Detailed information NTG1120080

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: EL070_RS00705
• Genome accession: NZ_LR134284
• Coordinates: 107259..107693 (+)
• Length: 144 a.a.
• NCBI ID: WP_002986542.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC8232
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 102259..112693

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL070_RS00675 (NCTC8232_00134)	-	103928..104293 (+)	366	WP_111705425.1	DUF1033 family protein	-
EL070_RS00680 (NCTC8232_00135)	comYA	104386..105324 (+)	939	WP_002987773.1	competence type IV pilus ATPase ComGA	Machinery gene
EL070_RS00685 (NCTC8232_00136)	comYB	105260..106294 (+)	1035	WP_011054115.1	competence type IV pilus assembly protein ComGB	Machinery gene
EL070_RS00690 (NCTC8232_00137)	comYC	106296..106622 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
EL070_RS00695 (NCTC8232_00138)	comGD	106597..107025 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
EL070_RS00700 (NCTC8232_00139)	comGE	106982..107266 (+)	285	WP_002987779.1	competence type IV pilus minor pilin ComGE	-
EL070_RS00705 (NCTC8232_00140)	comYF	107259..107693 (+)	435	WP_002986542.1	competence type IV pilus minor pilin ComGF	Machinery gene
EL070_RS00710 (NCTC8232_00141)	comGG	107677..108003 (+)	327	WP_002986539.1	competence type IV pilus minor pilin ComGG	-
EL070_RS00715 (NCTC8232_00142)	comYH	108101..109054 (+)	954	WP_002987790.1	class I SAM-dependent methyltransferase	Machinery gene
EL070_RS00720 (NCTC8232_00143)	-	109113..110309 (+)	1197	WP_002986533.1	acetate kinase	-
EL070_RS00725	-	110496..110804 (+)	309	Protein_92	hypothetical protein	-
EL070_RS00730 (NCTC8232_00144)	proC	110887..111657 (-)	771	WP_111705426.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16573.20 Da
• Isoelectric Point: 10.3584

>NTDB_id=1120080 EL070_RS00705 WP_002986542.1 107259..107693(+) (comYF) [Streptococcus pyogenes strain NCTC8232]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1120080 EL070_RS00705 WP_002986542.1 107259..107693(+) (comYF) [Streptococcus pyogenes strain NCTC8232]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	45.985	95.139	0.437
comGF/cglF	Streptococcus mitis NCTC 12261	44.03	93.056	0.41
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396