Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   LACPI_RS01195 Genome accession   NZ_LN774769
Coordinates   246972..247913 (+) Length   313 a.a.
NCBI ID   WP_047914712.1    Uniprot ID   A0A0D6DUJ7
Organism   Lactococcus piscium MKFS47     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 241972..252913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LACPI_RS01190 (LACPI_0225) - 242455..246849 (+) 4395 WP_047914711.1 PolC-type DNA polymerase III -
  LACPI_RS01195 (LACPI_0226) comGA/cglA/cilD 246972..247913 (+) 942 WP_047914712.1 competence type IV pilus ATPase ComGA Machinery gene
  LACPI_RS01200 (LACPI_0227) comGB 247876..248880 (+) 1005 WP_167594955.1 competence type IV pilus assembly protein ComGB -
  LACPI_RS01205 (LACPI_0228) comYC 248912..249229 (+) 318 WP_082095458.1 competence type IV pilus major pilin ComGC Machinery gene
  LACPI_RS12700 (LACPI_0229) comGD 249201..249629 (+) 429 WP_269448934.1 competence type IV pilus minor pilin ComGD -
  LACPI_RS01215 (LACPI_0230) comGE 249601..249906 (+) 306 WP_047914715.1 competence type IV pilus minor pilin ComGE -
  LACPI_RS01220 (LACPI_0231) comGF 249881..250324 (+) 444 WP_047914716.1 competence type IV pilus minor pilin ComGF -
  LACPI_RS01225 (LACPI_0232) comGG 250336..250620 (+) 285 WP_050702932.1 competence type IV pilus minor pilin ComGG -
  LACPI_RS01230 (LACPI_0233) - 250837..251256 (+) 420 WP_047914717.1 GNAT family N-acetyltransferase -
  LACPI_RS01235 (LACPI_0234) ispE 251355..252206 (+) 852 WP_047914718.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  LACPI_RS01240 (LACPI_0235) - 252336..252785 (+) 450 WP_047914719.1 zinc-dependent MarR family transcriptional regulator -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 34664.80 Da        Isoelectric Point: 9.6230

>NTDB_id=1113968 LACPI_RS01195 WP_047914712.1 246972..247913(+) (comGA/cglA/cilD) [Lactococcus piscium MKFS47]
MVQKLAKTLLQEAVSFGASDVYILPVETTFAVSFRRSESRKHYITCSAAEGQSLISHFKFTAGMNVGEKRRPQLGACTYE
ISERSMRLRLSTAGDFENRESLVIRLLPDQAHPLLFWDEATLLSLKTAVVGRGLYLFSGPVGSGKTTLMHHVAKENFADK
QVITIEDPVELVRPELLQFQLNEAIGNTYDSLVKLSLRHMPDLVIVGEIRDSTTARAVIRASLTGYTVFSTIHAKSVRGV
YARLLALGVTRDELENALSGIIYQRLIAGKGVLTYADKNFSNHSHNGWNRQIEALVRSGSIRASQATVEKIIS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1113968 LACPI_RS01195 WP_047914712.1 246972..247913(+) (comGA/cglA/cilD) [Lactococcus piscium MKFS47]
ATGGTTCAGAAATTAGCAAAAACACTCTTACAAGAGGCTGTTAGCTTTGGTGCAAGCGATGTCTATATTTTACCAGTTGA
GACGACCTTTGCAGTTAGCTTTAGACGTTCGGAGAGTCGTAAGCACTATATCACTTGTTCAGCTGCTGAGGGACAAAGTT
TGATCTCACATTTCAAATTTACAGCTGGTATGAACGTTGGTGAAAAGAGACGTCCGCAGTTAGGTGCCTGTACCTATGAG
ATTAGTGAGCGTAGTATGCGGCTAAGACTTTCTACAGCAGGGGATTTTGAAAACCGAGAAAGTCTGGTCATTCGATTATT
GCCGGATCAAGCGCATCCCCTCTTGTTTTGGGATGAAGCAACTCTTCTGTCACTAAAAACGGCGGTAGTCGGTCGGGGAC
TTTATTTATTTTCAGGACCAGTGGGTTCCGGGAAGACGACGCTAATGCACCATGTTGCAAAAGAGAACTTTGCAGATAAA
CAGGTCATTACGATAGAAGACCCCGTAGAACTTGTCAGGCCAGAGCTTTTGCAATTCCAACTTAATGAAGCGATTGGCAA
CACCTATGATAGCTTAGTTAAACTATCTCTGAGACACATGCCAGATCTTGTCATTGTAGGAGAAATTCGAGACAGTACGA
CAGCTCGTGCGGTCATACGGGCTAGTTTGACGGGATACACGGTCTTTTCGACGATTCATGCCAAATCTGTTCGTGGTGTA
TATGCTAGGTTACTCGCCTTAGGTGTGACCCGGGATGAGCTAGAAAATGCCTTGTCTGGTATTATTTATCAGCGACTCAT
AGCAGGAAAAGGGGTGCTAACCTATGCAGATAAAAACTTCTCTAATCACAGCCATAACGGATGGAATCGGCAGATTGAGG
CACTTGTTAGGTCAGGATCTATTCGTGCTAGTCAGGCGACGGTCGAAAAAATTATCAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6DUJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

54.808

99.681

0.546
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.808

99.681

0.546
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comYA Streptococcus mutans UA140

54.808

99.681

0.546
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.361

0.546
  comYA Streptococcus gordonii str. Challis substr. CH1

54.019

99.361

0.537
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.733

99.361

0.524
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.733

99.361

0.524
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.733

99.361

0.524
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.733

99.361

0.524

Multiple sequence alignment