Detailed information NTG1114728

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AWM52_RS26990
• Genome accession: NZ_LN854573
• Coordinates: 5988855..5989964 (+)
• Length: 369 a.a.
• NCBI ID: WP_032833533.1
• Uniprot ID: -
• Organism: Pseudomonas sp. URMO17WK12:I11 isolate Shine
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5983855..5994964

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AWM52_RS26975 (PSHI_55600)	-	5984821..5985753 (+)	933	WP_027923786.1	sugar kinase	-
AWM52_RS26980 (PSHI_55610)	-	5985869..5986144 (-)	276	WP_007898282.1	peptidylprolyl isomerase	-
AWM52_RS26985 (PSHI_55620)	-	5986196..5988733 (-)	2538	WP_059182855.1	PAS domain-containing sensor histidine kinase	-
AWM52_RS26990 (PSHI_55630)	pilU	5988855..5989964 (+)	1110	WP_032833533.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AWM52_RS26995 (PSHI_55640)	-	5990089..5993940 (+)	3852	WP_059182856.1	hypothetical protein	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41091.09 Da
• Isoelectric Point: 6.2368

>NTDB_id=1114728 AWM52_RS26990 WP_032833533.1 5988855..5989964(+) (pilU) [Pseudomonas sp. URMO17WK12:I11 isolate Shine]
MEIDALLKLLASGNGSDLYLSTAAPPSARFDGVLEPLCDQPFKPGEVAAMAASIMDAEQRLEFDRELEMNLAISLSGIGR
FRVNLFKQRNDVSIVIRNIKLDIPRFEDLKLPPVLLDTVMLKQGLILFVGATDSGKSTSLAALIDYRNRHSSGHIVTIED
PIEYIHRHKQSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPEERRTQLLHDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIADLIRRNELDELKNIMDKSAEFGMQTFDGAL
YALVVEGAISEEEALKHADSLCNLKLRLKLYGAGVSNVNMRSDGWGLVD

Nucleotide

• Length: 1110 bp

>NTDB_id=1114728 AWM52_RS26990 WP_032833533.1 5988855..5989964(+) (pilU) [Pseudomonas sp. URMO17WK12:I11 isolate Shine]
ATGGAAATCGATGCACTGTTGAAACTTCTGGCCAGCGGTAATGGCTCCGATCTTTATCTCTCCACGGCGGCACCGCCCAG
CGCGCGGTTCGACGGCGTCCTTGAACCGTTGTGCGATCAGCCGTTCAAACCCGGTGAAGTCGCGGCAATGGCCGCGTCCA
TCATGGACGCCGAACAGCGTCTGGAGTTCGACCGTGAGCTGGAAATGAACCTGGCCATTTCGTTGTCGGGAATCGGGCGC
TTTCGGGTCAATCTGTTCAAGCAACGCAACGACGTGTCCATTGTGATCCGTAACATCAAACTCGACATCCCGCGCTTCGA
AGATCTCAAATTACCCCCCGTGCTGCTGGACACCGTGATGCTCAAGCAAGGCCTGATACTGTTCGTCGGCGCCACAGACT
CGGGCAAGTCGACATCGCTGGCGGCGCTGATCGACTACCGCAATCGCCACAGCAGCGGTCATATCGTCACCATTGAAGAC
CCGATCGAATATATCCATCGGCACAAGCAGTCGATCATCAATCAGCGCGAAGTGGGCGTCGATACCCGAAGTTTTCATGC
GGCATTAAAAAACACCCTGCGCCAGGCACCGGACGTGGTGCTGATCGGCGAAATTCGTGACCGCGAAACCATGGAGCACG
CGCTGGCGTTCGCCGACACCGGTCATCTGGTGATTTCCACGTTGCACGCACACAACGCCCATCAGGCGCTGGACCGTATC
ATTAATTTCTTTCCCGAAGAGCGGCGGACGCAGTTGCTGCATGACCTGGGCAACAATCTCAAGGCGTTTGTTTCACAACG
ACTGGTGCGTACCCGCGACGGTCAGCGCAGGGCGGCAGTGGAAGTGATGTTGGGTACACCGACGATTGCTGACTTGATTA
GACGCAATGAACTCGATGAACTTAAAAACATTATGGACAAGTCTGCAGAGTTCGGTATGCAAACGTTCGATGGCGCACTG
TATGCGCTGGTGGTGGAAGGCGCAATCAGCGAGGAAGAGGCGCTAAAACATGCGGACTCGCTTTGTAATCTTAAGTTGCG
CCTTAAACTTTATGGCGCAGGGGTTTCGAACGTTAATATGCGCAGTGATGGCTGGGGGTTGGTGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (58-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.014	100	0.55
pilU	Vibrio cholerae strain A1552	50.954	99.458	0.507
pilU	Acinetobacter baylyi ADP1	52.557	95.393	0.501
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	90.244	0.371
pilT	Vibrio cholerae strain A1552	41.141	90.244	0.371
pilT	Pseudomonas aeruginosa PAK	40.237	91.599	0.369
pilT	Pseudomonas stutzeri DSM 10701	39.941	91.599	0.366
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.823	91.87	0.366
pilT	Legionella pneumophila strain ERS1305867	40.12	90.515	0.363
pilT	Legionella pneumophila strain Lp02	40.12	90.515	0.363