAGCGCCCAGCAAGC
CTGGCAGCAGGCTCAACGCAGTCGCCAGTCGCTGGTCAGCTATCTGGTGCAAAACAAACTGGTGAAAGGCTGGCAGGTCG
CCGAAATCGCTTCGGAGCATTTCGGCATGGCCTTTCTTGACCTTCAATGTCTGGACAAAGAGACCCAGCCCAAAGGCCTG
GTCAGCGAAAAGCTTGTGCGCCAGCATCACGCCCTGCCCTTGTGGCGGCGCGGCAACAAGCTGTTCGTCGGCATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATCCAGTTCAGCACCGGGCTAAGCACCGAAGCCATTCTGGTGGAAGACGACA
AGCTCACCGATGCCATCGAAAAATTCTTCGACACCCACGCCAGCGGTCTCGAGGACATGGCCGATGTCGACCTCGACGGC
CTCGATGTCGAATCCATCGACGACAGCAAACAGGACGCTATCGGTGGCCTCGACGCCGACGATGCGCCGGTGGTGCGCTT
CGTCCACAAGATGTTGCTGGACGCGATCAAAAGCGGCTCGTCCGACCTGCACTTCGAACCGTACGAAAAAAACTACCGGG
TCCGGATGCGCACCGATGGCATGTTGCGCGAGGTGGCCAAGCCGCCGATCCAGTTGGCCGGACGCATCGCCGCGCGCCTG
AAAGTCATGGCCAGCCTCGATATCTCGGAGCGCCGCAAGCCGCAGGACGGCCGGATCAAGATGCGTCTGTCGAAAAGCAA
GTCGATCGATTTCCGCGTCAACACCTTGCCGACTCTGTGGGGTGAGAAAGTGGTGATCCGGATTCTCGACCCGTCGAGTG
CGCAGATGGGTATCGATGCCTTGGGTTACGAGCCGGAGCAAAAAGCCCTGTATCTGGCCGCGCTCAAACAACCCCAGGGA
ATGATTCTGGTCACTGGCCCCACCGGCTCTGGCAAGACGGTGTCGCTGTACACCGGGCTGAATATTCTCAACACCGTCGA
CATCAATATTTCTACCGCCGAAGACCCGGTGGAAATCAATATGGAGGGCATCAACCAGGTCAACGTCAATCCCCGCCAAG
GCCTCGACTTCGCCCAGGCCCTGCGCTCGTTTCTGCGACAGGACCCGGACGTGATCATGGTTGGCGAGATTCGTGACCTG
GAAACCGCCGAGATCGCCATCAAAGCGGCGCAGACCGGTCACCTCGTACTCTCCACCCTGCACACCAACAGCGCGGCGGA
AACCCTTACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATTGCCACTTCGGTCAGCCTGATCATTGCGCAGCGAC
TGGCGCGCAAGCTCTGCAGCCATTGCAAAAAGCCCATCGAGATTCCGCACGACACGCTGACCCGCGAAGGCTTCCCTGAG
GAACGCATCGGCAGTTTCACGATCTACGAACCGGTCGGCTGCGATCACTGCAACGGCGGATACAAAGGACGCGTAGGTAT
CTATGAAGTGGTGAAAAACACTCAGGCGCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAAATCGACACGCAGA
TGCGCCGCGACGGTTTCGACGATCTGCGCACCGCCGGGCTGCACAAAGCCATGCAAGGCATTACCAGCCTTGAAGAAATC
AATCGGGTTACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4SX90

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.053

99.823

0.58
  pilB Acinetobacter baumannii D1279779

57.624

99.647

0.574
  pilB Legionella pneumophila strain ERS1305867

55.477

100

0.555
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.554

99.117

0.491
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.015

93.286

0.364
  pilB/pilB1 Synechocystis sp. PCC 6803

39.122

92.58

0.362
  pilF Thermus thermophilus HB27

36.738

98.587

0.362

Multiple sequence alignment