Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   EL070_RS00680 Genome accession   NZ_LR134284
Coordinates   104386..105324 (+) Length   312 a.a.
NCBI ID   WP_002987773.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8232     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99386..110324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL070_RS00670 (NCTC8232_00133) rpoC 100135..103776 (+) 3642 WP_126405105.1 DNA-directed RNA polymerase subunit beta' -
  EL070_RS00675 (NCTC8232_00134) - 103928..104293 (+) 366 WP_111705425.1 DUF1033 family protein -
  EL070_RS00680 (NCTC8232_00135) comYA 104386..105324 (+) 939 WP_002987773.1 competence type IV pilus ATPase ComGA Machinery gene
  EL070_RS00685 (NCTC8232_00136) comYB 105260..106294 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL070_RS00690 (NCTC8232_00137) comYC 106296..106622 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  EL070_RS00695 (NCTC8232_00138) comGD 106597..107025 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EL070_RS00700 (NCTC8232_00139) comGE 106982..107266 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  EL070_RS00705 (NCTC8232_00140) comYF 107259..107693 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL070_RS00710 (NCTC8232_00141) comGG 107677..108003 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  EL070_RS00715 (NCTC8232_00142) comYH 108101..109054 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  EL070_RS00720 (NCTC8232_00143) - 109113..110309 (+) 1197 WP_002986533.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35046.30 Da        Isoelectric Point: 8.5799

>NTDB_id=1120077 EL070_RS00680 WP_002987773.1 104386..105324(+) (comYA) [Streptococcus pyogenes strain NCTC8232]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGITKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1120077 EL070_RS00680 WP_002987773.1 104386..105324(+) (comYA) [Streptococcus pyogenes strain NCTC8232]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAAGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAGTTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAAATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTATAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

61.736

99.679

0.615
  comYA Streptococcus mutans UA140

61.736

99.679

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

60.45

99.679

0.603
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.359

0.599
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

99.679

0.587
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

40

92.949

0.372

Multiple sequence alignment