Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACOB85_RS10465 Genome accession   NZ_CP184834
Coordinates   564437..565660 (-) Length   407 a.a.
NCBI ID   WP_017635918.1    Uniprot ID   A0AAW9BMM9
Organism   Vibrio alginolyticus strain HoVa25     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 559437..570660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOB85_RS10435 (ACOB85_10435) trmD 559931..560692 (+) 762 WP_005385420.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ACOB85_RS10440 (ACOB85_10440) rplS 560734..561087 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  ACOB85_RS10445 (ACOB85_10445) yacG 561767..561961 (-) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  ACOB85_RS10450 (ACOB85_10450) zapD 562120..562860 (-) 741 WP_005373086.1 cell division protein ZapD -
  ACOB85_RS10455 (ACOB85_10455) coaE 562888..563502 (-) 615 WP_420788405.1 dephospho-CoA kinase -
  ACOB85_RS10460 (ACOB85_10460) pilD 563503..564372 (-) 870 WP_420788406.1 prepilin peptidase Machinery gene
  ACOB85_RS10465 (ACOB85_10465) pilC 564437..565660 (-) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  ACOB85_RS10470 (ACOB85_10470) pilB 565684..567369 (-) 1686 WP_420788407.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOB85_RS10475 (ACOB85_10475) pilA 567382..567813 (-) 432 WP_420788408.1 pilin Machinery gene
  ACOB85_RS10480 (ACOB85_10480) nadC 568069..568956 (-) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACOB85_RS10485 (ACOB85_10485) ampD 569049..569600 (+) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45313.50 Da        Isoelectric Point: 10.3992

>NTDB_id=1111380 ACOB85_RS10465 WP_017635918.1 564437..565660(-) (pilC) [Vibrio alginolyticus strain HoVa25]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFISA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=1111380 ACOB85_RS10465 WP_017635918.1 564437..565660(-) (pilC) [Vibrio alginolyticus strain HoVa25]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCAGGCAAAAAGACATCTGGACAAAC
CCTCGCGATGACGGAAATTGAAGTACGCGAGCGTCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TCTCGTTTCTCACTAAACTCAGCCATCGCGTGAAAGGCAGAGACATCACGGTGTTTACCCGTCAGATTTCGACGATGTTG
ATAACCGGTGTACCCTTAGTTCAGGCTTTAAAATTGGTCTCAGAAAATCATAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTTGAAGCGGGGACGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACCGATCTTATCGCCACAGGTGAGCAATCCGGTAACTTAGCAGAAGTGTTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCACTGATTTACCCAGCGATGGTAGTGCTAGTAGCGTTAGGCGTGTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGTTTTGGTGCTGACTTACCGTGGTTTACCAGGC
AAGTGTTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCATTTCGGCG
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAATTTCCTGTTCTGGGCCCTGT
ATTATCAAAAGCCGCTATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGCATTCCAATTCTAACCAGTT
TAAAAACTACATCAAAAACATCAGGAAACTTGCACTATCAACTCGCCATAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTTGCCATGCGTAACTGTAATGTGTTCCCTGAATTGGTGTTACAAATGGTTATGATCGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACGATCTACGAGTTTGAAGTCGACAACACCGTCGACAACCTTAGTA
AAATCCTAGAGCCACTAATTATCGTTTTTTTAGGTATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

87.224

100

0.872
  pilC Vibrio cholerae strain A1552

73.775

100

0.74
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilC Legionella pneumophila strain ERS1305867

40.295

100

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398

Multiple sequence alignment