Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   EL070_RS00715 Genome accession   NZ_LR134284
Coordinates   108101..109054 (+) Length   317 a.a.
NCBI ID   WP_002987790.1    Uniprot ID   A0A5S4TCX5
Organism   Streptococcus pyogenes strain NCTC8232     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 103101..114054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL070_RS00675 (NCTC8232_00134) - 103928..104293 (+) 366 WP_111705425.1 DUF1033 family protein -
  EL070_RS00680 (NCTC8232_00135) comYA 104386..105324 (+) 939 WP_002987773.1 competence type IV pilus ATPase ComGA Machinery gene
  EL070_RS00685 (NCTC8232_00136) comYB 105260..106294 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL070_RS00690 (NCTC8232_00137) comYC 106296..106622 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  EL070_RS00695 (NCTC8232_00138) comGD 106597..107025 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EL070_RS00700 (NCTC8232_00139) comGE 106982..107266 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  EL070_RS00705 (NCTC8232_00140) comYF 107259..107693 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL070_RS00710 (NCTC8232_00141) comGG 107677..108003 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  EL070_RS00715 (NCTC8232_00142) comYH 108101..109054 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  EL070_RS00720 (NCTC8232_00143) - 109113..110309 (+) 1197 WP_002986533.1 acetate kinase -
  EL070_RS00725 - 110496..110804 (+) 309 Protein_92 hypothetical protein -
  EL070_RS00730 (NCTC8232_00144) proC 110887..111657 (-) 771 WP_111705426.1 pyrroline-5-carboxylate reductase -
  EL070_RS00735 (NCTC8232_00145) pepA 111705..112772 (-) 1068 WP_031488658.1 glutamyl aminopeptidase -
  EL070_RS00745 (NCTC8232_00146) - 113237..113521 (+) 285 WP_011888537.1 DUF4651 domain-containing protein -
  EL070_RS00750 (NCTC8232_00147) - 113518..113835 (+) 318 WP_002986515.1 thioredoxin family protein -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 35985.08 Da        Isoelectric Point: 4.7205

>NTDB_id=1120081 EL070_RS00715 WP_002987790.1 108101..109054(+) (comYH) [Streptococcus pyogenes strain NCTC8232]
MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEGASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQT
EQLQANHQFTPDAIGFILLYLLEQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSLKYLKKDGFAIFLAPVNLLTSPQ
SQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIVLQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN

Nucleotide


Download         Length: 954 bp        

>NTDB_id=1120081 EL070_RS00715 WP_002987790.1 108101..109054(+) (comYH) [Streptococcus pyogenes strain NCTC8232]
ATGACTTTTGAAAAAATTGAAGAAGCTTATCAGCTGCTCTTAGAGAACTGTCAACTAATTGAAAATGATCTAAAAACACA
TATATACGATGCCATTGTTGAACAAAACTCCTTTTATTTAGGGGCTGAGGGAGCTAGTCCTCAAGTTGCTCAAAATAGTG
ATAAACTGAAAGCCTTGTGCCTGACAAAAGAAGAATGGCGTAAGGCCTACCAGTTTCTTTTTATTAAGGCAGCTCAGACT
GAGCAACTCCAAGCCAACCATCAGTTCACACCAGATGCTATTGGCTTCATTCTGCTGTATCTTTTGGAACAATTGAGTGA
TAAAGATAGCTTAGAGGTACTTGAGATTGGAAGTGGAACAGGGAACCTAGCCCAAACCCTTCTCAACAACACGAGCAAGA
GCCTTGATTATGTAGGGATTGAACTTGATGATCTCTTGATTGATCTGTCAGCCAGTATTGCTGAGATAATGGATTCTTCA
GCCCATTTTATTCAAGAAGATGCGGTAAGGCCTCAATTACTAAAAGAAAGTGACATTGTCATCAGTGACTTACCAGTTGG
TTATTATCCTAACGATGATATTGCCAAACGGTACAAGGTGGCTAGTTCAGATAAGCATACCTATGCCCATCATTTATTAA
TGGAACAGTCTTTAAAATACTTGAAAAAAGACGGTTTTGCGATTTTTCTGGCACCAGTCAATTTATTGACGAGCCCTCAG
AGCCAGTTATTGAAACAGTGGTTAAAAGATTATGCTCAGGTGGTGGCCTTGATTACGCTACCAGATTCTATTTTTGGTCA
TCCCTCTAATGCTAAGTCCATTATTGTCTTACAAAAACAAACAGACCACCCAATGGAAACCTTTGTCTATCCAATTCGGG
ATTTGAAGCTTGCAGAGAATATTCATGATTTTATGGAAAATTTCAAAAAATGGAAACTGAGTAATGTCAATTAA

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(68-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TCX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA159

66.139

99.685

0.659
  comYH Streptococcus mutans UA140

65.823

99.685

0.656

Multiple sequence alignment